FastQCFastQC Report
Thu 24 Aug 2023
SRR10530029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73209202
Total Bases7.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAATATTCCCAAACAATCTCCCATCCAAATACTAACCAAACCCAACCCT4844120.6616818470443101No Hit
CCCAATATTCCCAAACAATCTCCCATCCAAATACTAACCAAACCCAACCC3966070.5417447385917415No Hit
CCCCACTACCACAAATTATACAATCAAATTTCCCACATTTAAAAAAATCA3734820.5101571794212427No Hit
CTACAACACCCAATATTCCCAAACAATCTCCCATCCAAATACTAACCAAA3243940.443105499223991No Hit
CAAAAAAAACCCAAAACCACAAATACATTCAAAATATCAATAATCAATAT3114730.42545607859514706No Hit
CTCAAACAAACATAACCCCAAAAAAAACCCAAAACCACAAATACATTCAA2938620.4014003594793999No Hit
CCCAAACTAAAATACAATAACTATTCACAAACACAATCCCACTACTAATC2468020.33711882284961936No Hit
CCCACTACCACAAATTATACAATCAAATTTCCCACATTTAAAAAAATCAC2269100.3099473751947194No Hit
CCAAAAAAAACCCAAAACCACAAATACATTCAAAATATCAATAATCAATA2115680.28899099323606886No Hit
CCTACAACACCCAATATTCCCAAACAATCTCCCATCCAAATACTAACCAA1695850.23164437716449907No Hit
AAAAAAAACCCAAAACCACAAATACATTCAAAATATCAATAATCAATATA1585240.2165356207543418No Hit
CCCACTACTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTAA1555160.21242684765229378No Hit
CCCAAAAAAAACCCAAAACCACAAATACATTCAAAATATCAATAATCAAT1491520.2037339513685725No Hit
CAATATTCCCAAACAATCTCCCATCCAAATACTAACCAAACCCAACCCTA1411420.19279270384616404No Hit
CCACTACTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTAAA1353020.18481556457888995No Hit
CACTATATTACCCAAACTAAAATACAATAACTATTCACAAACACAATCCC1325520.18105920619104685No Hit
ACCCAATATTCCCAAACAATCTCCCATCCAAATACTAACCAAACCCAACC1305350.17830408805712702No Hit
CACCATATTAATACCAAACTTAATACAAACACCCAATCAACATAACACAC1280590.17492199955956356No Hit
CACTATATTACTCAAACTAAAATACAATAACTATTCACAAACACAATCCC1142530.15606371450408654No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1128750.15418143746465096TruSeq Adapter, Index 4 (100% over 50bp)
ACCCAATCAACATAACACACTACAACCCAAAACTCCTAAACTCAAACAAT1071210.14632176976877853No Hit
CCCCACTCCCAAAAAATCACCATATTAATACCAAACTTAATACAAACACC1050270.14346147359999908No Hit
CCCACATTTAAAAAAATCACAAAAATCAACACATCCAAAATACAATAAAT998000.13632166076608784No Hit
CCAAACTAAAATACAATAACTATTCACAAACACAATCCCACTACTAATCA975840.13329471887973865No Hit
CACTACTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTAAAC913300.12475207693153109No Hit
CCACTACCACAAATTATACAATCAAATTTCCCACATTTAAAAAAATCACA884360.12079902195901548No Hit
CACCATTCCTAAAAATACTACAATACCAAATCAATACATAAAATAAACAA879620.12015156236780179No Hit
CTCCATTTCCAACCTAAACCAATTCACCCCTCCTTAAACAACCTAATAAT748060.10218114384036038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)