FastQCFastQC Report
Tue 25 Jul 2023
SRR10491342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10491342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49450892
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGAGTCACGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT4919300.9947848867923353RNA PCR Primer, Index 6 (95% over 24bp)
ATCCCACCGCTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT4087900.8266584958669705Illumina PCR Primer Index 6 (95% over 24bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA3153310.6376649383796757No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAA2994590.6055684496045086No Hit
TTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTAT2851280.5765881836873641No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCAC2844890.575295992638515No Hit
TCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTATG2470360.4995582283935343No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAT2457500.49695766863012303No Hit
TCCCCGGCATCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT2346460.47450306862007663Illumina PCR Primer Index 6 (95% over 24bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2183100.4414682752335387No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT2159850.4367666411356139Illumina PCR Primer Index 6 (95% over 24bp)
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATATCTCGTATGCC2105270.4257294287027219No Hit
TAGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA2039750.41247992048353743No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2012230.4069148034781657Illumina PCR Primer Index 6 (96% over 29bp)
ATCCCGGACGAGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1880510.380278276881234Illumina PCR Primer Index 6 (95% over 24bp)
TTCCCGGCCAATGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1835290.3711338513367969TruSeq Adapter, Index 6 (95% over 24bp)
TTCCCGGCCCATGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1781940.36034537051424675TruSeq Adapter, Index 6 (95% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATC1736660.3511888117205247No Hit
TTCCCGTGGATCGCCCCAGCCTGTAGGCACCATCAATATCTCGTATGCCG1674980.3387158314555782No Hit
ATCCCAGCGGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1607760.3251225478399864Illumina PCR Primer Index 6 (95% over 24bp)
TCCGCGCGGCGCCTCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTC1572820.31805695233970704No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGCTGTAGGCACCATCAATATC1530670.30953334471701743No Hit
ACCCCGTCCGTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1485420.3003828525479379Illumina PCR Primer Index 6 (95% over 24bp)
AGAGCACTGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT1483670.30002896611045965Illumina PCR Primer Index 6 (100% over 27bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGCTGTAGGCACCATCAA1478260.29893495146659843No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATC1431440.2894669726078955No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1420460.28724658798874647Illumina PCR Primer Index 6 (95% over 24bp)
ATCCCAGCGGTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1398690.2828442407065175Illumina PCR Primer Index 6 (95% over 24bp)
TCCCCGTACGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1330340.2690224475627255Illumina PCR Primer Index 6 (95% over 24bp)
ACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATATCTCGTATGCC1310820.2650750971286827No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCA1233520.2494434276332164No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA1188640.24036775716806077No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATATCTCGTATGCC1128430.22819204151059602No Hit
ATCTCGGTGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT1091840.22079278165498006RNA PCR Primer, Index 6 (96% over 27bp)
GCCCACCCAGGGACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1090560.2205339390035674Illumina PCR Primer Index 6 (95% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATATC1089650.22034991805607876No Hit
TCGGCGGCGGCGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1088860.22019016360715998RNA PCR Primer, Index 6 (95% over 24bp)
ATCCGAGTCACGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG1073910.21716696232698895Illumina PCR Primer Index 6 (100% over 24bp)
TCCCGGGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1049810.21229344053086038TruSeq Adapter, Index 6 (95% over 24bp)
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGCCTGTAGGCACCATCA1031060.20850180012930808No Hit
ATCCCAGCGGTGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG1002940.2028153506310867Illumina PCR Primer Index 6 (100% over 24bp)
ATGGATTTTTGGAGCAGGGCTGTAGGCACCATCAATATCTCGTATGCCGT979440.19806316132780782No Hit
TCCTCCCGACCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG953510.1928195754284877Illumina PCR Primer Index 6 (96% over 25bp)
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATATCTCGTATGC931450.18835858410804804No Hit
AAATGGATTTTTGGAGCAGGGCTGTAGGCACCATCAATATCTCGTATGCC912450.1845163885011417No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC859650.1738391291303704No Hit
ACGCCCGGGGCACCCCCCTCGCGGCTGTAGGCACCATCAATATCTCGTAT839750.16981493478418955No Hit
ATCCCACTTCTGACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT825810.16699597653364878Illumina PCR Primer Index 6 (95% over 24bp)
CGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCCTGTAG790640.15988387024444373No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATATCTCG784790.1587008784391594No Hit
TTCCCGGCCAACGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT772230.1561609849221729Illumina PCR Primer Index 6 (100% over 23bp)
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATATCTCGTATGC766250.15495170441010447No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATATC766080.15491732687046372No Hit
AGGGGGACGGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC762150.15412259904229836Illumina PCR Primer Index 6 (96% over 26bp)
GGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTATGCC761840.15405991058765936No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATATC759100.1535058255369792No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC729750.14757064442841597No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC710750.14372844882150962No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGGCACCATCAA693310.14020171769601245No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA692510.14003994103887954Illumina PCR Primer Index 6 (100% over 28bp)
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA675690.13663858682266034No Hit
ATCTCGGTGGAACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT669980.13548390593237428Illumina PCR Primer Index 6 (95% over 24bp)
ATCACGTCGGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC667030.13488735450919673RNA PCR Primer, Index 6 (96% over 26bp)
ACCTGCGGAAGGATCATTAACGGAGCCTGTAGGCACCATCAATATCTCGT665780.13463457848242655No Hit
TTCTCGCTGGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT664710.1344182022035113Illumina PCR Primer Index 6 (95% over 24bp)
ACCTGCGGAAGGATCATTAACGGAGCCCTGTAGGCACCATCAATATCTCG664680.13441213557886883No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCTGTAGGCACCAT662020.13387422819390193No Hit
GCCCCACGTTGGGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC661320.13373267361891067Illumina PCR Primer Index 6 (95% over 23bp)
AGGGGGACGGCGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG657190.13289750162646208TruSeq Adapter, Index 6 (96% over 25bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA645420.1305173625583943No Hit
ATCCCAGCGGGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG645220.13047691839411107TruSeq Adapter, Index 6 (96% over 25bp)
CGCGCGGCGCCTCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT636530.12871961945600496Illumina PCR Primer Index 6 (95% over 22bp)
GCCTCAGAGAGGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT628800.12715645250645832Illumina PCR Primer Index 6 (100% over 23bp)
TCCCCCGCGAGGGGGGCCCGGGCCTGTAGGCACCATCAATATCTCGTATG592800.11987650293547789No Hit
ATCCTGCCGACTACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT592640.11984414760405132Illumina PCR Primer Index 6 (95% over 24bp)
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAAT590980.11950846104050056No Hit
TTCCCGTGGATCGCCCCAGCTGCTGTAGGCACCATCAATATCTCGTATGC585250.11834973573378615No Hit
ACCCCGTCCGTGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG580400.11736896474991795TruSeq Adapter, Index 6 (96% over 25bp)
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCCTGTAGGCACCATCA580210.1173305427938489No Hit
GCCTCACCTGGAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG576930.11666725849960402Illumina PCR Primer Index 6 (96% over 25bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCTGTAGGCACCATC575740.11642661572211883No Hit
TGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTATGC571780.11562582126931097No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCTGTAGGCACC571560.11558133268859944No Hit
TTTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATCTC565360.11432756359581946No Hit
ATCCCACCACTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT564630.11417994239618569Illumina PCR Primer Index 6 (95% over 24bp)
ACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGCTGTA558230.1128857291391225No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGCTGTAGGCACCAT553440.11191709140453929No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTGTAGGCACCATCAATAT553280.1118847360731127No Hit
AACCATCCTCTGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG546130.11043885719998742TruSeq Adapter, Index 6 (96% over 25bp)
ACGCGCCCCGCGCGCGCGGGAGGGCGCGCTGTAGGCACCATCAATATCTC540680.10933675372326954No Hit
ATCTCGCTGGGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG539930.10918508810720745TruSeq Adapter, Index 6 (96% over 25bp)
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGCCTGTAGGCACCATCAA539130.10902331145007456No Hit
TCGACTCCCGGTATGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT534090.10800411851013729TruSeq Adapter, Index 6 (95% over 21bp)
GCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGGCTGTAGGCACCA517080.10456434233784902No Hit
ACGCGGGAGACCCGGGTTCATTTCCCGGCCAATGCCTGTAGGCACCATCA513280.10379590321646776No Hit
GCCTCACACGGGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT496200.10034197158668037TruSeq Adapter, Index 6 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)