FastQCFastQC Report
Tue 25 Jul 2023
SRR10491343.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10491343.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71155275
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTAT4957690.6967424410909803No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAA4520700.6353288635311999No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT4483820.6301458324769316RNA PCR Primer, Index 6 (95% over 24bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA4170260.5860788254981798No Hit
ATCCCACCGCTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT4105080.57691857701344Illumina PCR Primer Index 6 (95% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAT3604060.5065063693450697No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCAC3576400.5026190960543684No Hit
TCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATATCTCGTATG3419380.4805518635125786No Hit
TAGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA3394220.47701593451785546No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3053450.42912489622167854No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCA2955010.41529036322324664No Hit
TCCTCCCGACCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG2602570.36575924975344415Illumina PCR Primer Index 6 (96% over 25bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATC2541250.3571414768616944No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2490300.3499810801096616Illumina PCR Primer Index 6 (96% over 29bp)
TCCCCGGCATCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT2425450.3408672090719908Illumina PCR Primer Index 6 (95% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATC2424960.34079834558997907No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT2386740.3354269939930666Illumina PCR Primer Index 6 (95% over 24bp)
ATCCCGGACGAGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT2232430.31374061866811703Illumina PCR Primer Index 6 (95% over 24bp)
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATATCTCGTATGCC2224340.312603668526332No Hit
TTCCCGGCCCATGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT2073700.29143306662787827TruSeq Adapter, Index 6 (95% over 24bp)
TTCCCGTGGATCGCCCCAGCCTGTAGGCACCATCAATATCTCGTATGCCG2048800.2879336774399368No Hit
TTCCCGGCCAATGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1970830.2769759515369732TruSeq Adapter, Index 6 (95% over 24bp)
ATCCCAGCGGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1969470.27678482023996115Illumina PCR Primer Index 6 (95% over 24bp)
TCCGCGCGGCGCCTCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTC1966590.2763800716109944No Hit
ATCCCAGCGGTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1895650.2664103258683211Illumina PCR Primer Index 6 (95% over 24bp)
ACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATATCTCGTATGCC1867390.2624387299465851No Hit
TCCCCGTACGGGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1807340.2539994399571922Illumina PCR Primer Index 6 (95% over 24bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC1789890.2515470567712654No Hit
AAATGGATTTTTGGAGCAGGGCTGTAGGCACCATCAATATCTCGTATGCC1779770.2501248150611462No Hit
ACCCCGTCCGTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1745200.24526642613636163Illumina PCR Primer Index 6 (95% over 24bp)
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGCCTGTAGGCACCATCA1727160.24273112569658398No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1712270.24063851906973868Illumina PCR Primer Index 6 (95% over 24bp)
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATATCTCGTATGCC1697740.23859650602151422No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGCTGTAGGCACCATCAA1683770.23663319409558883No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATATC1653870.23243111631569127No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA1639510.2304129946795933No Hit
ATGGATTTTTGGAGCAGGGCTGTAGGCACCATCAATATCTCGTATGCCGT1624660.2283260095614837No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATATCTCG1560090.21925148908496242No Hit
TCCCGGGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1454330.2043882199879067TruSeq Adapter, Index 6 (95% over 24bp)
ATCCCACTTCTGACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1447220.20338899681014516Illumina PCR Primer Index 6 (95% over 24bp)
AGAGCACTGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT1414840.19883838548863733Illumina PCR Primer Index 6 (100% over 27bp)
TCGGCGGCGGCGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1385830.194761386278108RNA PCR Primer, Index 6 (95% over 24bp)
GCCCACCCAGGGACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1345740.1891272291478039Illumina PCR Primer Index 6 (95% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGCTGTAGGCACCATCAATATC1315130.18482536958784854No Hit
GGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTATGCC1267440.17812312579777112No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATATC1252200.17598133096948892No Hit
TTCCCGGCCAACGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1240800.17437920097982895Illumina PCR Primer Index 6 (100% over 23bp)
ATCCTGCCGACTACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT1134740.15947377056725592Illumina PCR Primer Index 6 (95% over 24bp)
CGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCCTGTAG1115580.1567810678828801No Hit
TGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTATGC1095130.15390707154177957No Hit
AGGGGGACGGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC1069850.15035427802084947Illumina PCR Primer Index 6 (96% over 26bp)
TCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTAT1068660.15018703813596393No Hit
ACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGCTGTA1063940.14952370010515734No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC1061650.14920186873004143No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATATC1014290.14254600238703313No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCTGTAGGCACCATC983230.13818090085380177No Hit
ATCTCGGTGGAACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT966950.13589294679839267Illumina PCR Primer Index 6 (95% over 24bp)
ACCTGCGGAAGGATCATTAACGGAGCCCTGTAGGCACCATCAATATCTCG965570.1356990047470128No Hit
ACGCGCCCCGCGCGCGCGGGAGGGCGCGCTGTAGGCACCATCAATATCTC951350.13370055839148962No Hit
AGGGGGACGGCGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG945980.13294587084372872TruSeq Adapter, Index 6 (96% over 25bp)
ATCACGTCGGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC939460.13202956491981796RNA PCR Primer, Index 6 (96% over 26bp)
ACCTGCGGAAGGATCATTAACGGAGCCTGTAGGCACCATCAATATCTCGT935820.13151800762487392No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGCTGTAGGCACCATCAATA924680.12995241744199568No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATATCTCGTATGC922670.12966993662802934No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCCTGTAGGCACCATCA896120.12593866020474237No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC895550.1258585537052594No Hit
ATCCGAGTCACGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG894520.12571379985531642Illumina PCR Primer Index 6 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGCTGTAGGCACCAT885010.1243772861534159No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATATCTCGTATGC877810.12336541458099909No Hit
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAAT873350.12273861635697424No Hit
CGCGCGGCGCCTCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT871330.12245473016582398Illumina PCR Primer Index 6 (95% over 22bp)
TGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATATCTCGTATGCCG862820.12125875418231467No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA861930.12113367561294647No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGCCTGTAGGCACCATCAA843680.11856886225230666No Hit
ATCCCAGCGGTGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG823780.11577216165632133Illumina PCR Primer Index 6 (100% over 24bp)
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATA820250.1152760635104003No Hit
ACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGCTGTAGGCACCATCAAT819420.11515941720413561No Hit
TTTGCCAAGAATGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATA818660.11505260853815827No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA807860.11353480117953307No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGGCACCATCAA803520.11292486748171517No Hit
TCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCA794430.11164737962153896No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA784620.11026870460412105Illumina PCR Primer Index 6 (100% over 28bp)
ATCTCGGTGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT780660.1097121752392918RNA PCR Primer, Index 6 (96% over 27bp)
ACGCGGGAGACCCGGGTTCATTTCCCGGCCAATGCCTGTAGGCACCATCA773560.10871435743871415No Hit
GCCTCAGAGAGGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT769270.10811145062681578Illumina PCR Primer Index 6 (100% over 23bp)
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGCTGTAGGCACCATCAAT757750.1064924561109489No Hit
TTCCCGTGGATCGCCCCAGCTGCTGTAGGCACCATCAATATCTCGTATGC755400.10616219247272954No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCTGTAGGCACCAT750940.10553539424870467No Hit
GCCCCACGTTGGGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC746250.10487627234944984Illumina PCR Primer Index 6 (95% over 23bp)
TCCCCCGCGAGGGGGGCCCGGGCCTGTAGGCACCATCAATATCTCGTATG734510.1032263595355369No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCGACGGGGGGGGTGCTGTAGGC734170.10317857671128389No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAAT726730.10213297608645318No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)