FastQCFastQC Report
Tue 18 Jul 2023
SRR10493408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10493408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38553578
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length40-86
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA9697662.5153722437901873No Hit
TCAGTAGCGAATGGGTGAGCAACACGTACCTAACCTACCTTTAAGACTGG5377041.3946928609323888No Hit
TTACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAAT4058821.0527738826212187No Hit
AAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCC3366970.8733223152465901No Hit
TCCACGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAAT3262280.8461678965309005No Hit
TACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAAT2956680.7669015830385445No Hit
ATTTCACTAACGCAGCTAACGCGTTAAATGATCCGCCTGAGTAG2917230.7566690697294036No Hit
TCAACATTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTGGTTACC2540170.6588675115964593No Hit
CAGTAGCGAATGGGTGAGCAACACGTACCTAACCTACCTTTAAGACTGGG2414690.6263205972737472No Hit
TGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG2311900.599658999224404No Hit
ATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCG2173870.5638568747108245No Hit
ATTTCACTAACGCAGCTAACGCGTTAAATGATCCGCCTGAGTA1819140.47184725630394153No Hit
TCACGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATT1791010.4645509166490332No Hit
TATGATAGTAGAAGAAGTTGGAAAGCTTCAACATAGAAGGTGATATTCCT1746450.4529929751267185No Hit
ACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAAT1629750.4227234110411231No Hit
TCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGG1523540.39517473579235624No Hit
AGTAGCGAATGGGTGAGCAACACGTACCTAACCTACCTTTAAGACTGGG1516050.3932319848497589No Hit
ACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGAAGG1462750.3794070682622505No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1350140.3501983655057904No Hit
ATCCTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATCTGCCGGGA1338770.347249222886654No Hit
TTCAGTAGCGAATGGGTGAGCAACACGTACCTAACCTACCTTTAAGACTG1245900.32316066747423544No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG1207880.3132990665613449No Hit
ATGATAGTAGAAGAAGTTGGAAAGCTTCAACATAGAAGGTGATATTCCTG1174510.30464357938451264No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT1109140.2876879546692138No Hit
ACGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGT1093850.28372204520161526No Hit
CCACGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATT1090470.28284534317411475No Hit
CTAATACCGGATATAGTTATTTATCGCATGATGAGTAATAGAAAGGAGCT1081250.28045386604584405No Hit
AGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGC1059250.27474752148814824No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG1057230.27422357530603253No Hit
ACGTCCACGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAA1047400.27167387680593486No Hit
TTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGC1036380.2688155169411254No Hit
AATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCC1034800.26840569765016364No Hit
TTTTTTGAGAGTTTGATCCTAGCTCAGGATGAACGCTCGCTGTGTGCCTA1021320.2649092647120846No Hit
GTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT990910.2570215402575605No Hit
TCGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGT987640.25617336995284845No Hit
GAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGC986500.2558776775530406No Hit
ATTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTGGTTACCCGCAT970990.2518547046398651No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCG929750.24115790238716625No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT896620.23256466624187255No Hit
TGAAATTCCTTGTAGACTACGAGGTTGATAGGTTGGAAGTGTA894560.23203034488783375No Hit
TTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT885110.22957921052100533No Hit
AGTAGCGAATGGGTGAGCAACACGTACCTAACCTACCTTTAAGACTGG884300.2293691132895629No Hit
TTATGATAGTAGAAGAAGTTGGAAAGCTTCAACATAGAAGGTGATATTCC860630.2232296053040784No Hit
TCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG846620.21959570133801848No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG840300.21795642417417133No Hit
TTACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAATA836940.21708490973263236No Hit
CAGTAGCGAATGGGTGAGCAACACGTACCTAACCTACCTTTAAGACTGG807050.20933206251310837No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGG774590.20091261049752632No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA752330.19513882732233048No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC734440.19049853167973152No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA727220.18862581314761498No Hit
AAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCC724690.18796958352347998No Hit
GAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCC716030.18572335880213245No Hit
TAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGC714150.18523572572174754No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGT713260.18500487814645894No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA711460.1845379954099202No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA709460.18401923681376603No Hit
TTTTTTTGAGAGTTTGATCCTAGCTCAGGATGAACGCTCGCTGTGTGCCT705740.1830543458249193No Hit
ACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGAA700500.18169519830299538No Hit
TCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTG677690.17577875651385716No Hit
TCGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTG673780.17476458345837578No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC673210.17461673725847185No Hit
AAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGC667790.17321090146289406No Hit
TCCTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATCTGCCGGGA629140.16318589159221486No Hit
AAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCG628560.16303545159933017No Hit
TTACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAA625530.1622495323261566No Hit
CCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGC624530.1619901530280795No Hit
TACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAATA619440.1606699124008672No Hit
CTCAACATTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTGGTTAC600420.1557365181514411No Hit
ACGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTG595880.15455893613817115No Hit
TTGAAATTCCTTGTAGACTACGAGGTTGATAGGTTGGAAGTGTA589800.1529819100058625No Hit
TCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGT581590.15085240596864966No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT579170.15022470806730312No Hit
TTTAGTATATTGGCATATACCCGAAACCAGGTGATCTATCCATGAGC578840.1501391128989377No Hit
ACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCAGGATGAAGTTCT568820.14754013233220534No Hit
TGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCG558650.14490224487076142No Hit
ATACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAAT542570.14073142575768194No Hit
TCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGC532790.1381946962224881No Hit
GCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACC523510.13578765633633277No Hit
TCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG515410.13368668402190842No Hit
TTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGG502290.1302836276311371No Hit
ATTTCACTAACGCAGCTAACGCGTTAAATGATCCGCCTGAGT495200.1284446284077706No Hit
TAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCC466700.12105231841257379No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCC460320.11939747849084202No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAG456840.11849483853353378No Hit
TACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAA456050.11828992888805287No Hit
AGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG455980.11827177233718748No Hit
GGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGAC450140.11675699723641733No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCTCCGCCGAGGGCGCACCACC435460.11294930914064578No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTC434870.1127962753547803No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA432320.11213485814468374No Hit
AGGATGAACGCTCGCTGTGTGCCTAATACATGCATGTTGAACGGG432140.11208816987102986No Hit
TTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTGGTTACCCGCATC429470.11139562714516406No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCG427870.11098062026824074No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC422800.10966556722698992No Hit
AGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCT413350.10721443286016152No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC411780.10680720736218051No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG405610.1052068370930449No Hit
TTCAACATTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTGGTTAC402540.10441054264794826No Hit
TTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCC401260.1040785371464096No Hit
ATCCTAGCTCAGGATGAACGCTCGCTGTGTGCCTAATACATGCATGTTGA395220.10251188618602403No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC393930.1021772868915046No Hit
TCGAACCTGGAGATAGCTGGTTCTCTTCGAAATAGCTTTAGGGCTAGCGT392470.10179859311631206No Hit
ATACCCGGCCGTCGCCGGCAGTCGAGAGTGGACGGGAGCGGCGGGGGCGG390160.10119942693775401No Hit
TTTAGTATATTGGCATATACCCGAAACCAGGTGATCTATCCATGAGCAGG385550.10000368837361866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)