FastQCFastQC Report
Tue 18 Jul 2023
SRR10530121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20250485
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG19950549.8518825598498No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC6869803.3924125767851985No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC4262382.1048286004014227RNA PCR Primer, Index 5 (100% over 50bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG3991351.9709898306139335Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT3642701.7988211146547848No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC2935541.4496146635500335No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA2787391.3764559219198949No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2757341.361616771153876No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2685571.3261756446820903No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG2648861.3080476837962152No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA2465481.2174918279735027RNA PCR Primer, Index 1 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA2200621.0866998987925476No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA1935850.9559524129915901No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT1610510.7952945324519388No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1333310.6584089220579161Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT1011540.49951396225818795No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA923870.45622117198674506No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC833130.411412368642035No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG782140.3862327247964678No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT782120.3862228484898016No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA766850.37868228835013085No Hit
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA762190.376381108896898No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAA762110.37634160367023306No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA726270.3586432621243392No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC716160.3536507891045572No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC593020.2928423689605459RNA PCR Primer, Index 1 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT591080.2918843672139211No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC582920.287854834094097No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAAC581260.28703510064079946No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG574170.28353394992761904RNA PCR Primer, Index 5 (100% over 39bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG557680.2753909350813079No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGA532030.26272457178186104RNA PCR Primer, Index 5 (100% over 40bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAAC477320.2357079348963741No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTGGTGACTCTAGATA453610.223999573343552No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG414870.20486916733105406No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGCC410360.20264206017781794No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCA406100.20053840685790983No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGATA398350.19671133802474358No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC388900.19204478312494738No Hit
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA378080.186701701218514RNA PCR Primer, Index 1 (100% over 34bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG344510.17012432047923792RNA PCR Primer, Index 5 (100% over 45bp)
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA339600.1676996871926771RNA PCR Primer, Index 1 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA322080.1590480425530549No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG287730.1420854858537956No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCA283800.14014479159388035No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGATA283680.1400855337538829No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG282790.13964603810723547No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTGGAATTCTCGGGTGCCAA278410.13748312694732992No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCC272220.13442641003413006No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA269230.13294990218752786No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA265450.13108328022760937RNA PCR Primer, Index 1 (100% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAACC263520.13013021663431765No Hit
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC254590.12572044570784355RNA PCR Primer, Index 1 (100% over 26bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA253660.1252611974478636No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTGGAATTCTCGGGTGCCAAGG251730.12430813385457189Illumina Small RNA Adapter 2 (100% over 21bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC246360.12165634551468767RNA PCR Primer, Index 5 (100% over 44bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTTTGGTGACTCTAGATA240760.11889097964814177No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG237370.11721694566821485Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC230250.11370098049503505RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG224470.11084672786849303Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAG223610.11042204668184491No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA205700.10157781406223111No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGGAATTCTCGGGTGCCAAGG204690.1010790605755862Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)