FastQCFastQC Report
Tue 18 Jul 2023
SRR10530116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16232035
Total Bases811.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT267518616.4809033494568No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA183238511.288695471639878No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG170596810.50988369603688No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA8210575.0582505520718755No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG3932032.4223888132325984No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC3024131.8630627644654534No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCG2795981.7225073750765076No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTC2657881.6374287019464904No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG2180591.3433867041316754No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1907661.1752438927096942Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG1726431.0635943059511639No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA973420.5996906734121754No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG839940.5174582238148204Illumina Small RNA Adapter 2 (100% over 21bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGGAATTCTCGG739510.45558674559289697No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC721010.44418953014825313No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA688210.4239825751977494RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA630700.3885526368073997No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA517650.31890640945512994No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA497150.3062770626110651RNA PCR Primer, Index 1 (100% over 22bp)
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA454880.28023596548430313RNA PCR Primer, Index 5 (100% over 36bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC400220.24656181433812827RNA PCR Primer, Index 1 (100% over 24bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGCTGGAATTCTCGGGTGCCA396780.24444254833112422No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG396000.24396201708535006No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTTTGGTGACTCTAGATAAC391830.24139302311755734No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC352960.21744654936981098No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGTGGAATTCTCGGGTGCCAA351750.21670110987316132No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC351190.21635611308132344No Hit
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG346340.21336819443772762RNA PCR Primer, Index 5 (100% over 37bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA316440.19494783001638427No Hit
GGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGT302450.18632907087743467RNA PCR Primer, Index 5 (100% over 38bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTG296220.18249098156823837No Hit
CGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAT293580.1808645681210027RNA PCR Primer, Index 5 (100% over 41bp)
ACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA267010.16449570247969525RNA PCR Primer, Index 1 (100% over 32bp)
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA257570.1586800422744283RNA PCR Primer, Index 1 (100% over 34bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA244500.15062806357921235No Hit
GCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG243590.1500674437924758RNA PCR Primer, Index 5 (100% over 39bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA240740.14831165654830095RNA PCR Primer, Index 1 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAG231940.1428902783908487No Hit
CTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA227770.140321284423056RNA PCR Primer, Index 1 (100% over 34bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGG216300.1332550108473768No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA213930.13179493513906296No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGTGGAATTCTCGG208560.12848666233161768No Hit
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC204740.12613329135872367RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC199210.12272644803932471No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA197740.12182083146075029No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG191150.11776095849965824No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC186820.11509339401991187RNA PCR Primer, Index 5 (100% over 44bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG186170.11469295131510004RNA PCR Primer, Index 5 (100% over 39bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGA176910.10898818293578101RNA PCR Primer, Index 5 (100% over 40bp)
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC173730.10702909401070168RNA PCR Primer, Index 5 (100% over 35bp)
TCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG171410.10559982158737337RNA PCR Primer, Index 5 (100% over 37bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG169800.1046079558108395Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)