FastQCFastQC Report
Tue 18 Jul 2023
SRR10530118.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530118.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22947376
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT319635013.929043564719557No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG307151513.385038010446163No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA18965808.264910114341614No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA10214414.451232245464579No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA7093253.091094162574405No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC5804132.5293218710496572No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG5673262.472291385298258No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG3610171.5732387005817134No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA3255871.4188419625843058No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2109590.9193164394918182Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG2086970.9094591032979108Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA1943330.8468637111275816No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1660250.7235032014117867No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG1122030.48895786603226443No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA990260.43153517857553736No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCAC935620.4077241772654093No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC931900.4061030768833874No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA923300.4023553716991433RNA PCR Primer, Index 1 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAG738810.32195837990365433No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC622320.27119440584404947No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG619000.2697476173310622No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA598030.26060931759692263No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT542000.23619258254189934No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT537900.23440588588429456No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT452880.1973558981209878No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTG421200.1835503980934465No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG347900.15160774809285382No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGCTGGAATTCTCGGGTGCCA314370.1369960556710275No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAA307520.13401096491380976No Hit
GGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT301040.13118711263544902RNA PCR Primer, Index 7 (100% over 38bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGTGGAATTCTCGGGTGCCAA292550.1274873432151894No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTTTGGTGACTCTAGATAAC290610.12664193065037155No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC289900.1263325270828351No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAAC283330.12346945463394159No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGTGGGGGGCCCAAGTC281090.12249330816734776No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA280510.12224055595724757RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGGGGGCCCAAGT271490.11830982331051706No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG270310.11779560329686498Illumina Small RNA Adapter 2 (100% over 21bp)
AACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC266100.11596097087527568RNA PCR Primer, Index 1 (100% over 31bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC249820.10886647780556696RNA PCR Primer, Index 1 (100% over 24bp)
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG240570.10483551583414157RNA PCR Primer, Index 7 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)