FastQCFastQC Report
Tue 25 Jul 2023
SRR10513634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10513634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14541142
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG9879256.793998710692736TruSeq Adapter, Index 12 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC7166574.928478107152794Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG2906061.998508782872762Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT1998041.3740598915821054Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG1577191.0846397071151634TruSeq Adapter, Index 12 (100% over 45bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT1154760.7941329504931594Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG1147970.789463441041976TruSeq Adapter, Index 12 (100% over 45bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG1137740.7824282301898985TruSeq Adapter, Index 12 (100% over 45bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG931870.6408506292009253TruSeq Adapter, Index 12 (100% over 45bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG917280.6308170293639935Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC863510.5938391908971111TruSeq Adapter, Index 12 (100% over 44bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT857220.589513533393732TruSeq Adapter, Index 12 (100% over 46bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA836230.5750786286249044Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC784230.5393180260532495Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC737190.5069684348038139TruSeq Adapter, Index 12 (100% over 42bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA692940.4765375374231267Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT662750.4557757568146986Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG624880.42973241028799525TruSeq Adapter, Index 12 (100% over 45bp)
ACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT615910.4235637063443848TruSeq Adapter, Index 12 (100% over 35bp)
GAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG580010.3988751364920307TruSeq Adapter, Index 12 (100% over 45bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG538060.37002595807124367TruSeq Adapter, Index 12 (100% over 45bp)
CGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC524930.36099640592190074TruSeq Adapter, Index 12 (100% over 44bp)
GAGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT521140.3583900081575436TruSeq Adapter, Index 12 (100% over 38bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA499480.3434943417786581Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGA487050.33494618235624135Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT456260.313771779410448TruSeq Adapter, Index 12 (100% over 46bp)
GGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG448190.3082220089728853TruSeq Adapter, Index 12 (100% over 45bp)
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG432760.29761073786364234TruSeq Adapter, Index 12 (100% over 45bp)
CCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC423250.29107067381640317TruSeq Adapter, Index 12 (100% over 44bp)
TTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTCC421240.28968838898622956Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG415310.2856103048852697TruSeq Adapter, Index 12 (100% over 45bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC413080.2840767251980622No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGA404580.2782312420853878Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC395100.27171180915501686TruSeq Adapter, Index 12 (100% over 44bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT393020.27028138505215066TruSeq Adapter, Index 12 (100% over 46bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG379680.2611074150847299TruSeq Adapter, Index 12 (100% over 45bp)
GCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC359550.2472639356661258TruSeq Adapter, Index 12 (100% over 44bp)
CCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAACTCCA351160.2414940999819684Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG349860.24060008491767704TruSeq Adapter, Index 12 (100% over 45bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG345680.23772548263403243TruSeq Adapter, Index 12 (100% over 45bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG337420.2320450484563042TruSeq Adapter, Index 12 (100% over 45bp)
CGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC329240.22641963059022463TruSeq Adapter, Index 12 (100% over 44bp)
GGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT325760.2240264210335062TruSeq Adapter, Index 12 (100% over 46bp)
GGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC324360.22306363557965392TruSeq Adapter, Index 12 (100% over 44bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC319520.2197351487249076No Hit
CGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT314330.21616596550669817TruSeq Adapter, Index 12 (100% over 43bp)
GGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC306220.21058868691331123TruSeq Adapter, Index 12 (100% over 44bp)
GGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC299300.20582977595569865TruSeq Adapter, Index 12 (100% over 44bp)
GGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG295410.20315460780178063TruSeq Adapter, Index 12 (100% over 45bp)
CGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG289380.19900775331125986TruSeq Adapter, Index 12 (100% over 45bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT285690.19647012593646357TruSeq Adapter, Index 12 (100% over 48bp)
GGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG284730.1958099301966792TruSeq Adapter, Index 12 (100% over 45bp)
AGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC275000.18911857129240606TruSeq Adapter, Index 12 (100% over 44bp)
CGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC269180.18511613461996312TruSeq Adapter, Index 12 (100% over 44bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT261050.179525101948664TruSeq Adapter, Index 12 (100% over 43bp)
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC242590.1668300880357265Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAAC242350.1666650391007804Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG240600.16546155728346507TruSeq Adapter, Index 12 (100% over 45bp)
TTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCCAG236930.16293768398658098Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC236870.16289642175284444TruSeq Adapter, Index 12 (100% over 44bp)
GAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC235020.16162416954596825TruSeq Adapter, Index 12 (100% over 44bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG230610.15859139536633365TruSeq Adapter, Index 12 (100% over 50bp)
ACGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC230360.15841946939243148TruSeq Adapter, Index 12 (100% over 42bp)
GGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC228510.1571472171855553TruSeq Adapter, Index 12 (100% over 44bp)
CCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG222880.15327544425327805TruSeq Adapter, Index 12 (100% over 45bp)
CTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAAC222350.1529109611886054Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC220990.1519756838905775TruSeq Adapter, Index 12 (100% over 50bp)
CTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTCC219860.15119857848853963Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG219820.15117107033271526TruSeq Adapter, Index 12 (100% over 45bp)
GGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG219090.1506690464889209TruSeq Adapter, Index 12 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC214320.14738869890686715TruSeq Adapter, Index 12 (100% over 50bp)
GGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC213290.14668036389439013TruSeq Adapter, Index 12 (100% over 44bp)
CCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAA205660.1414331831708954Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA205630.14141255205402714Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG205190.14110996233995926TruSeq Adapter, Index 12 (100% over 45bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG202520.1392737929386839TruSeq Adapter, Index 12 (100% over 45bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA195940.13474870130557834TruSeq Adapter, Index 12 (100% over 47bp)
AAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG194910.13404036629310132TruSeq Adapter, Index 12 (100% over 45bp)
CGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC193170.13284376151474211TruSeq Adapter, Index 12 (100% over 44bp)
CCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA193150.13283000743682993Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG191560.131736558242812TruSeq Adapter, Index 12 (100% over 45bp)
GGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC190030.13068437128253063TruSeq Adapter, Index 12 (100% over 44bp)
TCCCATGTCCCTTGGGAAGGTCTGAGATCGGAAGAGCACACGTCTGAACT189200.13011357704917537Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT189080.13003105258170233TruSeq Adapter, Index 12 (100% over 46bp)
ACGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT185410.1275071792848182TruSeq Adapter, Index 12 (100% over 43bp)
GAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC185050.12725960588239907TruSeq Adapter, Index 12 (100% over 44bp)
GGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC178720.12290644022319568TruSeq Adapter, Index 12 (100% over 44bp)
TTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAACT177170.12184049918500212Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA177100.1217923599123095Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC177050.12175797471752907TruSeq Adapter, Index 12 (100% over 44bp)
AAGAGGCGGGCATGACACAGCAGATCGGAAGAGCACACGTCTGAACTCCA176140.1211321641725251Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT173930.11961233856322977TruSeq Adapter, Index 12 (100% over 41bp)
GCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG173460.11928911773229364TruSeq Adapter, Index 12 (100% over 45bp)
GGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG172050.11831945523948532TruSeq Adapter, Index 12 (100% over 45bp)
AGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC170550.11728789939607219TruSeq Adapter, Index 12 (100% over 44bp)
GAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT169970.11688903113661912TruSeq Adapter, Index 12 (100% over 43bp)
GGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC168290.11573368859199643TruSeq Adapter, Index 12 (100% over 44bp)
AGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC165140.11356742132082887TruSeq Adapter, Index 12 (100% over 44bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT164980.11345738869753147TruSeq Adapter, Index 12 (100% over 46bp)
GTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG160830.11060341753075516TruSeq Adapter, Index 12 (100% over 45bp)
TGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG158050.10869160070096281TruSeq Adapter, Index 12 (100% over 45bp)
GGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC156060.10732306994870142TruSeq Adapter, Index 12 (100% over 44bp)
GGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC154230.1060645718197374TruSeq Adapter, Index 12 (100% over 44bp)
CCCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT154070.10595453919644TruSeq Adapter, Index 12 (100% over 43bp)
ACGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT153390.10548690054742606TruSeq Adapter, Index 12 (100% over 43bp)
AGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC152650.10497799966467558TruSeq Adapter, Index 12 (100% over 44bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA150460.10347192813329241Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC149360.10271545384812279TruSeq Adapter, Index 12 (100% over 44bp)
ACCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT148960.1024403722898793TruSeq Adapter, Index 12 (100% over 43bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG148840.10235784782240624Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG147920.1017251602384462TruSeq Adapter, Index 12 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)