FastQCFastQC Report
Tue 25 Jul 2023
SRR10486173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10486173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94886936
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length20-60
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG21496882.2655257832332154No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG20908072.2034719300030936No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATGC12892441.3587160196636554Illumina Paired End PCR Primer 2 (100% over 29bp)
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGT8607600.9071427915008238No Hit
CGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC5105950.5381088498842455No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTG4759650.5016127826068701No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG4693290.49461919605033927No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACTGGAATTCTCGGG4611120.4859594159516333No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC4322780.455571671109709No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA3835870.404256914776972No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGT3049650.3213983008156149No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGTG2813820.2965445106162981No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGC2760480.29092308344744106No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT2591420.2731060891248506No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTG2562320.27003928127682403No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG2522420.26583427670169474No Hit
CGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGGAATTCTCGGGT2478370.2611919094953177No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGG2339150.24651971057427757No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACTGGAATTCTCGGG2319660.24446568703620064No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCTCCAGACTGGAATTCTCGGGTGCC2200380.23189493651686677No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGGT2143510.22590148763998447No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGC2096490.2209461163336542No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCC2023480.21325169568126848No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA1941460.20460772387043882No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1877010.19781542951286782No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGTG1846640.1946147781608208No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1835480.1934386415428147No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA1765460.1860593327620991No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGGT1693930.1785208872167608No Hit
CCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGT1680750.1771318656553522No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA1624780.17123326650572845No Hit
GGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG1623010.17104672870878662No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCC1602880.16892525647577028No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCCA1575030.1659901843600472No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGTGCC1573020.1657783533025031No Hit
AGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG1516330.15980387437107255No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCA1514490.15960995937312172No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1513930.15955094176504972No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGT1491950.1572345006482241No Hit
TCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCG1365290.1438859823653701No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG1342920.1415284397000658No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTTGGAATTCTCGGGTG1338000.14100992785771901No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCA1337520.14095934133651444No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACTGGAATTCTCGGGT1327200.13987173113061635No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1323240.1394543923306787No Hit
TCGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGGAATTCTCGGG1315960.13868716342574283No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTTTGGAATTCTCGGGTGCCAAG1289790.1359291441342357No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCC1232990.12994307245836245No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1229700.1295963440109395No Hit
CGCGGGGCCTCGCTCCGCCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC1204410.12693106667497409No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA1196150.12606055695591226No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1179810.12433850746324025No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG1139030.12004076093256927No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCAAGG1126260.1186949486913562Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAACTGGAATTCTCGGGTGCCAA1115210.11753040481779282No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCAA1094900.11538996263932477No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTTGGAATTCTCGG1065920.11233580142159928No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC1059250.11163285955402755No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTGGAATTCTCGG1055430.1112302751561079No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA1031500.10870832629688876No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTTGGAATTCTCG1026190.10814871290606327No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC972410.10248091475943538No Hit
TCGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGC971560.10239133446146896No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA961160.10129529316870343No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCCCAGACTGGAATTCTCGGGTGCCA959890.10116144966468303No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGG952200.10035101143955158No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)