FastQCFastQC Report
Tue 18 Jul 2023
SRR10530122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20917658
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG20208659.661048096302178No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC16247977.767585644626182RNA PCR Primer, Index 4 (100% over 50bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT9964384.763621242875278No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC4159001.9882723008474465No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA2311191.1048990283711493No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA2157601.031473026282388No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC1947340.9309550811089846No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG1810410.8654936417834156No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1453760.6949917624621266No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG1082360.5174384245119601Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1015930.4856805671074649No Hit
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC818000.3910571632828111RNA PCR Primer, Index 1 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG789960.3776522209130678No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT725610.34688873869149217No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT633110.30266772695107647No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA631090.3017020356676641No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA627530.3000001242968979No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA588750.28146076391534847RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG569840.27242055492063216No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG515990.24667675511283338No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC501010.23951534153584497No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG487890.23324312884358275Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA480700.22980584155262504RNA PCR Primer, Index 4 (100% over 40bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC459250.2195513474787665RNA PCR Primer, Index 1 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC430290.20570658531657796No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG428390.20479826183218025Illumina Small RNA Adapter 2 (100% over 21bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG390740.18679911489135162Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG382740.18297459495704538RNA PCR Primer, Index 4 (100% over 45bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA380570.18193719392486482No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCC375620.17957077221551285No Hit
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA364280.17414951520863378No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCA349430.16705025008057786No Hit
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA349270.16697375968189174RNA PCR Primer, Index 1 (100% over 23bp)
ATGTACACGGAGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA332710.15905700341787785RNA PCR Primer, Index 4 (100% over 36bp)
TTCCCGTGGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAAT327850.15673360755778684No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC322260.15406122425369034No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT315100.15063827891248627No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC306670.1466081910317111No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAATTGGAATTCTCGGGTGCC293700.14040768808821713No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG266780.1275381785092767No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA264080.12624740303144835RNA PCR Primer, Index 4 (100% over 39bp)
TCCCGTGGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATT263920.12617091263276223No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA257800.12324515488301797No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTG239160.11433402343608448No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCAC233180.11147519478519059No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGGAATTCTCGG232240.11102581369290959No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA229550.10973981886499913No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA227130.10858290158487151No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAA226510.10828650128996277No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG224590.10736861650572928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)