FastQCFastQC Report
Tue 18 Jul 2023
SRR10486176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10486176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93654696
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length20-60
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATGC44235404.723244203365947Illumina Paired End PCR Primer 2 (100% over 29bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG40689884.344670554480258No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG32308963.449796046532466No Hit
CGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC8309220.8872187252628527No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT6185550.6604634112527576No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG4766700.5089654020125163No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA4366850.4662713335805393No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC4325360.4618412300436061No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG3871610.41339197769645214No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGT3778720.40347362827380273No Hit
AGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATGC3629220.3875107341120407Illumina Paired End PCR Primer 2 (100% over 29bp)
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGGT3579020.3821506184804657No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA3486340.3722546918522911No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACTGGAATTCTCGGG3399040.36293321586351635No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGC2861020.3054860164192941No Hit
GGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG2814350.30050281728531797No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCC2749820.29361261286887314No Hit
CGGCGGATCTTTCCCGCTCCCCGCCTCCCGACCCCTTGGAATTCTCGGGT2661890.2842238684966742No Hit
AGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGGG2648900.28283685849559537No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGTG2553090.2726067254545357No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTG2486980.2655478162034715No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGGT2414300.25778739381098414No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTTGGAATTCTCGGGT2385080.2546674221226451No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCAA2237880.23895011094798704No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCCA2185340.2333401413208367No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA2091470.2233171521906387No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGG2088950.22304807865694212No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGC1936520.20677233312465185No Hit
CGCGGGGCCTCGCTCCGCCCCCCCATCGCCTCTTGGAATTCTCGGGTGCC1906660.20358402530077083No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGGAATTCTCGG1810630.19333040171311858No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACTGGAATTCTCGGG1810280.19329303038899406No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1790170.19114578088001055No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTG1736250.1853884614605978No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1709050.18248417570006312No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA1678060.17917521188686578No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA1657180.1769457454648083No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1622370.17322890034259467No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCCA1584960.1692344396697417No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCA1581720.16888848798356038No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTTGGAATTCTCGGGTGCCAA1569990.16763601474932982No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAATTCTCGGGTGCC1557720.16632588290073572No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG1512050.1614494589785439No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCA1467230.15666379398636882No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG1288390.1375681151108536No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1281370.13681855312412736No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1265900.1351667405978233No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCC1264220.13498735824202557No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTTGGAATTCTCGG1249160.13337932355255308No Hit
TCGCGGGGCCTCGCTCCACCCCCCCATCGCCTCTTGGAATTCTCGGGTGC1243390.1327632305805573No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTGGAATTCTCGGGTGCC1233650.13172324001777766No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTGCCA1159570.12381333232879213No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCTCCAGACTGGAATTCTCGGGTGCC1131890.12085779446660101No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1127280.1203655607402751No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1087990.11617036266926754No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTTTGGAATTCTCGGGTGCCAAG1066580.11388430538496436No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGGAATTCTCGGGTGCCAA1046240.11171249757727045No Hit
CCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCTGGAATTCTCGGGTG1025470.10949477642850927No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAACTGGAATTCTCGGGTGCCAA1024230.10936237516589666No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG1015270.10840566926830876No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCAA1004090.1072119224005596No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1002940.1070891309070076No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC978290.10445712193652308No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC959730.10247537400580532No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC959410.10244120593803432No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTGGAATTCTCGG939810.10034841178706085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)