FastQCFastQC Report
Tue 18 Jul 2023
SRR10530120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19132408
Total Bases956.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG18389129.611503162591974No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC6433333.3625302157470194No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC4236622.2143684161450037RNA PCR Primer, Index 5 (100% over 50bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG3711221.9397558320939008Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT3349601.7507466911640186No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC2712461.4177305857161315No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2566261.3413157402873699No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2481771.29715506798726No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA2459051.2852799292174828No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG2444071.2774502822645222No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA2301091.202718445059294RNA PCR Primer, Index 1 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA2092501.093694008616166No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA1865700.9751516902629298No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT1407430.7356261689589727No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1244030.6502213417150627Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT876780.45826954976080375No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA867660.45350276870532974No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC776810.4060178938270604No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG758130.39625435543712006No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA740750.38717029241692946No Hit
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA716260.37437002179756984No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT694140.36280848704459995No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAA670770.3505936105899477No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA667270.3487642538252373No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC666110.3481579527260761No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT565200.2954149838326676No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC553120.2891010896276099RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG552810.2889390608855927No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG547080.285944142525081RNA PCR Primer, Index 5 (100% over 39bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC534750.27949957997968683No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGA517980.2707343477099171RNA PCR Primer, Index 5 (100% over 40bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAAC512030.2676244412099094No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAAC420270.2196639335728153No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTGGTGACTCTAGATA395720.20683230255177498No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGCC385760.2016264758727704No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG380060.19864723771309917No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCA373200.1950616984542667No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC365460.19101620663744992No Hit
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA361980.1891973033399664RNA PCR Primer, Index 1 (100% over 34bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGATA349940.1829043160693625No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG332830.17396137485673524RNA PCR Primer, Index 5 (100% over 45bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA316330.1653372643945289No Hit
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA315550.16492957917267917RNA PCR Primer, Index 1 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG285890.1494270872751616No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG273480.14294071086085974No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTGGAATTCTCGGGTGCCAA269170.1406879886734592No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCA263850.13790736639109935No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA256050.1338305141726018No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCC255540.13356395075831543No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGATA250260.1308042354104094No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA247790.12951323220788516RNA PCR Primer, Index 1 (100% over 22bp)
CAAAGCACCCAACTTACACTTAGGAGATTTGGAATTCTCGGGTGCCAAGG244030.12754798036922482Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC240940.1259329196826662RNA PCR Primer, Index 1 (100% over 26bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC238270.1245373818078728RNA PCR Primer, Index 5 (100% over 44bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAACC231400.1209466158154269No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG221840.11594985848096069Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC220180.11508222070112659RNA PCR Primer, Index 1 (100% over 24bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG218780.11435047799524241Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA213710.11170052405321902No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTTTGGTGACTCTAGATA213250.11146009430699994No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAG195250.10205197380277486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)