FastQCFastQC Report
Fri 30 Jun 2023
SRR10446733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10446733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52429539
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length18-95
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCTTGTGTCGAGGGCTGACTTTC5785221.103427592601949No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTC5715951.0902155748498952No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGC4121950.7861884881345227No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCC4022250.767172490301698No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT2946470.5619866312385466No Hit
AACGTCCGTCGTGAAGAGGGAAAC2408860.45944710671592975No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCT2245120.4282166204055313No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACG2145010.4091224223810169No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTC2134850.4071845834845125No Hit
TGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT2042820.38963150143280867No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCT1843940.3516986864980827No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1639310.31266916155795305No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCCCCG1586450.30258705879523373No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1525920.2910420402513934No Hit
AAAAAGCGACGTCGCTATGAACGCTTGGCCGCC1482350.2827318393930567No Hit
GAAGCCGAGCGCACGGGGTCGGCGGCGAT1436570.2740001204282952No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGC1360240.25944153352178057No Hit
GAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAAC1330770.25382065632886835No Hit
TCCGGCCGTGCCGGTGCGGCGGATCTTTCCCGCTC1322650.25227191106906355No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCC1298830.24772867066406973No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAA1285050.24510038129459807No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1279510.24404372504591354No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG1279350.24401320789793707No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGC1230840.23476079009582748No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTC1105670.21088684376950176No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1066330.20338344001079237No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTC1062610.20267391632033996No Hit
GTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT1058300.20185186064672436No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCGGA983080.1875049864542963No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGC950980.18138248364152124No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGA926420.17669810142713632No Hit
AAAAAGCGACGTCGCTATGAACGCTTGGCCGCCAC906500.17289871650406843No Hit
AAAAAGCGACGTCGCTATGAACGCTTGGCCGC890990.16994046047210143No Hit
GGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT889800.16971348918402657No Hit
TGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTC888690.16950177646993997No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCG885300.16885519439718896No Hit
CCGGGCCGCCCCTCCCACGGCGCGACCGCTCT884860.1687712722402537No Hit
CGGACGCCGCCGGAACCGCGACGCTTTCC878140.16748955202524285No Hit
AATGATAGGAAGAGCCGACATCGAAGGATC875390.16696503854439765No Hit
AAATGTCTGAACCTGCGGTTCCTCTCGT861600.16433484185317745No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCT826710.15768019627256305No Hit
GTGCCGGTGGCGGCGGATCTTTCCCGCTC824370.1572338829834075No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCC821710.15672653539829906No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTAC801370.15284704296179297No Hit
ACCTACGGAAACCTTGTTACGACTTTT789060.15049912988935493No Hit
AGGCCCGACCCTGCTTAGCTTCCGAGATC724730.13822932908107394No Hit
AAGGGCCAGCGAGAGCTCACCGGACGC724600.13820453389834306No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAG717160.1367854865174382No Hit
ATGACCCGCACTTACTGGGAATTCCTCGTTC715720.13651083218565016No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAAT705840.13462639829810444No Hit
AGGTGAAGCGACTTGCTCGTGGAGCTGAAAT701070.13371660582405653No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAAC695110.13257984206193382No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCG691570.1319046501629549No Hit
AAAGCGGGTGAAAAGCCCGCTCGCCGGAAGAC687700.13116651664627454No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT683650.13039405133812065No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT682910.13025290952872962No Hit
GGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTC676860.12909897987087013No Hit
AAGAGACAGCTGAACCCTCGTGGAGCC667360.12728702420976848No Hit
CGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTC660800.12603582114273404No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC649630.12390534274962822No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCT641090.12227648997638525No Hit
ACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTC639200.12191600616591346No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCT635160.12114544817950813No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTC613960.11710192607262863No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGC613090.11693598908050669No Hit
GGCGGGAGAGGCCGGGAGGGAGGAAGAC612470.11681773513209795No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCG605120.11541585364692984No Hit
AAGGCGCTTGAGAGAACTCGGGTGAAGGAAC596170.11370880068199722No Hit
GAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACC587220.11200174771706461No Hit
ACGGTCTGTATTCGTACTGAAAATC569530.10862769554391848No Hit
AAGCGTACACGGTGGATGCCCTGGCAGTC558810.1065830466294964No Hit
ACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGAC556170.10607951368788499No Hit
AAGCTTGGGCTGCAGGTCGACCCGTG551570.10520214568356208No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC543540.10367056631949406No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCC533100.10167932241403077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)