FastQCFastQC Report
Tue 18 Jul 2023
SRR10530117.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530117.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16562790
Total Bases828.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG210427012.704803961168379No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA16477379.94842656339904No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT13631588.230243817617684No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA6209833.749265673235005No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG4789162.8915176730490453No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC4388772.6497770001310164No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA4276822.582185730785695No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG3567142.153707195466464No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG3147551.90037427269198Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG1884981.1380812049177704Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1457220.8798155383241592No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA1245770.7521498491498111No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1212750.7322135944487613No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA1179950.7124101676106501RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA1023260.6178065410477341No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG833720.503369299496039No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA668120.4033861444841117No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC663000.4002948778557236No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG644230.3889622460950118No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC572690.3457690401194485No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG485790.2933020342587209No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTTTGGTGACTCTAGATAAC353250.21327928446837763No Hit
AACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC335300.20244173837861856RNA PCR Primer, Index 1 (100% over 31bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC327160.19752710744989221RNA PCR Primer, Index 1 (100% over 24bp)
GGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA308630.18633937881238608RNA PCR Primer, Index 6 (100% over 38bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGCTGGAATTCTCGGGTGCCA302870.18286170385544948No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA296420.1789674324192965RNA PCR Primer, Index 1 (100% over 22bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT283830.17136605608113126RNA PCR Primer, Index 1 (100% over 25bp)
AGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCC279700.16887251483596663RNA PCR Primer, Index 1 (100% over 27bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGTGGAATTCTCGGGTGCCAA270460.1632937445925475No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC268300.16198961648369628No Hit
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC262080.15823421054061543RNA PCR Primer, Index 6 (100% over 36bp)
CGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATAT259680.1567851793085585RNA PCR Primer, Index 6 (100% over 41bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAG259660.1567731040482914No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAA259250.15652556121281497No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA256560.15490143870688453No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG242140.14619517605427587Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCAC236590.14284429133014426No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTG235620.14225864120718792No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT209670.1265909910105725RNA PCR Primer, Index 6 (100% over 39bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG205050.12380160588886292Illumina Small RNA Adapter 2 (100% over 21bp)
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA202120.12203258025972677RNA PCR Primer, Index 1 (100% over 23bp)
GCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT200610.12112089810955765RNA PCR Primer, Index 6 (100% over 39bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC199550.12048090931539915RNA PCR Primer, Index 1 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT198370.11976846895963783No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAAC195460.11801151859076883No Hit
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA190860.1152342087293264RNA PCR Primer, Index 6 (100% over 37bp)
TGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAG187640.11329009182631669No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC186340.11250519990895255No Hit
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG184770.11155729197798198RNA PCR Primer, Index 6 (100% over 34bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA184220.1112252223206356No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC181840.10978826634884581RNA PCR Primer, Index 6 (100% over 44bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA179700.10849621350026173RNA PCR Primer, Index 1 (100% over 23bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA174000.10505476432412655RNA PCR Primer, Index 6 (100% over 40bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT169660.10243443284615696No Hit
TCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA168000.10143218624398426RNA PCR Primer, Index 6 (100% over 37bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT167890.10136577231251499No Hit
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC166110.10029107414873943RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)