FastQCFastQC Report
Thu 24 Aug 2023
SRR10486172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10486172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94434528
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length20-60
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG32032883.392072865551888No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG32016453.390333035815036No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATGC9119280.96567221684001Illumina Paired End PCR Primer 2 (100% over 29bp)
CGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC7887370.8352209903564086No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTG5862880.6208407162261668No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT5857410.6202614789370261No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA5711720.6048338590732407No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGT5340440.5655177309722986No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG5148080.5451480627933037No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGC5079740.5379113029505479No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG4991220.5285376128527906No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACTGGAATTCTCGGG4886570.5174558610596328No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA3216060.34055975797327015No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG2946290.31199287616495525No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA2904260.30754217355753605No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGTG2894910.3065520695989501No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGT2827540.2994180264235556No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTG2645850.28017824158553534No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA2576390.2728228810546922No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC2511950.2659991057508118No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2509980.26579049561194396No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA2478980.2625077979952417No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2436800.25804121136709657No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGGT2436060.25796285019818177No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCC2396530.2537768812695289No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA2389300.2530112714705367No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCTCCAGACTGGAATTCTCGGGTGCC2384130.252463802222848No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGGT2352050.2490667396569187No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGC2332030.2469467523573581No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACTGGAATTCTCGGG2326100.24631880407132442No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGG2305620.2441501057748708No Hit
AGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG2255730.23886708048141037No Hit
GGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG2212420.23428083423046284No Hit
CGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGGAATTCTCGGGT2073700.21959129186307785No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG2033440.21532802070022522No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCCA2024210.21435062395822002No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA1907830.2020267417442908No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1867170.19772111319283558No Hit
CGCGGGGCCTCGCTCCGCCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC1840750.1949234076756332No Hit
TTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTGGAATTCTCGGGTGCCA1789150.1894593045458966No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCA1770990.18753627910333814No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCA1664090.17621626699929077No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCC1654690.17522086836712944No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTGCCA1653970.17514462506764475No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1634940.1731294723048756No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1577560.1670533049098313No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1531950.16222350367441873No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCAA1469860.1556485780285787No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC1465160.15515087871249805No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTTTGGAATTCTCGGGTGCCAAG1433920.15184276666263424No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCC1402590.14852512420033484No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCAA1324280.140232606446659No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGTGCC1320400.13982173977721368No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCACTGGAATTCTCGGGTGC1271420.13463507754282417No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA1218340.1290142520752579No Hit
TCGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGC1213320.12848266684829515No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCAAGG1211920.128334415988186Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTGGAATTCTCGG1196720.12672483522128686No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACTGGAATTCTCGGGT1188890.12589568933939077No Hit
CCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCTGGAATTCTCGGGTG1175010.12442588795488023No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGTG1174910.12441529860772958No Hit
GGAGGCGTGGGTTCGTATCCCACTTCTGACACTGGAATTCTCGGGTGCCA1174430.12436446974140646No Hit
GCTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTGGAATTCTCGGGTGCC1140030.12072173432158204No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1124680.1190962695339569No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAATTCTCGGGTGCC1118130.11840266729558917No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCAA1116890.11827135939092108No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCA1113170.1178774356769168No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA1107080.11723254443544208No Hit
TCTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTGGAATTCTCGGGTGCC1096820.11614607741778515No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA1090420.11546835920014341No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCC1084090.11479805352550712No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTGCC1068430.11313976176171496No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCCCAGACTGGAATTCTCGGGTGCCA1060910.11234344285598591No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG1047490.11092235246836836No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATTGGAATTCTCGGGTGCC1025200.10856198698848793No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA1024960.10853657255532638No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1016720.10766401035011261No Hit
CCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGT1015150.10749775759984737No Hit
TCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCG1008740.10681898044749055No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCACTGGAATTCTCGGGTG1007820.10672155845370455No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA998320.10571557047439259No Hit
CTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTGGAATTCTCGGGTGCCA988100.10463333919559592No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGT965510.10224120567426355No Hit
TTCCGGCCGTGCCGGTGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGTG960370.10169691323072001No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC959050.10155713384833141No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC951740.10078305257161874No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)