FastQCFastQC Report
Tue 18 Jul 2023
SRR10486170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10486170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98814359
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length20-60
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG31876793.2259269120998906No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG30439353.0804581751119797No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATGC26265752.658090409714645Illumina Paired End PCR Primer 2 (100% over 29bp)
CGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC11305111.1440756297371721No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG6446770.6524122673305001No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGT5593040.5660149047771489No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA4875410.4933908441383503No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTG4642380.4698082391041974No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACTGGAATTCTCGGG4005770.40538339169917603No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGTG3919800.3966832391231724No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC3791300.3836790561987049No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT3723670.37683490918561746No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGC3313120.3352873037409472No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCAAGG3300140.3339737294657753Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGGAATTCTCGGGT3210390.32489104139207137No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG3139880.3177554387616885No Hit
GGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG3114860.31522341808643417No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGT3099820.3137013720850024No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGTG3010970.30470976389170323No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA2981340.301711211829042No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGT2916350.29513423246514203No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2810850.2844576464843535No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2446500.24758547490046462No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGGT2338170.2366224932957365No Hit
AGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG2236100.22629302285915756No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCTCCAGACTGGAATTCTCGGGTGCC2235270.22620902696945086No Hit
AGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATGC2199020.2225405317864785Illumina Paired End PCR Primer 2 (100% over 29bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCC2160390.21863118091976896No Hit
CGCGGGGCCTCGCTCCGCCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC2099780.21249745697383918No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGGT2068760.20935823709588605No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAACTGGAATTCTCGGGTGCCAA2023410.20476882312215372No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTG1979060.2002806090155379No Hit
TCGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGC1943020.19663336580466No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCA1912920.19358724980445402No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGG1882240.19048243788132047No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCA1823240.18451164572144824No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA1815630.18374151473269182No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCCA1802140.18237632852529054No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG1754700.1775754068292848No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG1713030.17335840836654115No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACTGGAATTCTCGGG1681180.1701351925988813No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGC1616120.16355112924428322No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA1592990.16121037631787907No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGG1567620.158642935689134Illumina Small RNA Adapter 2 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCAA1554600.15732531341927744No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1524480.1542771734217291No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCC1451100.14685112717272192No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1393620.14103415881086676No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA1331430.13474053907489297No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCC1327820.13437520755460247No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC1321510.13373663639309746No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTTGGAATTCTCGGGTG1301680.13172984302817772No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTTGGAATTCTCGG1292690.13082005622280057No Hit
TCGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGGAATTCTCGGG1277230.1292555062771798No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGGAATTCTCGGGTGC1273810.12890940273164148No Hit
CCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCTGGAATTCTCGGGTG1241750.12566493499188716No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1208430.12229295541956609No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATTGGAATTCTCGGGTGC1186030.12002607839615698No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1184680.11988945857554974No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACTGGAATTCTCGGGT1164990.11789683319202626No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA1141250.11549434834668107No Hit
TGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGTG1134090.11476975729812709No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTTGGAATTCTCG1129390.11429411792267963No Hit
CCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGT1126350.11398647032664555No Hit
TCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCG1118070.1131485354269211No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1117970.11313841544020944No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC1072420.10852876149305386No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1062040.10747830687238481No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCCCAGACTGGAATTCTCGGGTGCCA1053820.10664644396468737No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA1039070.1051537459247193No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTTTGGAATTCTCGGGTGCCAAG1028120.10404560737979386No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAATTCTCGGGTGCC1012240.1024385534899842No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC1001160.1013172589623336No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC988870.10007351259547208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)