FastQCFastQC Report
Thu 24 Aug 2023
SRR10486175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10486175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97175035
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length20-60
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG29873783.0742237448126466No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG19659842.023136909598232No Hit
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATGC19572942.0141942835420643Illumina Paired End PCR Primer 2 (100% over 29bp)
CGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC7303840.7516169147765164No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT5229350.538137187190105No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC5058890.5205956447558778No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG4487770.46182334794116614No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGC3723660.38319101197133604No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGT3587640.3691935896910148No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG3221050.331468879841412No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTG3036040.31243003925854No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGTG3025460.3113412822542333No Hit
GGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG3008990.3096464024942209No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGGT2960680.30467496101236285No Hit
CGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGGAATTCTCGGGT2942160.30276912171937986No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA2856770.2939818853679857No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGTG2854940.29379356539465096No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCCA2846100.29288386672564615No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACTGGAATTCTCGGGT2680320.27582392946912754No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCC2641120.271789971570373No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGGT2553820.2628061826785038No Hit
ACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCTGGAATTCTCGG2488490.2560832625375437No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACTGGAATTCTCGGG2361800.24304596339996173No Hit
CGCGGGGCCTCGCTCCGCCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC2321250.23887308093071433No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCA2250040.2315450671049411No Hit
AGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG2152750.22153323639142503No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTTGGAATTCTCGGGTG2083560.21441309488594473No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGC2079080.21395207112608708No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCTGGAATTCTCGGGTG2043570.21029784038667956No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGT2035860.20950442672853167No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGG2022990.20818001248983342No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA1957740.20146532491601365No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACTGGAATTCTCGGGTGCCAA1840830.18943445711133525No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTG1828600.1881759034097595No Hit
TAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCTGGAATTCTCGGGTGCCA1818600.18714683251722009No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC1749940.18008123176904436No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAACTGGAATTCTCGGGTGCCAA1710960.1760699134299257No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA1656670.17048308755432917No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCA1599760.16462664510488728No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGT1589900.1636119812048434No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA1524050.15683554937747127No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCAA1395950.14365315124404124No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1390020.14304291220476534No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCC1362190.14017900791082813No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG1340480.13794489500312504No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCTCCAGACTGGAATTCTCGGGTGCC1334130.13729143498636248No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAATTCTCGGGTGCC1330320.13689935897630498No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCAA1307440.13454484477417478No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC1304310.13422274558480993No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG1290150.1327655812009741No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATTGGAATTCTCGGGTGC1290100.1327604358465114No Hit
TGGACGGCGCCGGACGCGCACGCCCCGCCGGGCTGGAATTCTCGGGTGCC1240020.12760684881667395No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA1199760.12346380940331023No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGGAATTCTCGGGTGCCAA1193100.12277844818887897No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGC1172040.12061122488919093No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCAAGG1159670.11933826419511966Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA1152040.11855308310411208No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTTGGAATTCTCGG1133460.11664106938577383No Hit
GAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCTGGAATTCTCGGGTG1133160.11661019725899763No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG1111200.11435035757898107No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCTGGAATTCTCGGGTGCC1102390.11344374612265384No Hit
TTCCGGCCGTGCCGGTGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGTG1097140.11290348390407064No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1085990.11175606985888917No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACTGGAATTCTCGGG1082840.11143191252773925No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1059050.10898375287438795No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1048410.10788882144472599No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCTGGAATTCTCGGGTGC1028760.10586669714088602No Hit
ACGGGCCCCTCTCTCGGGGCGAACCCATTCCTGGAATTCTCGGGTGCCAA1027100.10569587137272449No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACTGGAATTCTCGGGT1022110.10518236499734732No Hit
GGCTTCTGTCGTGAGTGGCACACGCAGGGCAACTGGAATTCTCGGGTGCC1014030.10435087571617546No Hit
ACCACCACGCCCGCGGTCGGCGGGAGAGGCTGGAATTCTCGGGTGCCAAG1009220.103855892616864No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGC992810.10216718728220679No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG977160.1005566913353826No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTTGGAATTCTCGGGTGCC975360.10037145857472549No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)