FastQCFastQC Report
Fri 30 Jun 2023
SRR10446748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10446748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25595343
Total Bases874.3 Mbp
Sequences flagged as poor quality0
Sequence length18-95
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC22345928.730463194027132No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGC14224475.557444571069041No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC7529762.941847663459716No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC5625682.19793108457269No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCCCCG4345781.6978791805993771No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG3416431.3347857850547267No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC3270721.2778574602418884No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATC2793871.0915540377794508No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC2657791.0383881161506605No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT2573371.005405553658726No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2571081.0045108596513046No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT2530240.9885548320254978No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2421740.9461643080930776No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2247050.8779136110815159No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGAT2230580.8714788467573965No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC2212090.8642548763655951No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG2207700.8625397206046428No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2146870.8387736784773699No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGC1922880.7512616650614918No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1912900.7473625182518554No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1604050.6266960360718745No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1543180.6029143661016771No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCC1402100.5477949641073377No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC1321850.5164416042402714No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACG1283370.5014076193470038No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTC1227170.4794505000382296No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGA1073110.41925986301492424No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC1045940.4086446507085293No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATC1037260.40525340879393573No Hit
AGATAACCTCGGGCCGATCGCACGCCCC832650.3253130852749268No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT776090.303215315379833No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAT723900.2828248873242292No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT700790.27379590107466034No Hit
GTCGCGCCGTCGGGTCCCGGGGG669020.26138348683196No Hit
CTTTGGTGACTCTAGATAACCTCGGGCC634900.24805293681745152No Hit
CGGTGGGGGGCGGGGCGGACTGTC617360.24120012769510454No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC609560.23815269832484762No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC580810.22692018622293905No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCA577040.22544726202731488No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAC576020.22504875203274285No Hit
CCGGTGGGGGGCGGGGCGGACTGTC564950.22072374650341664No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG560770.21909063691781744No Hit
TCCGGCCGTGCCGGTGCGGCGGATCTTTCCCGCTC540890.21132359898439335No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC520590.20339246870026317No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCC510890.1996027167910975No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT507000.19808290906670017No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCT501050.19575826743169644No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC498240.19466041146625773No Hit
TCCGGCCGTGCCGGTGCGGCGGATCTTTCCCGCTCCCCG479990.18753020813200277No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT460850.18005228529268No Hit
ACCGCCCGTCCCCGCCCCTTG458570.1791614982459895No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC456400.17831368776734113No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAA455810.1780831770842063No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAC452330.176723554749784No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTC443710.17335575459957697No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCT437190.17080841620290066No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC409190.15986892615582451No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT399380.15603619767861676No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC376030.14691344437150147No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC373530.1459367041887268No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCG372260.1454405201758773No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGT371120.14499512665253206No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG366270.14310025069794924No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTC362300.14154918728770308No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCC361620.14128351395798838No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC361240.14113504945020663No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCC342760.13391498601913637No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGC342390.13377042847208573No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCC338180.13212559800429322No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTC326810.12768338365303405No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGG310850.12144787432620066No Hit
AAACCCTTGTGTCGAGGGCTGACTTTC303370.1185254676993389No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGC302560.1182090038801199No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCG302090.11802537672575827No Hit
GTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG301330.11772844771019478No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCG299480.11700565997494153No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCC295060.11527878333179595No Hit
ACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCTG288670.11278223542462393No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCG285130.11139917132581502No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTC283700.11084047594126792No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGC277260.10832439323044041No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA276740.10812123127242326No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT276690.10810169646876779No Hit
TGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCT276560.10805090597926349No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCC274750.10734374608693464No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCC270930.10585128708765497No Hit
TGGGGGGCGGGGCGGACTGTC266680.10419082877693805No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCC264780.10344850623802931No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC257990.10079567990161337No Hit
GGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT257200.10048703000385656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)