FastQCFastQC Report
Tue 18 Jul 2023
SRR10513636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10513636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13355848
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG156363511.707493226936993TruSeq Adapter, Index 7 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC3437122.5734943973606166Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG2050521.5352974966471615Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG1367441.0238511249903413TruSeq Adapter, Index 7 (100% over 45bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA1310790.981435248439485Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1215990.9104551055088377Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC1202610.9004370220445754TruSeq Adapter, Index 7 (100% over 42bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG1108980.8303328998652875TruSeq Adapter, Index 7 (100% over 45bp)
GCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG1055180.7900509200164602TruSeq Adapter, Index 7 (100% over 45bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG888970.6656035618255014TruSeq Adapter, Index 7 (100% over 45bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT768860.5756729187094671TruSeq Adapter, Index 7 (100% over 46bp)
ACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA761930.5704841804129547TruSeq Adapter, Index 7 (100% over 35bp)
GGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC733580.5492575237454035TruSeq Adapter, Index 7 (100% over 44bp)
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG706120.5286972418374334TruSeq Adapter, Index 7 (100% over 45bp)
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG653010.4889318896112025TruSeq Adapter, Index 7 (100% over 45bp)
GAGGGCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT643900.4821109075215591TruSeq Adapter, Index 7 (100% over 38bp)
GAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG574000.42977428314548055TruSeq Adapter, Index 7 (100% over 45bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG564300.4225115470017329TruSeq Adapter, Index 7 (100% over 45bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG541630.40553770902454117TruSeq Adapter, Index 7 (100% over 45bp)
GGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG518810.38845156069461106TruSeq Adapter, Index 7 (100% over 45bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC507620.380073208380329No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT498840.37349930906671Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG458910.3436022931677569TruSeq Adapter, Index 7 (100% over 45bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT431090.32277246641321466Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT421990.3159589716804205TruSeq Adapter, Index 7 (100% over 43bp)
CGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC417860.31286669330169076TruSeq Adapter, Index 7 (100% over 44bp)
GGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC410740.307535695225043TruSeq Adapter, Index 7 (100% over 44bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG405340.3034925225264618TruSeq Adapter, Index 7 (100% over 50bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT400420.2998087429566434TruSeq Adapter, Index 7 (100% over 46bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT368520.2759240746076176Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG366160.2741570583912006TruSeq Adapter, Index 7 (100% over 45bp)
CCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC365570.2737153043370964TruSeq Adapter, Index 7 (100% over 44bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA356880.26720879123512037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC356700.26707401881183435TruSeq Adapter, Index 7 (100% over 50bp)
GGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT354840.2656813704378786TruSeq Adapter, Index 7 (100% over 46bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC347520.26020062522424636TruSeq Adapter, Index 7 (100% over 50bp)
GGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG346750.25962409874685605TruSeq Adapter, Index 7 (100% over 45bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT345140.2584186342941309TruSeq Adapter, Index 7 (100% over 46bp)
GGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC331670.24833316461822566TruSeq Adapter, Index 7 (100% over 44bp)
ACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC330950.24779407492508151TruSeq Adapter, Index 7 (100% over 44bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG324260.2427850331929504TruSeq Adapter, Index 7 (100% over 45bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT323320.2420812216491233TruSeq Adapter, Index 7 (100% over 48bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG314710.2356346074019411TruSeq Adapter, Index 7 (100% over 45bp)
AGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG313560.2347735613642803TruSeq Adapter, Index 7 (100% over 45bp)
GGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG312120.23369538197799195TruSeq Adapter, Index 7 (100% over 45bp)
GCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGA306640.229592310424617Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG304710.22814725055271667TruSeq Adapter, Index 7 (100% over 45bp)
CGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC301630.22584114464315558TruSeq Adapter, Index 7 (100% over 44bp)
GCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC300870.22527210552261454TruSeq Adapter, Index 7 (100% over 44bp)
AGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACT274670.2056552305776466Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT264150.19777853117226252TruSeq Adapter, Index 7 (100% over 41bp)
CGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT255510.19130945485453263TruSeq Adapter, Index 7 (100% over 43bp)
GGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC249190.18657744532582282TruSeq Adapter, Index 7 (100% over 44bp)
GGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG244430.183013463465592TruSeq Adapter, Index 7 (100% over 45bp)
CGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC237920.17813919415674692TruSeq Adapter, Index 7 (100% over 44bp)
GGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC236190.1768438814218311TruSeq Adapter, Index 7 (100% over 44bp)
GAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG235920.17664172278690204TruSeq Adapter, Index 7 (100% over 45bp)
GGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC235270.17615504459170245TruSeq Adapter, Index 7 (100% over 44bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC228650.1711984143575159TruSeq Adapter, Index 7 (98% over 50bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA223620.16743227386235604Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG221680.1659797266336065TruSeq Adapter, Index 7 (100% over 45bp)
GGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG218150.1633366896658303TruSeq Adapter, Index 7 (100% over 45bp)
GAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC216890.16239328270282802TruSeq Adapter, Index 7 (100% over 44bp)
AGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC209840.15711469612412482TruSeq Adapter, Index 7 (100% over 44bp)
CGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG208970.15646329607824228TruSeq Adapter, Index 7 (100% over 45bp)
CGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC202960.15196339461185843TruSeq Adapter, Index 7 (100% over 44bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT202280.15145425434611115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG201080.15055577152420424TruSeq Adapter, Index 7 (100% over 45bp)
GGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC192030.14377971357565614TruSeq Adapter, Index 7 (100% over 44bp)
ACGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC191370.14328554802360732TruSeq Adapter, Index 7 (100% over 42bp)
GGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC190970.14298605374963835TruSeq Adapter, Index 7 (100% over 44bp)
GACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT187710.1405451754167912TruSeq Adapter, Index 7 (100% over 46bp)
GGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC187070.14006598457844086TruSeq Adapter, Index 7 (100% over 44bp)
GGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT185990.1392573500387246TruSeq Adapter, Index 7 (100% over 46bp)
GAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC185980.1392498626818754TruSeq Adapter, Index 7 (100% over 44bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG185500.13889046955311263TruSeq Adapter, Index 7 (100% over 45bp)
GCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG181980.1362549199421856TruSeq Adapter, Index 7 (100% over 45bp)
GAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG181780.13610517280520112TruSeq Adapter, Index 7 (100% over 45bp)
CGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC176940.13248129209017653TruSeq Adapter, Index 7 (100% over 44bp)
AAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG176150.1318897908990878TruSeq Adapter, Index 7 (100% over 45bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGA174700.13080412415595027Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT172910.1294638872799391TruSeq Adapter, Index 7 (100% over 50bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC170600.12773430784776824TruSeq Adapter, Index 7 (100% over 44bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA168950.12649889396764624TruSeq Adapter, Index 7 (100% over 47bp)
AGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAAC167070.12509127087999206Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT166490.12465700418273704TruSeq Adapter, Index 7 (100% over 46bp)
GCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG164010.12280013968412938TruSeq Adapter, Index 7 (100% over 45bp)
ACGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT163590.12248567069646195TruSeq Adapter, Index 7 (100% over 43bp)
GGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC162940.12199899250126237TruSeq Adapter, Index 7 (100% over 44bp)
AGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC162260.12148985223551512TruSeq Adapter, Index 7 (100% over 44bp)
GACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG161220.12071116712319577TruSeq Adapter, Index 7 (100% over 45bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG157120.11764135081501377TruSeq Adapter, Index 7 (100% over 45bp)
GCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC153870.11520795983901584TruSeq Adapter, Index 7 (100% over 44bp)
CCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAA152090.1138752103198539Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG151670.11356074133218647TruSeq Adapter, Index 7 (100% over 45bp)
TGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG150140.11241517573425513TruSeq Adapter, Index 7 (100% over 45bp)
GAGGGCGCACCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG149370.11183864925686485TruSeq Adapter, Index 7 (100% over 36bp)
CCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG146680.10982455026442349TruSeq Adapter, Index 7 (100% over 45bp)
CGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG144850.10845436396101542TruSeq Adapter, Index 7 (100% over 45bp)
GGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC143740.10762326735075153TruSeq Adapter, Index 7 (100% over 44bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT141480.10593112470282681TruSeq Adapter, Index 7 (100% over 46bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC141230.10574394078159621Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC140590.10526474994324583TruSeq Adapter, Index 7 (100% over 44bp)
GGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG140580.1052572625863966TruSeq Adapter, Index 7 (100% over 45bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA139330.10432134298024356Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC137540.1029811061042324TruSeq Adapter, Index 7 (100% over 44bp)
GAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT136180.10196282557273788TruSeq Adapter, Index 7 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)