FastQCFastQC Report
Fri 30 Jun 2023
SRR10446778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10446778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22214462
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAAACCATCCTCTGCTACCATGGAATTCTCGGGTGCCAAGGAACTCCA4446422.001587974536588RNA PCR Primer, Index 1 (100% over 28bp)
TCGATTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAAGGAACTCCA962130.43310974625448956RNA PCR Primer, Index 1 (100% over 28bp)
TCGAAACCATCCTCTGCTACCTGGAATTCTCGGGTGCCAAGGAACTCCAG924090.4159857663894808RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCGTTAGTATAGTGGTGAGTATCCTGGAATTCTCGGGTGCCAAGGAA772970.3479580104168177RNA PCR Primer, Index 1 (100% over 23bp)
TCGATTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCA651600.29332243112617357RNA PCR Primer, Index 1 (100% over 28bp)
TAATTAAAACAAAGCATCGCGAAGGCCCTGGAATTCTCGGGTGCCAAGGA555750.25017486356410523RNA PCR Primer, Index 1 (100% over 22bp)
TCGAAACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCA555730.2501658604201173RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTTGGAATTCTCGGGTGCCAAGGA496120.22333198976414553RNA PCR Primer, Index 1 (100% over 22bp)
TCGAATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCA456730.20560029767995283RNA PCR Primer, Index 1 (100% over 28bp)
GCGTTGGTGGTATAGTGGTGAGCATATGGAATTCTCGGGTGCCAAGGAAC288740.12997838975348583RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA260730.11736948659841503RNA PCR Primer, Index 1 (100% over 22bp)
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGGAATTCTCGGGTGCCA252060.11346662367965517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)