FastQCFastQC Report
Tue 18 Jul 2023
SRR10513630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10513630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12092292
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC9625657.960153459741131Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG6351465.252486459969706TruSeq Adapter, Index 9 (100% over 49bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG5589024.621969102300871Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA2827402.3381836958617934Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG2353601.946363848970898Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA1608451.3301448559131719TruSeq Adapter, Index 9 (100% over 35bp)
GAGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA1322401.0935892054211063TruSeq Adapter, Index 9 (100% over 38bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT1105280.9140368095643077Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT1084790.8970921310864806Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG989770.8185131487066306TruSeq Adapter, Index 9 (100% over 45bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC961610.7952255866795145No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA882600.7298864433640868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT779600.6447082157791095Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG734160.6071305588717176TruSeq Adapter, Index 9 (100% over 45bp)
GCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG696360.5758709763211144TruSeq Adapter, Index 9 (100% over 45bp)
GCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGA666240.5509625470506336Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG587680.4859955416227131TruSeq Adapter, Index 9 (100% over 45bp)
GGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC559790.4629312623281013TruSeq Adapter, Index 9 (100% over 44bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGA555240.45916853479886194Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT551480.45605911600546856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT494780.40916974217956364TruSeq Adapter, Index 9 (100% over 46bp)
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG418340.3459559196883436TruSeq Adapter, Index 9 (100% over 45bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA402850.33314610662726307Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG378900.31334010128104745TruSeq Adapter, Index 9 (100% over 45bp)
AGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAAC358520.29648638984238884Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC330420.2732484462002737TruSeq Adapter, Index 9 (100% over 44bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG323540.26755887138683054TruSeq Adapter, Index 9 (100% over 45bp)
GGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG291930.24141825222215937TruSeq Adapter, Index 9 (100% over 45bp)
GGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTCC288930.23893733297211148Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGGCGCACCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT283390.2343559020903564TruSeq Adapter, Index 9 (100% over 36bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC281900.23312371219616596Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCAG275360.2277153082310616Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAA270670.2238368044701534Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG258470.21374773285329202TruSeq Adapter, Index 9 (100% over 45bp)
CCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC253700.20980307124571587TruSeq Adapter, Index 9 (100% over 44bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT251990.20838894727318855TruSeq Adapter, Index 9 (100% over 46bp)
TTGGTGACTCTAGATAACCTCGGAGATCGGAAGAGCACACGTCTGAACTC245040.20264148434391097Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC237640.1965218835271262TruSeq Adapter, Index 9 (100% over 44bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG234840.19420635889374818Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG222900.1843323002785576TruSeq Adapter, Index 9 (100% over 45bp)
GCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC220840.18262873572685806TruSeq Adapter, Index 9 (100% over 44bp)
CGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC218940.18105748686849443TruSeq Adapter, Index 9 (100% over 44bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT215890.17853521896427907TruSeq Adapter, Index 9 (100% over 46bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT214890.1777082458809298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG208400.17234119056999284TruSeq Adapter, Index 9 (100% over 45bp)
AGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG207860.17189462510498424TruSeq Adapter, Index 9 (100% over 45bp)
CGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT191440.15831572707638883TruSeq Adapter, Index 9 (100% over 43bp)
GGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC191300.1581999508447199TruSeq Adapter, Index 9 (100% over 44bp)
GGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG190610.1576293394172089TruSeq Adapter, Index 9 (100% over 45bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCT187280.15487551904965577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC186480.15421394058297633TruSeq Adapter, Index 9 (100% over 44bp)
CGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG184620.15267577064794663TruSeq Adapter, Index 9 (100% over 45bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA183370.15164205429376004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC176970.14634942656032454No Hit
TTGGTGACTCTAGATAACCTCGAGATCGGAAGAGCACACGTCTGAACTCC175460.1451006972044671Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG174360.14419102681278287TruSeq Adapter, Index 9 (100% over 45bp)
AACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAACTCCA174260.14410832950444796Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA172450.14261150822358573Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG169830.14044483874521058TruSeq Adapter, Index 9 (100% over 45bp)
GGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC167420.13845183361433877TruSeq Adapter, Index 9 (100% over 44bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC166960.13807142599599811TruSeq Adapter, Index 9 (100% over 42bp)
GGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGT166550.1377323670318249TruSeq Adapter, Index 9 (100% over 46bp)
ACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA164910.13637613117513206Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC164390.13594610517179043TruSeq Adapter, Index 9 (100% over 44bp)
GAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACT162070.13402752761842007Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG160670.13286976530173106Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC158420.13100907586419513TruSeq Adapter, Index 9 (100% over 44bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG156360.1293055113124956TruSeq Adapter, Index 9 (100% over 50bp)
TTGGTGACTCTAGATAACCTCAGATCGGAAGAGCACACGTCTGAACTCCA153590.12701479587161807Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT152860.12641110552077306TruSeq Adapter, Index 9 (100% over 48bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG152690.1262705200966037TruSeq Adapter, Index 9 (100% over 45bp)
GGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC150380.12436021227406682TruSeq Adapter, Index 9 (100% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC150130.1241534690032295TruSeq Adapter, Index 9 (100% over 50bp)
GAGGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT147880.1222927795656936TruSeq Adapter, Index 9 (100% over 41bp)
GAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC143160.11838946661228492TruSeq Adapter, Index 9 (100% over 44bp)
GAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG141340.11688437560058919TruSeq Adapter, Index 9 (100% over 45bp)
CGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC139500.1153627451272265TruSeq Adapter, Index 9 (100% over 44bp)
CCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAACTCCA138900.11486656127721692Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC138380.1144365352738753TruSeq Adapter, Index 9 (100% over 44bp)
TCCCATGTCCCTTGGGAAGGTCTGAGATCGGAAGAGCACACGTCTGAACT137350.11358475299802552Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTC134320.11107902455547716Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT132490.10956566381294795No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA130890.10824250687958908Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC130110.10759746787457664TruSeq Adapter, Index 9 (100% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC129640.10720879052540247TruSeq Adapter, Index 9 (100% over 50bp)
CCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG127830.10571196924454025TruSeq Adapter, Index 9 (100% over 45bp)
ACGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC127340.1053067524336991TruSeq Adapter, Index 9 (100% over 42bp)
GAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC125550.10382647061450385TruSeq Adapter, Index 9 (100% over 44bp)
CGGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTC125420.10371896411366846Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTCGCCGAATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA123680.10228003094864067TruSeq Adapter, Index 9 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)