FastQCFastQC Report
Tue 25 Jul 2023
SRR10491340.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10491340.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46228496
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTAT5664581.2253437793001096No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAA3380460.7312502660696554No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC2763100.597704930742285No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2577580.5575738393046574Illumina PCR Primer Index 6 (96% over 29bp)
ATCCGAGTCACGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT2364590.5115005255632803RNA PCR Primer, Index 6 (95% over 24bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA2151250.4653515009443526No Hit
TCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTATG2093440.4528462271409392No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCA2036910.44061783883256767No Hit
TAGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA1969780.4260964925183809No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCAC1660380.35916807676373463No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAT1632000.3530290061783537No Hit
TCCCCGGCATCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1617280.3498448229853725Illumina PCR Primer Index 6 (95% over 24bp)
TCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTAT1579100.3415858478285774No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1517220.3282001646776482No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGCCTGTAGGCACCATCA1480920.3203478650916958No Hit
TCGGCGGCGGCGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1372060.2967996190055588RNA PCR Primer, Index 6 (95% over 24bp)
AAATGGATTTTTGGAGCAGGGCTGTAGGCACCATCAATATCTCGTATGCC1346020.29116672971580126No Hit
TCCGCGCGGCGCCTCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTC1318630.28524181275549176No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA1282160.27735273931472915No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATC1215640.2629633462442732No Hit
TTCCCGTGGATCGCCCCAGCCTGTAGGCACCATCAATATCTCGTATGCCG1189370.25728070409212533No Hit
TCCCGGGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1180120.255279773756862TruSeq Adapter, Index 6 (95% over 24bp)
ATCCCACTTCTGACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1168650.25279862014113547Illumina PCR Primer Index 6 (95% over 24bp)
ACCCCGTCCGTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1164370.25187278426708926Illumina PCR Primer Index 6 (95% over 24bp)
ATCCCACCGCTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1160770.2510940438122841Illumina PCR Primer Index 6 (95% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATC1128410.24409403239075744No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATATCTCGTATGCC1122760.24287184251029925No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1118820.24201955434587358Illumina PCR Primer Index 6 (95% over 24bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1109400.2399818501558Illumina PCR Primer Index 6 (100% over 28bp)
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATATCTCG1102000.23838110588758932No Hit
AGAGCACTGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT1089020.2355733139144306Illumina PCR Primer Index 6 (100% over 27bp)
ACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGCTGTAGGCACCATCAAT1087570.23525965456457854No Hit
ATCCCAGCGGTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1080240.23367405247187797Illumina PCR Primer Index 6 (95% over 24bp)
TCCCCGTACGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1073980.23231990934768892Illumina PCR Primer Index 6 (95% over 24bp)
TCCTCCCGACCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG1040500.22507762311800064Illumina PCR Primer Index 6 (96% over 25bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCTGTAGGCACCATC1036090.22412366606086426No Hit
ATCCCAGCGGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT988000.21372099148542495Illumina PCR Primer Index 6 (95% over 24bp)
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATATC854530.18484918912352244No Hit
ACCTGCGGAAGGATCATTAACGGAGCCCTGTAGGCACCATCAATATCTCG847790.1833912139386927No Hit
ATGGATTTTTGGAGCAGGGCTGTAGGCACCATCAATATCTCGTATGCCGT843000.1823550565002158No Hit
TTCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTA829180.17936555842093588No Hit
GGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTATGCC805880.17432537714400226No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATATC802060.17349904699473676No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT781580.16906887907406723Illumina PCR Primer Index 6 (95% over 24bp)
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCCTGTAGGCACCATCA780300.16879199357902536No Hit
AGGGGGACGGCGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG776580.16798729510906TruSeq Adapter, Index 6 (96% over 25bp)
AACACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAAT772620.1671306806087743No Hit
ATCCTGCCGACTACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT766880.165889022216946Illumina PCR Primer Index 6 (95% over 24bp)
ACCTGCGGAAGGATCATTAACGGAGCCTGTAGGCACCATCAATATCTCGT765940.16568568443152465No Hit
ACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATATCTCGTATGCC754760.16326726268576852No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATATC752320.16273944971084503No Hit
CGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCCTGTAG748060.16181794017265888No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGCTGTAGGCACCATCAAT742620.16064117681873102No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGCCTGTAGGCACCATCAA728770.15764518923566104No Hit
TGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTATGC724890.15680588007881546No Hit
ACGCGGGAGACCCGGGTTCATTTCCCGGCCAATGCCTGTAGGCACCATCA712370.15409759383043742No Hit
ACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGCTGTA687980.14882162724913223No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGCTGTAGGCACCA682900.1477227379406849No Hit
TTCCCGGCCCATGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT673120.1456071597051308TruSeq Adapter, Index 6 (95% over 24bp)
TCCGCGCGGCGCCTCGCCTCGGCCGGCGCTGTAGGCACCATCAATATCTC669560.14483707192204567No Hit
TTCCCGGCCAATGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT657780.14228886010048866TruSeq Adapter, Index 6 (95% over 24bp)
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATA636920.1377764917984786No Hit
ACCCCACTCCTGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG636330.13764886489060774Illumina PCR Primer Index 6 (100% over 24bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGCTGTAGGCACCATCAA615800.1332078811302881No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGCTGTAGGCACCAT614810.1329937275052167No Hit
TCCTCACACGGGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT611820.13234694029414237Illumina PCR Primer Index 6 (100% over 23bp)
TTCCCGGCCAACGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT594000.12849217504285668Illumina PCR Primer Index 6 (100% over 23bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA579720.1254031712387961No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGCTGTAGGCACCATCAATATC578610.1251630595985645No Hit
TAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC568760.12303233918750028No Hit
GCCTCACCTGGAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT565780.122387715144356Illumina PCR Primer Index 6 (100% over 23bp)
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCCTGTAGGCACCATCAA560820.12131478385106884No Hit
TTCCCGTGGATCGCCCCAGCTGCTGTAGGCACCATCAATATCTCGTATGC559790.12109197755427734No Hit
ACTGCATAATTTGTGGTAGTGGGGGACTGCGCTGTAGGCACCATCAATAT557510.12059877526623405No Hit
ATCTCGGTGGAACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT553800.11979623996419872Illumina PCR Primer Index 6 (95% over 24bp)
CTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTAT540530.11692571612106958No Hit
CCGCAGCAACCTCGGTTCGTATCCGAGTCACGGCCTGTAGGCACCATCAA537460.11626162356655513No Hit
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAAT525010.11356847949368719No Hit
AGGGGGACGGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC520520.1125972170931107Illumina PCR Primer Index 6 (96% over 26bp)
TCCTGCGGAAGGATCATTAACGGAGCCCTGTAGGCACCATCAATATCTCG518470.11215376766745776No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCCTGTAGGCACCATCAATAT517070.11185092415725573No Hit
TCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTA512120.11078015603189859No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGCTGTAGGCACCATCAAT510810.1104967810330667No Hit
ATCCCACTCCTGACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT508350.10996464172228315Illumina PCR Primer Index 6 (95% over 24bp)
GGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTATGC499410.10803076959285027No Hit
TGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATATCTCGTATGCCG489720.10593465986866628No Hit
GCCTCACACGGGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT489320.10584813315146571TruSeq Adapter, Index 6 (95% over 24bp)
ATCACGTCGGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC487420.10543713124476298RNA PCR Primer, Index 6 (96% over 26bp)
TCCTGCGGAAGGATCATTAACGGAGCCTGTAGGCACCATCAATATCTCGT485900.10510832971940079No Hit
ACGCGCCCCGCGCGCGCGGGAGGGCGCGCTGTAGGCACCATCAATATCTC478360.10347730110016991No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)