FastQCFastQC Report
Tue 18 Jul 2023
SRR10513632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10513632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13451867
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG11666348.672654881288969TruSeq Adapter, Index 8 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC9720877.226409538542122Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG5690394.230186040346667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA3522302.6184469412312805Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC2747822.042705298825806TruSeq Adapter, Index 5 (100% over 35bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG2450101.8213828608326266Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT1212390.9012800974020929Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1166130.8668908189472881No Hit
GAGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG1097710.8160279907614312TruSeq Adapter, Index 8 (100% over 38bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT1016210.7554416052433465Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC966610.7185694000691503TruSeq Adapter, Index 8 (100% over 42bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA840160.6245675786119503Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT809630.6018718442577525Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGA742930.5522876489932587Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT650240.4833827155739794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG571560.42489269333394386TruSeq Adapter, Index 8 (100% over 45bp)
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG541450.40250918329775337TruSeq Adapter, Index 8 (100% over 45bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGA526800.3916185017291652Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA518380.385359147544352Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA488920.36345884180983945Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGGCGCACCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT461790.3432906376490341TruSeq Adapter, Index 8 (100% over 36bp)
AGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAAC459080.3412760474066537Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG447700.33281625517112234TruSeq Adapter, Index 8 (100% over 45bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG439780.3269285966029845TruSeq Adapter, Index 8 (100% over 45bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT424120.31528708988871207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG410150.3049019143588024TruSeq Adapter, Index 8 (100% over 45bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG388450.2887703245950915TruSeq Adapter, Index 8 (100% over 50bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG383340.2849715953926693TruSeq Adapter, Index 8 (100% over 45bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT358900.26680311364957743TruSeq Adapter, Index 8 (100% over 46bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT340450.25308754539425643TruSeq Adapter, Index 8 (100% over 43bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC332580.24723705638778617TruSeq Adapter, Index 8 (100% over 50bp)
GGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC319870.2377885538118984TruSeq Adapter, Index 8 (100% over 44bp)
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG315270.23436895413848502TruSeq Adapter, Index 8 (100% over 45bp)
CCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAA314370.23369990202846935Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC286130.2127065335986447TruSeq Adapter, Index 8 (100% over 50bp)
GGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG282150.20974783648990877TruSeq Adapter, Index 8 (100% over 45bp)
ACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA280190.20829078967254136Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTCC276080.20523545170346985Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC269950.2006784634430299TruSeq Adapter, Index 8 (100% over 44bp)
TTGGTGACTCTAGATAACCTCGGAGATCGGAAGAGCACACGTCTGAACTC267580.19891662621998865Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC264990.19699124292561024TruSeq Adapter, Index 8 (100% over 44bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC262350.1950286900695643Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG251280.18679934911637172TruSeq Adapter, Index 8 (100% over 45bp)
GGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG247920.18430155457231326TruSeq Adapter, Index 8 (100% over 45bp)
GGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG238940.1776259012968237TruSeq Adapter, Index 8 (100% over 45bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCT237540.17658515357013269Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG232980.17319528954605334TruSeq Adapter, Index 8 (100% over 45bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC224820.1671292170819114TruSeq Adapter, Index 8 (98% over 50bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA224580.16695080318590722Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT217530.16170989499078456TruSeq Adapter, Index 8 (100% over 41bp)
GACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG215460.16017107513774853TruSeq Adapter, Index 8 (100% over 45bp)
GGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT210780.15669200416566711TruSeq Adapter, Index 8 (100% over 46bp)
TTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCAG209570.15579250077331272Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCGCCGAATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC202660.15065566735085917TruSeq Adapter, Index 5 (100% over 35bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC200480.14903507446215458No Hit
ACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT200020.14869311449481323TruSeq Adapter, Index 8 (100% over 36bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA197980.14717659637877775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT189690.1410138830543002No Hit
GGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC188050.139794721431605TruSeq Adapter, Index 8 (100% over 44bp)
ACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA186690.138783709354248Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT185040.13755711381921928TruSeq Adapter, Index 8 (100% over 48bp)
TTGGTGACTCTAGATAACCTCGAGATCGGAAGAGCACACGTCTGAACTCC183360.13630821654719008Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTGAACT178980.13305216294511388Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT177580.13201141521842283TruSeq Adapter, Index 8 (100% over 46bp)
GAGGGCGCACCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC167990.12488229329058934TruSeq Adapter, Index 5 (100% over 35bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG163090.12123967624717075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC158600.11790184960942596TruSeq Adapter, Index 8 (100% over 44bp)
CACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA156760.11653400974006062Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT155010.11523307508169683TruSeq Adapter, Index 8 (100% over 46bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGT153580.1141700256180053TruSeq Adapter, Index 8 (100% over 50bp)
GGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG152460.11333742743665247TruSeq Adapter, Index 8 (100% over 45bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG150570.1119324180056196TruSeq Adapter, Index 8 (100% over 45bp)
CGGCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTC148000.11002190253590821Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT143530.10669894372283045TruSeq Adapter, Index 8 (100% over 46bp)
GGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG139980.10405990484443535TruSeq Adapter, Index 8 (100% over 45bp)
CGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTC139420.10364360575375894Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC138850.10321987275074902TruSeq Adapter, Index 8 (100% over 44bp)
TCCCATGTCCCTTGGGAAGGTCTGAGATCGGAAGAGCACACGTCTGAACT136030.10112350947269995Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)