FastQCFastQC Report
Tue 18 Jul 2023
SRR10491341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10491341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54124235
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTAT7794451.4401034952272305No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCA3820440.7058649420171943No Hit
TAGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA3333240.6158498129350004No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3325020.6143310847719141No Hit
TCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTATG3181190.5877570371202475No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC3177940.5871565667394653No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCAC3089560.5708274675845303No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA2751100.5082935583292771No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT2742910.5067803729697057RNA PCR Primer, Index 6 (95% over 24bp)
AAATGGATTTTTGGAGCAGGGCTGTAGGCACCATCAATATCTCGTATGCC2632110.4863089519879588No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA2581630.4769822612735311No Hit
TCGGCGGCGGCGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT2355760.435250493609748RNA PCR Primer, Index 6 (95% over 24bp)
TTCCCGTGGATCGCCCCAGCCTGTAGGCACCATCAATATCTCGTATGCCG2333890.4312097898473761No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2070820.38260494582510035Illumina PCR Primer Index 6 (96% over 29bp)
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAA2055910.3798501724781884No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT2039910.37689401060356786Illumina PCR Primer Index 6 (95% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAT1787260.3302143670021387No Hit
ATGGATTTTTGGAGCAGGGCTGTAGGCACCATCAATATCTCGTATGCCGT1775020.32795290316805403No Hit
ATCCCACCGCTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1732860.320163416628429Illumina PCR Primer Index 6 (95% over 24bp)
TCCGCGCGGCGCCTCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTC1709100.3157735162446176No Hit
TCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTAT1614050.29821206710820025No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATATCTCG1610390.2975358450793808No Hit
ACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATATCTCGTATGCC1595320.29475151011372264No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATC1556450.28756988435956643No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATATCTCGTATGCC1453250.2685026402682643No Hit
ACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGCTGTA1451460.26817191965854115No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1430200.26424391956763915Illumina PCR Primer Index 6 (95% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATC1406490.25986325718968595No Hit
ATCCCAGCGGTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1387650.25638237658232027Illumina PCR Primer Index 6 (95% over 24bp)
TCCCCGGCATCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1386760.25621794007804455Illumina PCR Primer Index 6 (95% over 24bp)
AGGGGGACGGCGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG1349030.24924694085745508TruSeq Adapter, Index 6 (96% over 25bp)
ATCCCAGCGGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1268270.23432571379530814Illumina PCR Primer Index 6 (95% over 24bp)
ACGCGCCCCGCGCGCGCGGGAGGGCGCGCTGTAGGCACCATCAATATCTC1228810.22703507957202534No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATATC1222990.22595977569013215No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAAT1214330.22435975307549383No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGCCTGTAGGCACCATCA1209150.22340269566858542No Hit
ACCCCGTCCGTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1130210.2088177320196766Illumina PCR Primer Index 6 (95% over 24bp)
AGAGCACTGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT1129800.20874198037163944Illumina PCR Primer Index 6 (100% over 27bp)
TCCCGGGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1106680.20447032646281282TruSeq Adapter, Index 6 (95% over 24bp)
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATATC1089870.20136450889328966No Hit
TGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTATGC1067470.19722588226882098No Hit
TTCCCGTGGATCGCCCCAGCTGCTGTAGGCACCATCAATATCTCGTATGC1061330.19609145514943538No Hit
AGGGGGACGGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC1035200.19126367328794577Illumina PCR Primer Index 6 (96% over 26bp)
ATCTCGGTGGAACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1022010.1888266873425555Illumina PCR Primer Index 6 (95% over 24bp)
ATCCCACTTCTGACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1000610.1848728208352506Illumina PCR Primer Index 6 (95% over 24bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGCTGTAGGCACCA982240.18147877748295196No Hit
TTCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTA965260.17834155069351096No Hit
ACCTGCGGAAGGATCATTAACGGAGCCCTGTAGGCACCATCAATATCTCG957470.1769022693808051No Hit
ATCCCGGACGAGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT956370.17669903325192496Illumina PCR Primer Index 6 (95% over 24bp)
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAAT946990.17496598335292868No Hit
TCCCCGTACGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT926490.17117840095107117Illumina PCR Primer Index 6 (95% over 24bp)
ACCTGCGGAAGGATCATTAACGGAGCCTGTAGGCACCATCAATATCTCGT900180.16631736226849209No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGCTGTAGGCACCAT892750.16494459459796523No Hit
TCCGCGCGGCGCCTCGCCTCGGCCGGCGCTGTAGGCACCATCAATATCTC838180.1548622350043377No Hit
ACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGCTGTAGGCACCATCAAT836790.15460541844148004No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCGACGGGGGGGGTGCTGTAGGC823680.1521832133054629No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC805080.14874667512621656No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATATC800470.14789493098609155No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC782450.1445655536748002No Hit
TAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC781920.14446763081270342No Hit
GGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTATGCC779190.14396323569284628No Hit
ATCCTGCCGACTACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT764790.14130269000568785Illumina PCR Primer Index 6 (95% over 24bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGCTGTAGGCACCATCAA754760.13944954603053514No Hit
ACCCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATA737020.13617190155204964No Hit
TTCCCGGCCAATGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT712330.1316101742592759TruSeq Adapter, Index 6 (95% over 24bp)
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCCTGTAGGCACCATCA706790.13058660321018856No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCTGTAGGCACCATC706120.13046281393168882No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGCTGTAGGCACCATCAAT700580.12943924288260147No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCTGTAGGCACC696730.12872791643152093No Hit
TTCCCGGCCCATGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT693860.1281976548952609TruSeq Adapter, Index 6 (95% over 24bp)
TCCTCCCGACCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG683220.12623180724863825Illumina PCR Primer Index 6 (96% over 25bp)
CGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCCTGTAG682540.12610617036896687No Hit
CTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTAT665430.1229449247642946No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCA664890.12284515430102615No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGCTGTAGGCACCATCAATATC655170.1210492859621942No Hit
ACTGCATAATTTGTGGTAGTGGGGGACTGCGCTGTAGGCACCATCAATAT653310.12070563214426958No Hit
TAACCGTGCAAAGGTAGCACTGTAGGCACCATCAATATCTCGTATGCCGT649430.11998876288967411No Hit
GGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTATGC636380.11757764336068675No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATATCTCGTATGCC634390.11720997072753084No Hit
TCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTA633840.11710835266309076No Hit
TTCGCCCGTCACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATAT632480.11685707890374802No Hit
TTTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTA628740.11616607606555547No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA592380.10944819820548041No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC589650.10894380308562328No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTCTGTAGGCACCATCA588990.1088218614082952No Hit
TCCTCACACGGGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT586200.10830638068140826Illumina PCR Primer Index 6 (100% over 23bp)
ACCTCAGAGGGGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT585920.10825464784860238TruSeq Adapter, Index 6 (95% over 24bp)
TTCCCCCGCGAGGGGGGCCCGGGCCTGTAGGCACCATCAATATCTCGTAT581670.10746941735065632No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA581450.1074287701248803No Hit
ATCACGTCGGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC581160.10737518969090279RNA PCR Primer, Index 6 (96% over 26bp)
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGCTGTAGGCACCATCAAT580910.10732899966161184No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATATCTCGTATGC570040.10532065718804155No Hit
TCCTGCGGAAGGATCATTAACGGAGCCCTGTAGGCACCATCAATATCTCG568870.10510448785095991No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCAC564510.10429893374012585No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATATCTCGTATGC560430.10354511246209762No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA558490.10318667783479989No Hit
TTCCCGGCCAACGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT554670.10248089418723423Illumina PCR Primer Index 6 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)