FastQCFastQC Report
Thu 24 Aug 2023
SRR10530032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50526496
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTATATTACCCAAACTAAAATACAATAACTATTCACAAACACAATCCC7087681.4027649968048446No Hit
CACCATATTAATACCAAACTTAATACAAACACCCAATCAACATAACACAC6473381.2811852221060411No Hit
CCCAAACTAAAATACAATAACTATTCACAAACACAATCCCACTACTAATC6414361.269504222101608No Hit
CCCACTACTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTAA6084391.2041978925275167No Hit
CCCCACTACCACAAATTATACAATCAAATTTCCCACATTTAAAAAAATCA5520521.092599019730163No Hit
CACTATATTACTCAAACTAAAATACAATAACTATTCACAAACACAATCCC5346881.058232892302684No Hit
ACCCAATCAACATAACACACTACAACCCAAAACTCCTAAACTCAAACAAT5129611.015231691506967No Hit
CCACTACTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTAAA5049440.9993647689323242No Hit
CATATTAATACCAAACTTAATACAAACACCCAATCAACATAACACACTAC4817590.9534779534286327No Hit
CCCCACTCCCAAAAAATCACCATATTAATACCAAACTTAATACAAACACC4732430.9366234302097656No Hit
CACTACTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTAAAC3438830.680599343362342No Hit
CTACAAAATACCTCACCTCAACCATTAAACTCACTTCACTAACCATAAAT3367700.6665215810730275No Hit
CCCACTCCCAAAAAATCACCATATTAATACCAAACTTAATACAAACACCC3085310.6106320929122019No Hit
CTATATTACTCAAACTAAAATACAATAACTATTCACAAACACAATCCCAC3015420.5967997464142378No Hit
CTCCATTTCCAACCTAAACCAATTCACCCCTCCTTAAACAACCTAATAAT3001180.5939814231329241No Hit
CCCACTACCACAAATTATACAATCAAATTTCCCACATTTAAAAAAATCAC2994280.5926158030036359No Hit
CTCACTATATTACCCAAACTAAAATACAATAACTATTCACAAACACAATC2945360.5829337542029434No Hit
CCAAACTAAAATACAATAACTATTCACAAACACAATCCCACTACTAATCA2871480.5683117230215212No Hit
ATTAATACCAAACTTAATACAAACACCCAATCAACATAACACACTACAAC2852650.5645849654802898No Hit
ACCATATTAATACCAAACTTAATACAAACACCCAATCAACATAACACACT2831660.5604307094638029No Hit
ACTATATTACCCAAACTAAAATACAATAACTATTCACAAACACAATCCCA2810970.556335828235546No Hit
TCTCACTATATTACCCAAACTAAAATACAATAACTATTCACAAACACAAT2623530.5192384605494907No Hit
CTACTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTAAACCA2552220.5051250733872382No Hit
CTCACTATATTACTCAAACTAAAATACAATAACTATTCACAAACACAATC2438310.4825804662963369No Hit
TACTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTAAACCAA2335990.4623297051907182No Hit
AATCAACATAACACACTACAACCCAAAACTCCTAAACTCAAACAATCCTC2313570.45789242935033536No Hit
CACCCAATCAACATAACACACTACAACCCAAAACTCCTAAACTCAAACAA2238340.4430032116218786No Hit
ACCAAACTTAATACAAACACCCAATCAACATAACACACTACAACCCAAAA2182510.43195356353229003No Hit
CCTCACTATATTACCCAAACTAAAATACAATAACTATTCACAAACACAAT2154760.42646139562102225No Hit
CTATATTACCCAAACTAAAATACAATAACTATTCACAAACACAATCCCAC2146930.4249117136482213No Hit
GCCCAAACTAAAATACAATAACTATTCACAAACACAATCCCACTACTAAT2095040.41464185444405244No Hit
ACCTACTCCATTTCCAACCTAAACCAATTCACCCCTCCTTAAACAACCTA2010050.3978209769385156No Hit
GCCCAATCAACATAACACACTACAACCCAAAACTCCTAAACTCAAACAAT1987330.3933243263099028No Hit
CACAAACACAATCCCACTACTAATCAACACAAAAATTTTAACCTACTCCA1976620.3912046463700946No Hit
TCCATTTCCAACCTAAACCAATTCACCCCTCCTTAAACAACCTAATAATC1962660.38844173955779554No Hit
CCTTAAACAACCTAATAATCCCCCACTCCCAAAAAATCACCATATTAATA1957070.38733538933711137No Hit
CCTTAACCAAAAACCAATCCTCCTCTATCAAAAATAATCATCCTCTTCAA1926630.38131082749138195No Hit
TCTCACTATATTACTCAAACTAAAATACAATAACTATTCACAAACACAAT1910540.37812635968265046No Hit
CCAATCAACATAACACACTACAACCCAAAACTCCTAAACTCAAACAATCC1782610.35280697082180407No Hit
CCCAATCAACATAACACACTACAACCCAAAACTCCTAAACTCAAACAATC1738020.34398189813123No Hit
TCACTATATTACCCAAACTAAAATACAATAACTATTCACAAACACAATCC1721710.34075388881112995No Hit
CTCAAACTAAAATACAATAACTATTCACAAACACAATCCCACTACTAATC1684630.33341516498591156No Hit
CTACTCCATTTCCAACCTAAACCAATTCACCCCTCCTTAAACAACCTAAT1683630.33321724902514516No Hit
ACTATATTACTCAAACTAAAATACAATAACTATTCACAAACACAATCCCA1599380.31654282933057537No Hit
CAAAAAATCACCATATTAATACCAAACTTAATACAAACACCCAATCAACA1569400.31060930882679855No Hit
TCACTATATTACTCAAACTAAAATACAATAACTATTCACAAACACAATCC1510390.29893028798197285No Hit
CCTCACTATATTACTCAAACTAAAATACAATAACTATTCACAAACACAAT1509630.2987798718517904No Hit
CCATTTCCAACCTAAACCAATTCACCCCTCCTTAAACAACCTAATAATCC1490160.2949264480956685No Hit
CAATCAACATAACACACTACAACCCAAAACTCCTAAACTCAAACAATCCT1487090.2943188460961156No Hit
CAAACTTAATACAAACACCCAATCAACATAACACACTACAACCCAAAACT1484140.2937349940118547No Hit
CAAACTAAAATACAATAACTATTCACAAACACAATCCCACTACTAATCAA1456420.28824876357940993No Hit
CCATATTAATACCAAACTTAATACAAACACCCAATCAACATAACACACTA1380660.27325465039174696No Hit
GCCTACTCCATTTCCAACCTAAACCAATTCACCCCTCCTTAAACAACCTA1302370.25775980982334495No Hit
CCCCCACTCCCAAAAAATCACCATATTAATACCAAACTTAATACAAACAC1295970.2564931476744399No Hit
CTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTAAACCAATT1290780.2554659638380623No Hit
CCACTCCCAAAAAATCACCATATTAATACCAAACTTAATACAAACACCCA1221880.2418295541412569No Hit
CCCCTACAAAATACCTCACCTCAACCATTAAACTCACTTCACTAACCATA1076720.21310007327640532No Hit
CCACTACCACAAATTATACAATCAAATTTCCCACATTTAAAAAAATCACA1071090.21198580641729045No Hit
CCCCCACTACCACAAATTATACAATCAAATTTCCCACATTTAAAAAAATC1041930.20621457700134202No Hit
GCTCACTATATTACCCAAACTAAAATACAATAACTATTCACAAACACAAT1027490.20335667052787512No Hit
CCCTCCTTAAACAACCTAATAATCCCCCACTCCCAAAAAATCACCATATT1016900.20126074050335888No Hit
ATATTAATACCAAACTTAATACAAACACCCAATCAACATAACACACTACA1003510.19861064578869667No Hit
CTAAAATACAATAACTATTCACAAACACAATCCCACTACTAATCAACACA996640.19725096313823148No Hit
CCAACCTAAACCAATTCACCCCTCCTTAAACAACCTAATAATCCCCCACT928590.1837827820080775No Hit
ATATTACTCAAACTAAAATACAATAACTATTCACAAACACAATCCCACTA897150.17756030420158167No Hit
CCAAAAAATCACCATATTAATACCAAACTTAATACAAACACCCAATCAAC871670.17251740552125364No Hit
CCCCTCCTTAAACAACCTAATAATCCCCCACTCCCAAAAAATCACCATAT866300.17145459681193803No Hit
AAACTAAAATACAATAACTATTCACAAACACAATCCCACTACTAATCAAC842510.16674617610530523No Hit
CCTACATTCTCCTACAAATAAACCTTAAAAACTTATTTAAAAATTCTAAC834630.16518659833446594No Hit
CCTACTCCATTTCCAACCTAAACCAATTCACCCCTCCTTAAACAACCTAA831700.16460670456942036No Hit
TATTAATACCAAACTTAATACAAACACCCAATCAACATAACACACTACAA806860.15969047210398282No Hit
CCCCACTACTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTA785770.1555164244914193No Hit
CCAAACTTAATACAAACACCCAATCAACATAACACACTACAACCCAAAAC770700.15253383096266956No Hit
GCTCACTATATTACTCAAACTAAAATACAATAACTATTCACAAACACAAT753810.14919103038532497No Hit
CATTTCCAACCTAAACCAATTCACCCCTCCTTAAACAACCTAATAATCCC746470.14773832723329955No Hit
CTTAACCAAAAACCAATCCTCCTCTATCAAAAATAATCATCCTCTTCAAC746040.14765322337016998No Hit
GCCCACTACTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTA740000.1464578109671409No Hit
CACTACCACAAATTATACAATCAAATTTCCCACATTTAAAAAAATCACAA739540.14636676962518833No Hit
ATATTACCCAAACTAAAATACAATAACTATTCACAAACACAATCCCACTA728370.14415604834342757No Hit
TTCACAAACACAATCCCACTACTAATCAACACAAAAATTTTAACCTACTC721120.14272115762787113No Hit
CCTCCTTAAACAACCTAATAATCCCCCACTCCCAAAAAATCACCATATTA718940.14228970083340037No Hit
ATTCACAAACACAATCCCACTACTAATCAACACAAAAATTTTAACCTACT713700.1412526211989844No Hit
CCTCACCTCAACCATTAAACTCACTTCACTAACCATAAATCTATTCCAAA702440.13902408748075465No Hit
CTCACCTCAACCATTAAACTCACTTCACTAACCATAAATCTATTCCAAAC689440.13645117999079137No Hit
CCCCGCTCCCAAAAAATCACCATATTAATACCAAACTTAATACAAACACC681910.13496087280622032No Hit
ACTACTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTAAACC676590.13390795989494303No Hit
CTACATTCTCCTACAAATAAACCTTAAAAACTTATTTAAAAATTCTAACA647260.12810308476566432No Hit
CCCCGCTACCACAAATTATACAATCAAATTTCCCACATTTAAAAAAATCA646790.12801006426410413No Hit
CTTCACTAACCATAAATCTATTCCAAACTCCAACAAAAAAAACATCCACC642450.1271511089943779No Hit
ATCTCACTATATTACCCAAACTAAAATACAATAACTATTCACAAACACAA636510.12597548818742546No Hit
GCCATATTAATACCAAACTTAATACAAACACCCAATCAACATAACACACT632170.12511653291769925No Hit
CCTAAACCAATTCACCCCTCCTTAAACAACCTAATAATCCCCCACTCCCA630210.12472861763459711No Hit
CCCTTAACCAAAAACCAATCCTCCTCTATCAAAAATAATCATCCTCTTCA623110.12332341431315562No Hit
GCCCCCACTACCACAAATTATACAATCAAATTTCCCACATTTAAAAAAAT603400.11942249072644975No Hit
CACAAACACAATCCCCCACTACCACAAATTATACAATCAAATTTCCCACA602030.11915134586019976No Hit
TACAAAATACCTCACCTCAACCATTAAACTCACTTCACTAACCATAAATC600360.11882082620571988No Hit
CTAAAACTCAAAAAAAACCCTATTAAAACCACCTCTAACCCTAATCTCAA596450.11804697479912321No Hit
ACCACATACAACACCTCATTTAAATATATCTAAAATCTTAAAAACTTAAC592410.11724739431762693No Hit
ACCCAAACTAAAATACAATAACTATTCACAAACACAATCCCACTACTAAT591880.11714249885842075No Hit
GCCCAATCTAACCTCACACAAAACCCCATTCTCTAAAAACTCACCTCCCT579540.11470021590256328No Hit
CTCCTTAAACAACCTAATAATCCCCCACTCCCAAAAAATCACCATATTAA572630.1133326166136674No Hit
CCCTACAAAATACCTCACCTCAACCATTAAACTCACTTCACTAACCATAA552340.10931690176971703No Hit
CTCAACCATTAAACTCACTTCACTAACCATAAATCTATTCCAAACTCCAA543680.10760294954947994No Hit
TATTACTCAAACTAAAATACAATAACTATTCACAAACACAATCCCACTAC541850.10724076334127741No Hit
CCACACTAAAAATAAACCCCATATAATTAATACACAAACACACACTACCT540950.10706263897658766No Hit
TATATTACTCAAACTAAAATACAATAACTATTCACAAACACAATCCCACT536970.10627493345273735No Hit
TCCCACTACTAATCAACACAAAAATTTTAACCTACTCCATTTCCAACCTA532200.10533087431988158No Hit
AAACTTAATACAAACACCCAATCAACATAACACACTACAACCCAAAACTC531670.10522597886067539No Hit
CCTCTATCAAAAATAATCATCCTCTTCAACCAAACACACAACTTCAAAAA524630.1038326504968799No Hit
ACTCCATTTCCAACCTAAACCAATTCACCCCTCCTTAAACAACCTAATAA524210.10374952579335801No Hit
CACTCCCAAAAAATCACCATATTAATACCAAACTTAATACAAACACCCAA521480.10320921522046572No Hit
CTCCAACAAAAAAAACATCCACCAAACCCCTCCCCAAAAAACAAAATCCA514820.10189109492176145No Hit
GCTCCATTTCCAACCTAAACCAATTCACCCCTCCTTAAACAACCTAATAA514280.10178422030294759No Hit
CCCCATTCTCTAAAAACTCACCTCCCTAAAACTCAAAAAAAACCCTATTA511900.10131318031632354No Hit

[OK]Adapter Content

Adapter graph