FastQCFastQC Report
Thu 24 Aug 2023
SRR10486174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10486174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95612156
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length20-60
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG35664003.7300696367520465No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG32277453.3758730427541033No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATGC29732363.109684086613422Illumina Paired End PCR Primer 2 (100% over 29bp)
CGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC9435220.9868222195512462No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTG7965410.8330959506864377No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG6605580.6908724032956646No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGC6143330.6425260403080963No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGT5592360.5849005224816811No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT5552860.5807692486298499No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA5036310.526743691461157No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG4577920.47880104282974223No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCAAGG4433360.4636816264241547Illumina Small RNA Adapter 2 (100% over 21bp)
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACTGGAATTCTCGGG4367340.45677664668496754No Hit
GGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG3704130.3874120357666655No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA3516290.3677659982900082No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGTG3351170.35049622769723965No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGT3048550.31884544053164116No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGTG3040220.3179742124003563No Hit
CGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGGAATTCTCGGGT2995880.31333672676516156No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCC2825560.2955230922729114No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGGT2795490.2923780946849478No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC2743760.2869676947772206No Hit
AGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG2671550.27941530781922747No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCCA2447640.25599673748597407No Hit
AGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATGC2398960.2509053346731351Illumina Paired End PCR Primer 2 (100% over 29bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCA2304560.24103211311331585No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTG2256260.23598045420082356No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG2235350.23379349378963907No Hit
CGCGGGGCCTCGCTCCGCCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC2183070.22832556981562052No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCA2170560.22701715878052162No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGG2158670.2257735930565147Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG2105550.2202178141448876No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2080640.21761249688794804No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGGT2026470.2119468993043102No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2025550.21185067722978654No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGT2003760.2095716783125359No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA1991590.20829882760932616No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCAA1960630.20506074562318205No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA1939840.20288633591737018No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGC1805990.18888706996629173No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGG1764250.18452151628083777No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC1738480.18182625230206084No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAACTGGAATTCTCGGGTGCCAA1716980.17957758425612744No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCTCCAGACTGGAATTCTCGGGTGCC1693450.17711660011097335No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA1682580.175979715382634No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTTTGGAATTCTCGGGTGCCAAG1652840.17286923223444517No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCC1590140.16631148867723472No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCC1572160.16443097465556578No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACTGGAATTCTCGGG1565860.16377206262350155No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA1456570.15234150770535915No Hit
CCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCTGGAATTCTCGGGTG1405210.14696980580586427No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTGCCA1368130.14309163784571494No Hit
TCGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGC1345300.14070386614856797No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTTGGAATTCTCGG1307800.13678177071961437No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1271240.13295798914941317No Hit
TCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCG1267300.13254590765634447No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTGGAATTCTCGG1239010.12958707886474183No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTTGGAATTCTCGGGTG1228240.12846065305754636No Hit
TGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGTG1227890.1284240468335428No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1212330.12679663870355565No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA1208400.1263856031026013No Hit
TTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTGGAATTCTCGGGTGCCA1207420.12628310567539133No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGTGCC1197670.1252633608638634No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGGAATTCTCGGGTGC1183700.12380224958006385No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG1134710.11867842411167887No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC1132690.11846715390457256No Hit
CCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGT1124780.11763985324209193No Hit
TTCCGGCCGTGCCGGTGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGTG1094680.11449171797778518No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTTGGAATTCTCG1077110.1126540855328061No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCA1062870.11116473516191812No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCAA1051560.10998183118054572No Hit
TCGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGGAATTCTCGGG1048330.10964400802759851No Hit
TTTTCCCGCTCCCCGCCTCCCGACCCCTTGGAATTCTCGGGTGCCAAGG1045960.10939613159648863Illumina Small RNA Adapter 2 (100% over 21bp)
TATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGT1041620.1089422144188444No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC1029220.10764530819700373No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCTGGAATTCTCGGGTGCCA1019140.10659104894570101No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACTGGAATTCTCGGGT1008440.10547194438330625No Hit
TATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTG1000160.10460594571259328No Hit
GAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGTGC989890.10353181451111719No Hit
CGCGGGGCCTCGCTCCACCCCCCCCATCGCCTCTTGGAATTCTCGGGTGC986400.1031667981631959No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTGCC966610.10109697766882278No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCTGGAATTCTCGGGTGCC962230.10063887692272099No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)