FastQCFastQC Report
Tue 18 Jul 2023
SRR10530119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13960537
Total Bases698 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG177466412.712003843405165No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA158324211.340838823033813No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT151016110.817356094539916No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA7773875.568460582855803No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG4015352.876214575413539No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC3326652.3828954430621114No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG2630411.8841753723370385No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA2421251.7343530553301783No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1740881.2470007421634284Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG1330000.9526854160409446Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA1297600.9294771397403981No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG1061140.7600997010358556No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA924170.6619874292801201No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG542700.3887386280341508No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC532310.381296220911846No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC529270.37911865424660957No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA523800.3752004668588322RNA PCR Primer, Index 1 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA427170.305983931706925No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG387760.27775435858950126No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGTGGAATTCTCGGGTGCCAA305930.21913913483413996No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGCTGGAATTCTCGGGTGCCA292530.20954065019132143No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTTTGGTGACTCTAGATAAC281600.2017114384640075No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG270770.1939538572191027No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA263970.1890829844152843RNA PCR Primer, Index 1 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAG246070.17626112806405655No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA235770.16888318837591992No Hit
GGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTG220680.1580741485803877RNA PCR Primer, Index 8 (100% over 38bp)
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT220360.1578449310366786RNA PCR Primer, Index 8 (100% over 36bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG219160.1569853652477695Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTG217980.15614012555534218No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC209750.15024493685307377No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC196280.14059631087256888RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA179360.12847643324895025No Hit
CGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAAT179030.12824005265700023RNA PCR Primer, Index 8 (100% over 41bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT167950.12030339520607265No Hit
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT159820.11447983698621336RNA PCR Primer, Index 1 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGTGGGGGGCCCAAGTC158840.11377785825860424No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGTGGAATTCTCGG158370.1134411949912815No Hit
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA154340.11055448655019502RNA PCR Primer, Index 1 (100% over 23bp)
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTT150520.10781820212216765RNA PCR Primer, Index 8 (100% over 37bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT150010.10745288666188127No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT149120.10681537536844034No Hit
GCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGA147930.10596297262777211RNA PCR Primer, Index 8 (100% over 39bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCAC146270.10477390661978117No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA143440.10274676396760382RNA PCR Primer, Index 1 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA143400.10271811177464019No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)