FastQCFastQC Report
Thu 24 Aug 2023
SRR10486171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10486171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103766993
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length20-60
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG37334253.5978926362451307No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG34101333.2863369183300897No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATGC30606092.9495014855060897Illumina Paired End PCR Primer 2 (100% over 29bp)
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTG7818280.7534457512901044No Hit
CGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC7331200.7065059695812906No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGT6469920.623504624442572No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA5863060.5650216731249021No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT5709930.5502645720879664No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGT5344630.5150606995039356No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG5329890.5136402092715552No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGTG5166300.49787508056632224No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGC5144630.4957867479112554No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG4808440.4633881989815393No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGGT4481700.4319003442645774No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGG4335960.41785541573899126No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCC3684070.35503293422022936No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGGT3288110.31687436485704085No Hit
GGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG3193650.30777127751981787No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCTCCAGACTGGAATTCTCGGGTGCC3021710.2912014613355906No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGC2919950.28139487476523484No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACTGGAATTCTCGGG2820970.27185619612201734No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA2730780.2631646076512981No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTG2699740.26017329036411413No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCCA2671170.2574200063790998No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2539430.24472425446500123No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA2538530.24463752168283415No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCA2492660.24021704088505294No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGTG2425430.23373810205717344No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG2417190.232944015251555No Hit
AGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG2351490.22661252215335953No Hit
CGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGGAATTCTCGGGT2322190.22378888824503185No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2091380.2015457844094991No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCC2077560.20021395435444486No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA2064500.19895536531544283No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA2042190.19680535601527935No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG1958490.18873920727374263No Hit
AGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATGC1951890.18810316687118417Illumina Paired End PCR Primer 2 (100% over 29bp)
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGT1769190.17049641209127067No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC1718450.16560661057220769No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCC1690880.16294969634515669No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA1585920.15283472654931804No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTTGGAATTCTCGGGTG1487880.14338663547858616No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1422630.1370985087714742No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1414780.13634200617146147No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAATTCTCGGGTGCC1411500.1360259133653415No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC1380250.1330143584289852No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTTTGGAATTCTCGGGTGCCAAG1342110.12933881586026108No Hit
TTCCGGCCGTGCCGGTGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGTG1325820.12776895250303724No Hit
CCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGT1319160.12712712991500102No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1314170.12664624482276363No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCCCAGACTGGAATTCTCGGGTGCCA1298510.12513709441305676No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC1293800.12468319285304913No Hit
TTCCGGCCGTGCCGGTGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGGT1293780.12468126545788986No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1280050.12335810868105238No Hit
TCGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGC1265090.12191641710191989No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACTGGAATTCTCGGG1252130.1206674650387142No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1248790.12034559004711642No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGGAATTCTCGGGTGC1236020.11911494823792379No Hit
GCTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTGGAATTCTCGGGTGCC1230100.11854443927078046No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGTGCC1188780.1145624408717327No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCC1174310.11316797047400226No Hit
GGAGGCGTGGGTTCGTATCCCACTTCTGACACTGGAATTCTCGGGTGCCA1165300.11229967895475203No Hit
CCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCTGGAATTCTCGGGTG1164900.11226113105156665No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCA1142880.11013906898121255No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGG1138350.10970251397763833No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCAAGG1137700.10963987363496214Illumina Small RNA Adapter 2 (100% over 21bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGGAATTCTCGGGTGCCAA1127920.10869737740208006No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTGCCA1122100.10813650541073308No Hit
TGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGTG1091680.10520493737348638No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAACTGGAATTCTCGGGTGCCAA1089990.10504207248252823No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1082440.10431448080990456No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACTGGAATTCTCGGGT1074650.10356376039536966No Hit
GGCTTCTGTCGTGAGTGGCACACGCAGGGCAACTGGAATTCTCGGGTGCC1049620.1011516253535457No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTGCC1038720.10010119499174464No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)