FastQCFastQC Report
Tue 18 Jul 2023
SRR10493409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10493409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33095616
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length40-86
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA8071062.4387097070500214No Hit
TCAGTAGCGAATGGGTGAGCAACACGTACCTAACCTACCTTTAAGACTGG4317291.3044899964998384No Hit
TTACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAAT4089911.2357860328086958No Hit
AAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCC3352641.0130163463342092No Hit
TCCACGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAAT3124610.9441159820080097No Hit
TCAACATTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTGGTTACC3051580.9220496152723069No Hit
TACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAAT2930980.8856097435986688No Hit
ATTTCACTAACGCAGCTAACGCGTTAAATGATCCGCCTGAGTAG2452150.7409289496228141No Hit
TGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG2260370.6829816976363274No Hit
CAGTAGCGAATGGGTGAGCAACACGTACCTAACCTACCTTTAAGACTGGG1921310.5805330832941741No Hit
TATGATAGTAGAAGAAGTTGGAAAGCTTCAACATAGAAGGTGATATTCCT1850150.5590317460777886No Hit
ATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCG1696300.5125452265339313No Hit
TCACGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATT1645110.4970779211361408No Hit
ACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAAT1613810.48762047517109214No Hit
ATTTCACTAACGCAGCTAACGCGTTAAATGATCCGCCTGAGTA1451430.4385565749856416No Hit
AGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGC1277240.3859242263386184No Hit
AGTAGCGAATGGGTGAGCAACACGTACCTAACCTACCTTTAAGACTGGG1244530.3760407420729078No Hit
ATCCTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATCTGCCGGGA1219090.36835392337160305No Hit
ATTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTGGTTACCCGCAT1215990.36741724341979315No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1177550.35580241201734997No Hit
AATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCC1097030.33147290565614496No Hit
TCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGG1090580.3295240070467339No Hit
ACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGAAGG1089620.329233938416496No Hit
ACGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGT1047950.31664314693523155No Hit
ACGTCCACGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAA1025020.30971473684006967No Hit
CCACGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATT1024180.30946092678861153No Hit
GTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT1015190.3067445549283627No Hit
ATGATAGTAGAAGAAGTTGGAAAGCTTCAACATAGAAGGTGATATTCCTG998900.3018224528590131No Hit
TTCAGTAGCGAATGGGTGAGCAACACGTACCTAACCTACCTTTAAGACTG996400.3010670658011019No Hit
TTACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAATA943570.2851042264933217No Hit
GAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGC936340.28291964712184237No Hit
TTATGATAGTAGAAGAAGTTGGAAAGCTTCAACATAGAAGGTGATATTCC923150.2789342250043027No Hit
TTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGC906680.27395773506678345No Hit
CTAATACCGGATATAGTTATTTATCGCATGATGAGTAATAGAAAGGAGCT899270.27171876782713456No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG893880.27009015333027797No Hit
TCGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGT888190.268370892386472No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG881990.26649753248285213No Hit
TTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT880130.26593552451176616No Hit
TTTTTTGAGAGTTTGATCCTAGCTCAGGATGAACGCTCGCTGTGTGCCTA839100.25353811211732696No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA810150.24479072998671486No Hit
TCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGC786050.23750879874845054No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT778210.2351399049348409No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT770970.23295230401512998No Hit
CCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGC755070.22814804232681452No Hit
AGTAGCGAATGGGTGAGCAACACGTACCTAACCTACCTTTAAGACTGG700820.21175614317014071No Hit
CTCAACATTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTGGTTAC695270.21007918390157776No Hit
ACGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTG680390.20558312013289012No Hit
TACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAATA676300.20434730690614733No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCG673320.20344688553311716No Hit
TCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG665800.20117468126292012No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA649170.1961498465536946No Hit
CAGTAGCGAATGGGTGAGCAACACGTACCTAACCTACCTTTAAGACTGG645050.19490496868225687No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA638830.19302556568217372No Hit
TGAAATTCCTTGTAGACTACGAGGTTGATAGGTTGGAAGTGTA638200.1928352081435801No Hit
TCGCTCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTG610740.1845380366994831No Hit
TTTTTTTGAGAGTTTGATCCTAGCTCAGGATGAACGCTCGCTGTGTGCCT601220.1816615227829571No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGG600020.18129893699515973No Hit
TGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCG592440.17900860343557284No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGT584700.17666992510427967No Hit
ATACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAAT573850.17339154527294492No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA573360.1732434894095943No Hit
AAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCC566380.17113444874390615No Hit
TTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTGGTTACCCGCATC557640.16849361558944845No Hit
GAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCC538700.16277080323871296No Hit
TAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGC533540.16121168435118416No Hit
GCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACC531990.1607433443752792No Hit
TTTAGTATATTGGCATATACCCGAAACCAGGTGATCTATCCATGAGC523460.15816596373368605No Hit
GGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCC512780.15493895022228926No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC510030.1541080244585869No Hit
TCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTG507590.15337076669006555No Hit
AAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGC504920.15256401331221633No Hit
AAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCG502500.15183279864015828No Hit
TTCAACATTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTGGTTAC500480.15122244589736597No Hit
ACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGAA496890.15013771008220544No Hit
GGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGAC496580.15004404208702446No Hit
TTACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAA493050.1489774355612538No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG486160.14689558882965043No Hit
AGGATGAACGCTCGCTGTGTGCCTAATACATGCATGTTGAACGGG477250.14420338935525479No Hit
ACTGTCTCAACATTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTG468530.1415685992972604No Hit
TTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGG464840.1404536479997834No Hit
AGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG460910.13926617954474696No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTC456060.13780072865239915No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA450550.13613585557676278No Hit
TCGCGAGGGAAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGT449860.13592736874877928No Hit
TCCTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATCTGCCGGGA437000.1320416577228839No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT434270.13121677505564483No Hit
ACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCAGGATGAAGTTCT433730.131053611451136No Hit
TTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCC429110.12965765616811603No Hit
TAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCC425720.1286333513175884No Hit
TTGAAATTCCTTGTAGACTACGAGGTTGATAGGTTGGAAGTGTA423310.127905158193762No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC415870.12565712630941814No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCTCCGCCGAGGGCGCACCACC394420.11917590535253973No Hit
TCTCAACATTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTGGTTA392300.11853533712743103No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC391160.11819088062902348No Hit
ATCCTAGCTCAGGATGAACGCTCGCTGTGTGCCTAATACATGCATGTTGA385780.11656528768039852No Hit
TTGGGAAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCAGGGC373780.11293942980242458No Hit
ACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAATA373080.11272792142620944No Hit
TGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCC368050.11120808266569203No Hit
AGGATGAACGCTCGCTGTGTGCCTAATACATGCATGTTGAACGG366980.11088477700490604No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG363950.1099692478907176No Hit
TCGAACCTGGAGATAGCTGGTTCTCTTCGAAATAGCTTTAGGGCTAGCGT360620.10896307232957986No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCG359550.10863976666879384No Hit
TTTATGATAGTAGAAGAAGTTGGAAAGCTTCAACATAGAAGGTGATATTC358770.10840408590672554No Hit
TACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTTGGTGGAA356220.10763359110765607No Hit
ACATTGGACTCGGTGAAATTATGGTACCAGTGAAAACGCTGGTTACCCGC346990.1048447020898478No Hit
TTTAGTATATTGGCATATACCCGAAACCAGGTGATCTATCCATGAGCAGG344950.10422830625059223No Hit
TCGGTCCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCTGCTCT341590.10321306604475954No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG335350.10132761994821307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)