FastQCFastQC Report
Tue 18 Jul 2023
SRR10513628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10513628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14600572
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG13213759.050159130751863TruSeq Adapter, Index 10 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC6678564.574176956902784Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG3264332.2357548731652432Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT2378191.62883344570336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC1537691.0531710675444772TruSeq Adapter, Index 10 (100% over 42bp)
GGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG1258550.8619867769564096TruSeq Adapter, Index 10 (100% over 45bp)
GCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG992430.6797199452185846TruSeq Adapter, Index 10 (100% over 45bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG932670.6387900419243849TruSeq Adapter, Index 10 (100% over 45bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA868390.5947643695055235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC808740.5539098057254196TruSeq Adapter, Index 10 (100% over 44bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG789740.5408966169270628Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC782910.5362187180063904Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG778980.5335270426391514TruSeq Adapter, Index 10 (100% over 45bp)
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG763530.5229452654320667TruSeq Adapter, Index 10 (100% over 45bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT715740.49021367108083164Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG633940.4341884687805382TruSeq Adapter, Index 10 (100% over 45bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT630540.4318597928903059TruSeq Adapter, Index 10 (100% over 46bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC561110.3843068614024163No Hit
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG553500.37909473683633765TruSeq Adapter, Index 10 (100% over 45bp)
GAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG538100.36854720486293274TruSeq Adapter, Index 10 (100% over 45bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT521780.35736956058981795TruSeq Adapter, Index 10 (100% over 43bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG485120.3322609552557256TruSeq Adapter, Index 10 (100% over 45bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA481280.32963092130911037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT460850.315638318827509Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGA454030.3109672689535725Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG452980.3102481190462949TruSeq Adapter, Index 10 (100% over 45bp)
GGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC451410.30917281870874647TruSeq Adapter, Index 10 (100% over 44bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC440290.30155667873833986No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA435830.298502003894094Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC420360.2879065285935373TruSeq Adapter, Index 10 (100% over 44bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGA416190.2850504761046348Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG403410.27629739437605594TruSeq Adapter, Index 10 (100% over 45bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG392320.26870180154585727TruSeq Adapter, Index 10 (100% over 50bp)
GGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG383770.26284586658659675TruSeq Adapter, Index 10 (100% over 45bp)
TTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTCC375060.25688034687956063Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT370860.25400374725045016TruSeq Adapter, Index 10 (100% over 41bp)
GGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC368920.2526750321836706TruSeq Adapter, Index 10 (100% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC363010.2486272455627081TruSeq Adapter, Index 10 (100% over 50bp)
GGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG362420.24822315180528542TruSeq Adapter, Index 10 (100% over 45bp)
ACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC358350.24543558978374272TruSeq Adapter, Index 10 (100% over 44bp)
CCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC357760.24503149602632004TruSeq Adapter, Index 10 (100% over 44bp)
GCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC322370.22079271962769678TruSeq Adapter, Index 10 (100% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC316490.2167654801469422TruSeq Adapter, Index 10 (100% over 50bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT311530.2133683529658975TruSeq Adapter, Index 10 (100% over 46bp)
GGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT310160.21243003356306864TruSeq Adapter, Index 10 (100% over 46bp)
CGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT298520.20445774316239115TruSeq Adapter, Index 10 (100% over 43bp)
AGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG294670.20182086016903994TruSeq Adapter, Index 10 (100% over 45bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG294660.201814011122304TruSeq Adapter, Index 10 (100% over 45bp)
GGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC284830.1950813981808384TruSeq Adapter, Index 10 (100% over 44bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT273570.18736937155612807TruSeq Adapter, Index 10 (100% over 46bp)
GGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC271770.18613654314365216TruSeq Adapter, Index 10 (100% over 44bp)
CGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC271620.18603380744261253TruSeq Adapter, Index 10 (100% over 44bp)
GGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC265520.18185588893366642TruSeq Adapter, Index 10 (100% over 44bp)
CCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAACTCCA263590.18053402291362283Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAAC263100.18019841962355995Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG261990.1794381754358665TruSeq Adapter, Index 10 (100% over 45bp)
TCCCATGTCCCTTGGGAAGGTCTGAGATCGGAAGAGCACACGTCTGAACT261880.17936283592177074Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG259160.17749989520958495TruSeq Adapter, Index 10 (100% over 45bp)
CGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG254610.17438357894471532TruSeq Adapter, Index 10 (100% over 45bp)
GAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC242870.1663427980766781TruSeq Adapter, Index 10 (100% over 44bp)
AGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC240810.16493189444906678TruSeq Adapter, Index 10 (100% over 44bp)
GGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG235870.16154846536149406TruSeq Adapter, Index 10 (100% over 45bp)
CGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC226780.1553226818784908TruSeq Adapter, Index 10 (100% over 44bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT226620.15521309713071516TruSeq Adapter, Index 10 (100% over 48bp)
CCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA224810.15397341967150327Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC220180.15080231103274583TruSeq Adapter, Index 10 (98% over 50bp)
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC218420.14959687880721384Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC214410.146850411066087TruSeq Adapter, Index 10 (100% over 42bp)
GGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG214070.14661754347706377TruSeq Adapter, Index 10 (100% over 45bp)
GAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG213370.14613811020554537TruSeq Adapter, Index 10 (100% over 45bp)
CGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC211120.1445970746899505TruSeq Adapter, Index 10 (100% over 44bp)
CTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTCC206730.14159034317285651Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC202590.13875483782416195TruSeq Adapter, Index 10 (100% over 44bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA200630.13741242466391043Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG198040.13563852155929235TruSeq Adapter, Index 10 (100% over 45bp)
GGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC190150.13023462368460634TruSeq Adapter, Index 10 (100% over 44bp)
CCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAA189850.130029152282527Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC188430.12905658764601824TruSeq Adapter, Index 10 (100% over 44bp)
GGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC187970.1287415314961633TruSeq Adapter, Index 10 (100% over 44bp)
GAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG186300.12759774069125512TruSeq Adapter, Index 10 (100% over 45bp)
GAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC185630.12713885455994464TruSeq Adapter, Index 10 (100% over 44bp)
GGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT185330.12693338315786531TruSeq Adapter, Index 10 (100% over 46bp)
TTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCAG184360.12626902562447553Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT182840.125227970520607TruSeq Adapter, Index 10 (100% over 43bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG178000.12191303190039404TruSeq Adapter, Index 10 (100% over 45bp)
AAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG174760.11969394075793743TruSeq Adapter, Index 10 (100% over 45bp)
CGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG173590.1188926022898281TruSeq Adapter, Index 10 (100% over 45bp)
AGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG173570.11887890419635615TruSeq Adapter, Index 10 (100% over 45bp)
GGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC173120.11857069709323717TruSeq Adapter, Index 10 (100% over 44bp)
AGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC171850.11770086815776806TruSeq Adapter, Index 10 (100% over 44bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG169810.1163036626236287Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC162700.11143399039434893TruSeq Adapter, Index 10 (100% over 44bp)
CGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC159510.1092491444855722TruSeq Adapter, Index 10 (100% over 44bp)
GAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT158940.10885874882162151TruSeq Adapter, Index 10 (100% over 43bp)
GCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG158830.10878340930752575TruSeq Adapter, Index 10 (100% over 45bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA155230.10631775248257397Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC154850.10605748870660683TruSeq Adapter, Index 10 (100% over 44bp)
AGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAAC154820.10603694156639891Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTC154460.10579037588390372TruSeq Adapter, Index 10 (100% over 44bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA153680.1052561502384975Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG152550.10448220795733207Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA151890.1040301708727576Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT151230.10357813378818309TruSeq Adapter, Index 10 (100% over 46bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG148920.10199600399217236TruSeq Adapter, Index 10 (100% over 45bp)
GACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCG148830.10193436257154857TruSeq Adapter, Index 10 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)