FastQCFastQC Report
Fri 30 Jun 2023
SRR10446756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10446756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11115179
Total Bases279.8 Mbp
Sequences flagged as poor quality0
Sequence length18-40
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAGGCGCCGCCGCCC3422123.078780827551225No Hit
TGGGCGCGCGCCGCGGCTG2175421.957161463616555No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCT2078671.8701183309778455No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1553911.3980071755929437No Hit
ACGAGGCGCCGCCGCCCT1138381.0241670422041786No Hit
CTGGACGAGGCGCCGCCGCCC948550.85338256810799No Hit
CGCTCGGCGGGGTCCCCGCGTCGTCC890500.8011566885247642No Hit
CCAAGCGTCCGTCCCGCG861100.7747063722500556No Hit
ATAACCTCGGGCCGATCGCACGCCCC847180.7621829571975404No Hit
CGCCGTCGGGTCCCGGGGG821040.7386655671492109No Hit
TGGGCGCGCGCCGCGGCTGG810760.7294169531592789No Hit
TCCCCGCTCGGCGGGGTCCCCGCGTCGTCC717420.6454416973401867No Hit
GGCGGGGGGCCCGGACAC697360.6273943046711169No Hit
CTTCTGGCGCCAAGCGTCCGTCCC592160.5327489552799825No Hit
GACGAGGCGCCGCCGCCCT592100.532694975042687No Hit
CGCCAAGCGTCCGTCCCGCG589800.530625732613033No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCC550190.4949897792919034No Hit
CATTTATCAGATCAAAACCAACC538860.4847965111493031No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTC517500.46557954667216783No Hit
CTCGGCGGGGTCCCCGCGTCGTCC506460.4556471830098283No Hit
GGGCGCGCGCCGCGGCTG503510.4529931546761415No Hit
ACCGCCCGTCCCCGCCCCTTG371810.3345065338129058No Hit
ATTCAACCCGGCGGCGCGCG360240.32409734472112417No Hit
GGCGGGGGGCCCGGACCC354690.3191041727713067No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCG354020.31850139345484224No Hit
ATCAGATCAAAACCAACC343940.30943271358922786No Hit
CGCCAAGCGTCCGTCCCG338690.304709442825887No Hit
ACGAGGCGCCGCCGCCCTCTCCCAC335900.30219936179165446No Hit
CCGTCGGGTCCCGGGGGG334990.30138066152600873No Hit
TGCATTTATCAGATCAAAACCAACC312870.28147994737646603No Hit
TGGACGAGGCGCCGCCGCCC310370.2792307708224942No Hit
CGCCCCGGGCGTCGTCGCG304720.2741476318105179No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC304370.2738327470929618No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT297090.26728314496779587No Hit
ACGAGGCGCCGCCGCCCC284820.25624418644090213No Hit
CCGCTCGGCGGGGTCCCCGCGTCGTCC282700.254336884723134No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCC278930.2509451264797445No Hit
CTGGGCGCGCGCCGCGGCTG273190.24578101711182518No Hit
AGGGGCTCTCGCTTCTGG255680.23002778452780653No Hit
CTTCTGGCGCCAAGCGTCCGTCCCG251800.22653706251604228No Hit
ATTTATCAGATCAAAACCAACC250780.22561939848202175No Hit
TGGGCGCGCGCCGCGGCT245380.2207611771254426No Hit
ATTCAACCCGGCGGCGCGC243630.21918675353766234No Hit
GTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC243380.21896183588226512No Hit
GCGCCAAGCGTCCGTCCCGCG241990.21771129371825682No Hit
TGGCGCGCGGGCGGGGCCGGGGGT241570.21733343205718952No Hit
ACCGCCCGTCCCCGCCCCTTGCCT232970.20959626471152648No Hit
CGCCAAGCGTCCGTCCCGC225300.2026957910439409No Hit
ACGAGGCGCCGCCGCCCTCTCCC215630.1939959761331779No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT214730.193186272573748No Hit
GCGCCAAGCGTCCGTCCC214210.1927184438505219No Hit
ACCGCCCGTCCCCGCCCCTTGC213930.19246653607647704No Hit
CCTCCCGACCCCTACCCGCGCGTCG213740.19229559865837517No Hit
AGTTCTACAGTCCGACGATC213040.19166582922326308No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTC211390.19018137269764165No Hit
ATGCGTGCATTTATCAGATCAAAACCAACC202280.18198537333496834No Hit
GGGCGCGCGCCGCGGCTGG192580.17325856830555766No Hit
CGTGCATTTATCAGATCAAAACCAACC183040.16467571057560115No Hit
CAGAGTTCTACAGTCCGACGATC178920.1609690676146556Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCTACAGTCCGACGATC178050.1601863541738734Illumina DpnII expression Sequencing Primer (100% over 18bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTC177620.15979949580659025No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCT174610.15709148723560817No Hit
GGCCCAAGTCCTTCTGATCGAGGCC168500.15159449973770103No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCG168290.1514055689071674No Hit
CTACTTGGATAACTGTGGTAATTCTAG167800.15096473030258892No Hit
CGCTCGGCGGGGTCCCCGCGTCGTC167450.15064984558503286No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCC161120.1449549305503762No Hit
TCCCCCGCCTCCCCTCCGC159350.14336251355016416No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCT157440.14164414266292968No Hit
CGGGGTCCCCGCGTCGTCC156000.1403486169678419No Hit
GTTCTACAGTCCGACGATC150670.13555337255477395Illumina DpnII expression Sequencing Primer (100% over 19bp)
CTTCTGGCGCCAAGCGTCCGTCCCGC148530.13362807742457408No Hit
TCGCGCCGTCGGGTCCCGGGGG147960.1331152651702685No Hit
GACGAGGCGCCGCCGCCCC147280.13250348914758817No Hit
CGCCGTCGGGTCCCGGGGGG146350.13166679546951066No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCC146350.13166679546951066No Hit
CTGGACGAGGCGCCGCCGCCCT144960.13041625330550233No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC144310.12983146740146967No Hit
GGGCCCAAGTCCTTCTGATCGAGGCC144210.12974150033931078No Hit
AGAGTTCTACAGTCCGACGATC140510.12641271903943246Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACCTCGGGCCGATCGCACGCCCC139470.1254770615929802No Hit
CTCGGCGGGGTCCCCGCGTCGTC124400.11191902532563802No Hit
ATAACCTCGGGCCGATCG122240.10997573678300637No Hit
CTGGGCGCGCGCCGCGGCTGG121710.10949891135356435No Hit
TCCTCCCGGGGCTACGCCTG121480.10929198711059894No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGT121290.10912104969249709No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTC119130.10717776114986544No Hit
ACCGCCCGTCCCCGCCCCTT119110.10715976773743364No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC117610.10581026180505056No Hit
ACGAGGCGCCGCCGCCCTCTC117380.10560333756208515No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC117180.10542340343776739No Hit
CATTTATCAGATCAAACCCAACC115800.10418185797997495No Hit
GCGCCAAGCGTCCGTCCCG114640.10313824005893202No Hit
TCCGGCCGTGCCGGTGGTCGGCGGATCTTTCCCGCTC113950.10251746733003579No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCC113000.1016627802395265No Hit
CATTTATCAGATCAAAACCAAC111350.1001783237139051No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)