FastQCFastQC Report
Fri 30 Jun 2023
SRR10446744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10446744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50170379
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length18-95
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGC44252648.820471537597912No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT18885003.7641732784199218No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC14104712.8113620588754173No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATC8786141.751260439950035No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT8634551.7210454001154747No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCG6921491.379596913150686No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGC6648441.3251723691383714No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC6604811.3164760027027105No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGAT6588221.3131692706566955No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCT6467481.2891032774538138No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC6082301.2123288923131315No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC5206611.0377856623327482No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG5131661.0228465684901444No Hit
CGGGTCGGGGGTCGGCGCGCGGCGCGGG5106921.0179153719368954No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT4487370.8944261712673129No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATC4363560.8697482632132398No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG3973860.7920729480636373No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT3617750.7210928185334219No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCC3549920.7075728887756658No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG3404480.6785836718514724No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT3364790.6706726293616398No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTC3271070.651992284132436No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTC3244050.6466066361587581No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3240820.6459628299798174No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGT3232050.644214786577554No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCC3009730.5999017866697798No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTC2869010.5718533639141933No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTC2818910.5618673919126662No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACG2659270.5300478196507147No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCC2559890.5102393187023762No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGC2477080.4937335633840837No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAT2359040.4702057363369728No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2345580.46752287839005563No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCAC2332070.4648300544032167No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT2321500.46272323356377276No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2121680.42289495162075613No Hit
AGCGCCGAAGGAGCCTCGGTTGGCCCCGGAT2016100.40185066172212885No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCG1919150.38252651031398427No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCC1901650.37903839634139497No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTC1877220.37416898923566033No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAT1811300.3610297622029126No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1750390.34888913237031755No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1622820.32346177811413385No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1540840.3071214590585413No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC1467470.29249729207746267No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCG1465420.29208868444067365No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCT1445330.28808432960014113No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCT1415970.28223227095812853No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGA1391990.277452558211689No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG1379280.274919190863597No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGC1335160.2661251572367033No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCC1319380.26297987503742No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC1305460.2602053295232233No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCG1283150.25575848251016803No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC1249070.2489656296995484No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCCCCG1234620.24608544416218184No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATC1226740.2445147962705245No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCT1219230.24301789707428761No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCG1209060.24099080455421712No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1203690.2399204518666283No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCT1155770.2303689992056867No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1094590.21817455275751457No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCC1080630.2153920344113805No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCCCGTCCG1020750.2034567050011721No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAG974880.19431385997701953No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGC957530.19085564412419528No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCC952930.1899387684514004No Hit
TGGCGCGCGGGCGGGGCCGGGGGTGGGGT922560.18388539580296973No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTC902630.1799129322901866No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC882250.17585077441810834No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT882050.1758109102584216No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG867520.17291477905718033No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAC866680.17274734958649604No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGG809340.1613182950043092No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC806170.16068644807327448No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCC776200.15471280374421728No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTC768370.15315212189248162No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTC761160.15171501893577483No Hit
GGCGGGCGTGGGGGTGGGGGCCGGGCCG742850.14806545511645428No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT741120.14772063013516404No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCC731830.14586893991771518No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCCCCGTCCGCCT727000.14490622046128054No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAA704340.14038961116877352No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCT700920.13970793403813034No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC697420.1390103112436125No Hit
CGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCAC686840.13690149719618422No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT682670.1360703294667158No Hit
TGGTGACTCTAGATAACCTCGGGCCGATC681270.13579128034890867No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGC670820.13370837800527677No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGC662600.132069961042152No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC646850.1289306584668216No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG641170.12779851633171838No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCC637500.12706700900146678No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT622220.12402138720140024No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG622010.12397952983372919No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC620450.12366858938817264No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTC616440.12286931298645361No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCT611210.1218268652106455No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC604240.1204375992455628No Hit
CGCCGAATCCCGGGGCCGAGGAAGCCAGAT585930.11678803542624223No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC576790.11496624332855848No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAT569580.11352914037185168No Hit
GGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT554740.11057121972309597No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG550480.10972211312176852No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCC549250.10947694853969511No Hit
TCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC549180.10946299608380475No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCC543570.10834480640459183No Hit
TCCGGCCGTGCCGGTGCGGCGGATCTTTCCCGCTC541570.1079461648077245No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGA537750.10718475935770787No Hit
TCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGG535800.1067960838007622No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCGT532810.10620011461344551No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC532010.10604065797469857No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCC523160.10427666890856058No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCC520710.10378833295239806No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCC520630.10377238728852337No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCC513690.10238910094739369No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGC513620.10237514849150334No Hit
CCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCAC511360.10192468348704323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)