FastQCFastQC Report
Fri 30 Jun 2023
SRR10446760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10446760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11841533
Total Bases358.7 Mbp
Sequences flagged as poor quality0
Sequence length18-40
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGAGTTCTACAGTCCGACGATC3526632.978187030344804Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT2265291.9130040004110955No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC1863981.5741036232386465No Hit
AGTATGGGCATTTCGCAGCCTACCGTGGTGTGGAC1621681.3694848462610372No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAC1234681.0426690530693956No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1217061.0277892228987582No Hit
GGTGGACATCACTTACGCACTTACGC1177330.994237823768257No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1105140.9332744333018369No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1081250.9130996805903424No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1080930.9128294453091503No Hit
GGTGGACATCACTTACGC1068120.9020115892089309No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1061350.8962944240412116No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1024700.865344039492184No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC908630.7673246360923033No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC722320.6099885884707664No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC692690.5849664904028895No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT635640.5367886066778684No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG617090.5211234052212665No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGT576610.48693864215047156No Hit
CGCTCGGCGGGGTCCCCGCGTCGTCC503100.42486053114913414No Hit
AGAGTTCTACAGTCCGACGATC447810.3781689414706694Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC398990.33694117138380647No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTC375000.3166819701469396No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC346680.2927661477614427No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCC343500.2900806846545967No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC328600.2774978543740916No Hit
CCCGGATAGCTCAGTCGGTAGAGCATCAGAC326620.2758257735717157No Hit
AGTATGGGCATTTCGCAGCCTACCGTGGTG323470.2731656450224815No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTC314860.2658946269879077No Hit
GTTCTACAGTCCGACGATC313010.2643323292685162Illumina DpnII expression Sequencing Primer (100% over 19bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC308830.26080238090794494No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT308200.2602703551980981No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGC295030.24914848440653758No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGT282640.23868531211288269No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC281610.23781549230154575No Hit
TCCCCGCTCGGCGGGGTCCCCGCGTCGTCC262070.2213142504437559No Hit
GGTGGACATCACTTACGCACTTACGCACTTACGC250540.21157733546830465No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC248740.21005726201159935No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT246710.20834295694653723No Hit
GTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC231990.19591213401170274No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC225460.19039764530487732No Hit
GTTCAGAGTTCTACAGTCCGACGAC224390.18949404608339138Illumina DpnII expression Sequencing Primer (95% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT218610.1846129213168599No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCC210500.17776414590914877No Hit
GTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC209900.17725745475691365No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAT201430.17010466465786145No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGAT182700.15428745585558898No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC178290.15056327588666096No Hit
GTTCAGAGTTCTACAGTCCGCGATC178250.15052949647651195No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATT175760.14842672819473626No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT175190.14794537160011292No Hit
CAGAGTTCTACAGTCCGACGATC172940.1460452797792313Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATT171660.14496433865446307No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC168460.14226198584254252No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGT166440.14055612563001765No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGC165380.139660971261069No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC155600.1314019054796368No Hit
GTTCAGAGTTCTACAGTCCGAGATC148760.1256256263441566Illumina NlaIII expression Adapter 1 (95% over 24bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC140430.11859106418062593No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGC135570.1144868658475216No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCT134170.11330458649230636No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCT133950.11311879973648684No Hit
CCGCTCGGCGGGGTCCCCGCGTCGTCC132000.11147205349172273No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGC130090.10985908665710765No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTC129190.10909904992875501No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTC128250.10830523379025334No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCC126540.1068611640063833No Hit
CGCCGCGGCTGGACGAGGCGCCGCCGCCC123960.10468239205177236No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCC123130.10398146929118046No Hit
ATTTCGCAGCCTACCGTGGTGTGGAC122970.10384635165058442No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCC121240.10238539216163987No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC120760.1019800392398518No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG119660.1010511054607541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)