FastQCFastQC Report
Tue 18 Jul 2023
SRR10530124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25131472
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG279699711.12945950798266No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC17708967.04652715925275RNA PCR Primer, Index 7 (100% over 50bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA7464032.969993162358337No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC7286562.899376526770895No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT5776952.29869145746815No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA3988001.5868549203962266No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG3954511.573528999813461No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG3370411.3411112568336625No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA3214371.2790217779523618No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2908511.1573178045440395No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG2866501.140601712466345No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG2860241.1381108118139678Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG2783861.1077186405953459No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC1835510.7303631080582944No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1614670.6424892262578172No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1502390.5978121774960098Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA1321120.5256834935892334RNA PCR Primer, Index 7 (100% over 40bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA1194770.47540788697136405No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA1178010.4687389580681944RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG1135830.45195522172358227Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC1052930.41896869391494457RNA PCR Primer, Index 7 (100% over 39bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA913630.3635401857877644No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT850470.33840835109061657No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT841570.3348669747637544No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC653800.2601518924160113RNA PCR Primer, Index 1 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT651700.2593162867658528No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAA646190.2571238167028179No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC569320.22653667083249243No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCAC542120.21571358812567765No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAAC508030.20214892307143809No Hit
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC474540.18882300248867237RNA PCR Primer, Index 2 (100% over 34bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC451860.17979846146696063No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAG432710.17217853375242007No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG402820.1602850799984975Illumina Small RNA Adapter 2 (100% over 21bp)
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA381810.151925044422388RNA PCR Primer, Index 1 (100% over 23bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA359880.14319893399001857No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC356640.14190971384405976RNA PCR Primer, Index 7 (100% over 44bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTTTGGTGACTCTAGATAACCT331850.13204558809766495No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC331100.1317471575083226No Hit
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT318140.12659027692448735RNA PCR Primer, Index 1 (100% over 25bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG313600.12478377709033517RNA PCR Primer, Index 7 (100% over 45bp)
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCC303880.12091611665245872No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG300410.11953537779243492No Hit
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC290840.11572740347242692RNA PCR Primer, Index 7 (100% over 44bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC281820.11213827825126996No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC280890.1117682243204855No Hit
GGAATAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA263000.10464965999604002RNA PCR Primer, Index 7 (100% over 40bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC258050.10268001810638072RNA PCR Primer, Index 1 (100% over 26bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG256560.10208713600222065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)