FastQCFastQC Report
Tue 18 Jul 2023
SRR10530123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22015882
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG21894939.945061478799714No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC16663507.5688541571943375RNA PCR Primer, Index 4 (100% over 50bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT10880944.942313916835128No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC4455862.0239298157575516No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA2436631.106760110723704No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA2333071.0597213411663453No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC2104000.9556737268123077No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG1842480.8368867529359033No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1451620.6593512810433849No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG1153240.5238218482457346Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1088540.49443397271115463No Hit
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC853000.3874475708036589RNA PCR Primer, Index 1 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG851340.38669356966938684No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT830820.3773730255276623No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT720370.3272046970455238No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA659710.2996518604160397No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA655150.2975806283845453No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG611150.27759505615082786No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA596900.271122456052408RNA PCR Primer, Index 1 (100% over 22bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG557390.25317632062163126No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC537170.24399204174513656No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG512850.2329454709105Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA508450.23094691368712822RNA PCR Primer, Index 4 (100% over 40bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC495300.2249739528945513RNA PCR Primer, Index 1 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC458210.20812702393662902No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG429170.1949365462623755Illumina Small RNA Adapter 2 (100% over 21bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG415150.1885684162006319Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA408070.18535255594120645No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCC402480.1828134798324228No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG401590.1824092262122408RNA PCR Primer, Index 4 (100% over 45bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA382310.1736519118334664No Hit
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA377470.1714534988877575RNA PCR Primer, Index 1 (100% over 23bp)
CGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCA375950.17076308821059272No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT358110.1626598471049218No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC356890.1621057016929869No Hit
TTCCCGTGGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAAT356870.1620966173419716No Hit
ATGTACACGGAGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA349750.15886258838051548RNA PCR Primer, Index 4 (100% over 36bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC332450.1510046247522584No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAATTGGAATTCTCGGGTGCC311820.1416341166799495No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA293680.13339461030904873No Hit
TCCCGTGGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATT286060.12993347257220947No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA275260.12502792302393337RNA PCR Primer, Index 4 (100% over 39bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG271910.12350629422886623No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCAC270780.12299302839650031No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTG261720.11887781738655757No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGGAATTCTCGG251970.11444919626658609No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA245540.11152857741515874No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAA238490.10832634368225628No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG233570.10609159333248606No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA230050.10449274755378866No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)