FastQCFastQC Report
Tue 25 Jul 2023
SRR10513626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10513626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12292279
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG10522868.560544387253168TruSeq Adapter, Index 11 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC3739463.042120993186048Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG2427241.9746053599987439Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG1208180.9828771377545206TruSeq Adapter, Index 11 (100% over 45bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC1182670.9621242732938293TruSeq Adapter, Index 11 (100% over 42bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG946210.7697596190258943TruSeq Adapter, Index 11 (100% over 45bp)
GCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG940720.7652934008412924TruSeq Adapter, Index 11 (100% over 45bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT797730.6489683483428906Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG740470.6023862621406495TruSeq Adapter, Index 11 (100% over 45bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT703640.5724243649204512Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG690240.5615232130673246Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC682150.5549418460156982TruSeq Adapter, Index 11 (100% over 44bp)
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG678080.5516308245200097TruSeq Adapter, Index 11 (100% over 45bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA616960.5019085557690319Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC614870.5002083014874622No Hit
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT575430.4681231202122893TruSeq Adapter, Index 11 (100% over 46bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG534970.4352081497662069TruSeq Adapter, Index 11 (100% over 45bp)
GAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG529650.4308802297767566TruSeq Adapter, Index 11 (100% over 45bp)
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG524640.4268045006137593TruSeq Adapter, Index 11 (100% over 45bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG483960.39371055603277477TruSeq Adapter, Index 11 (100% over 45bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT463330.37692766329172966Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG437950.3562805562743898TruSeq Adapter, Index 11 (100% over 45bp)
CGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC428750.3487961833603029TruSeq Adapter, Index 11 (100% over 44bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT418090.34012407300550207TruSeq Adapter, Index 11 (100% over 43bp)
GCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGA404990.3294669767908782Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG392730.31949323636406235TruSeq Adapter, Index 11 (100% over 45bp)
GGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC388060.31569410359136824TruSeq Adapter, Index 11 (100% over 44bp)
ACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG370770.30162836362565476TruSeq Adapter, Index 11 (100% over 35bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC364890.29684487311099916No Hit
CCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC361290.29391620544896513TruSeq Adapter, Index 11 (100% over 44bp)
GGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG346220.2816564772081727TruSeq Adapter, Index 11 (100% over 45bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA338810.2756283029371526Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG327780.2666551906281984TruSeq Adapter, Index 11 (100% over 45bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGA316300.25731599486148987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT316050.25711261516273753TruSeq Adapter, Index 11 (100% over 46bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG315770.2568848299001349TruSeq Adapter, Index 11 (100% over 50bp)
ACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC313750.2552415219342158TruSeq Adapter, Index 11 (100% over 44bp)
GGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC305080.24818831398148383TruSeq Adapter, Index 11 (100% over 44bp)
GCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC300630.24456815534369178TruSeq Adapter, Index 11 (100% over 44bp)
GGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT295860.24068767069149669TruSeq Adapter, Index 11 (100% over 46bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG295420.24032972242169248TruSeq Adapter, Index 11 (100% over 45bp)
AGGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT293520.23878403671117454TruSeq Adapter, Index 11 (100% over 41bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT291800.2373847843837583TruSeq Adapter, Index 11 (100% over 46bp)
AGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG290480.23631093957434585TruSeq Adapter, Index 11 (100% over 45bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC279110.22706123087508834Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG274730.22349801855294696TruSeq Adapter, Index 11 (100% over 45bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC274550.22335158516984524TruSeq Adapter, Index 11 (100% over 50bp)
GGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG270480.22004056367415678TruSeq Adapter, Index 11 (100% over 45bp)
CGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC268630.21853555390338925TruSeq Adapter, Index 11 (100% over 44bp)
CGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT259780.21133591256755563TruSeq Adapter, Index 11 (100% over 43bp)
CGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG258400.21021325663044257TruSeq Adapter, Index 11 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC256570.20872451723557528TruSeq Adapter, Index 11 (100% over 50bp)
GGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC249480.20295666897895825TruSeq Adapter, Index 11 (100% over 44bp)
GGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC240820.1959115962141764TruSeq Adapter, Index 11 (100% over 44bp)
GGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG234790.19100607788026938TruSeq Adapter, Index 11 (100% over 45bp)
GGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC229860.18699543022087278TruSeq Adapter, Index 11 (100% over 44bp)
AGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC228210.1856531242091072TruSeq Adapter, Index 11 (100% over 44bp)
CGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC223990.1822200748941673TruSeq Adapter, Index 11 (100% over 44bp)
GAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG221100.17986900557659TruSeq Adapter, Index 11 (100% over 45bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT213050.17332017927676388TruSeq Adapter, Index 11 (100% over 48bp)
GAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC207780.16903293522706409TruSeq Adapter, Index 11 (100% over 44bp)
CCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG200670.16324881659454685TruSeq Adapter, Index 11 (100% over 45bp)
CGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC196640.15997033585065878TruSeq Adapter, Index 11 (100% over 44bp)
CCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAA191750.15599222894306256Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG190250.15477195075054836TruSeq Adapter, Index 11 (100% over 45bp)
ACGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC188950.15371437631703608TruSeq Adapter, Index 11 (100% over 42bp)
GGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC185890.15122500880430717TruSeq Adapter, Index 11 (100% over 44bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA183950.14964678234198883Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC182630.14857293753257633TruSeq Adapter, Index 11 (98% over 50bp)
CGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG181350.14753163347496426TruSeq Adapter, Index 11 (100% over 45bp)
AAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG179730.14621373302704893TruSeq Adapter, Index 11 (100% over 45bp)
TTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTCC179690.14618119227524853Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG178300.14505040115018542TruSeq Adapter, Index 11 (100% over 45bp)
GGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC176200.14334201168066557TruSeq Adapter, Index 11 (100% over 44bp)
GAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG171800.13976252898262395TruSeq Adapter, Index 11 (100% over 45bp)
GAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC171660.13964863635132263TruSeq Adapter, Index 11 (100% over 44bp)
AGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC170300.1385422507901098TruSeq Adapter, Index 11 (100% over 44bp)
CCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAACTCCA167930.1366142112459374Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC167490.13625626297613325TruSeq Adapter, Index 11 (100% over 44bp)
ACGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT165340.1345071975668629TruSeq Adapter, Index 11 (100% over 43bp)
CGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC165190.1343851697476115TruSeq Adapter, Index 11 (100% over 44bp)
GGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT163790.13324624343459826TruSeq Adapter, Index 11 (100% over 46bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG163180.1327499969696425TruSeq Adapter, Index 11 (100% over 45bp)
GCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG156970.12769804525263379TruSeq Adapter, Index 11 (100% over 45bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG155000.12609541322646517TruSeq Adapter, Index 11 (100% over 45bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA154370.1255828963856092Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC151430.12319115112828143TruSeq Adapter, Index 11 (100% over 44bp)
GGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC150550.1224752545886731TruSeq Adapter, Index 11 (100% over 44bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC147870.12029502421804776TruSeq Adapter, Index 11 (100% over 44bp)
GACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT142280.11574745415394493TruSeq Adapter, Index 11 (100% over 46bp)
AGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC142070.11557661520699294TruSeq Adapter, Index 11 (100% over 44bp)
GAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT141570.11516985580948821TruSeq Adapter, Index 11 (100% over 43bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGT134990.10981690213832601TruSeq Adapter, Index 11 (100% over 50bp)
TGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG134620.10951590018417252TruSeq Adapter, Index 11 (100% over 45bp)
GACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG134210.10918235747821865TruSeq Adapter, Index 11 (100% over 45bp)
GTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG134000.10901151853126667TruSeq Adapter, Index 11 (100% over 45bp)
CCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG133130.10830375717960844TruSeq Adapter, Index 11 (100% over 45bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT131510.10698585673169311TruSeq Adapter, Index 11 (100% over 46bp)
GCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCG130330.10602590455358198TruSeq Adapter, Index 11 (100% over 45bp)
AGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC129540.1053832247055245TruSeq Adapter, Index 11 (100% over 44bp)
GGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC128770.10475681523336722TruSeq Adapter, Index 11 (100% over 44bp)
GGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC127240.10351213147700275TruSeq Adapter, Index 11 (100% over 44bp)
GCGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC125890.10241388110374TruSeq Adapter, Index 11 (100% over 44bp)
CCCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT125170.1018281475713332TruSeq Adapter, Index 11 (100% over 43bp)
GCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC124280.10110411584377478TruSeq Adapter, Index 11 (100% over 44bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA123120.1001604340415638Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)