FastQCFastQC Report
Tue 18 Jul 2023
SRR10530125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26365764
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG302913311.48888763473723No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC17962936.8129753418106915RNA PCR Primer, Index 7 (100% over 50bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA8073793.062224936853717No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC7773832.948456187349625No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT6219592.3589644510206496No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG4239841.6080853943773448No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA4041941.5330259346931878No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA3627801.3759510249731433No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG3626361.3754048621538144No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA3109151.1792375900808336No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG3072751.1654318077033534Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG2897791.0990730251548941No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG2760801.0471154941688776No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC1970280.7472872775467458No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1667540.6324641303775609No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1599430.6066313875827759Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA1371370.5201328510715639RNA PCR Primer, Index 7 (100% over 40bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA1246040.47259772180316867No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA1222210.46355948570274697RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG1139920.4323485562565151Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC1092290.41428346244774095RNA PCR Primer, Index 7 (100% over 39bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA973570.3692553722319596No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT963900.36558773718827187No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT958390.3634979058448676No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT739060.2803104814258369No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAA731610.2774848473952813No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC676200.2564689572431886RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCAC621980.23590440997651346No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC609370.2311216925100293No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAAC571310.21668630577137837No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAG498650.18912784017940842No Hit
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC490510.18604050313125764RNA PCR Primer, Index 2 (100% over 34bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC482060.1828355893650569No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG434030.16461878366202473Illumina Small RNA Adapter 2 (100% over 21bp)
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA402700.15273594954426506RNA PCR Primer, Index 1 (100% over 23bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA386290.14651196908232966No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC369080.13998456483187818RNA PCR Primer, Index 7 (100% over 44bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTTTGGTGACTCTAGATAACCT352350.13363921485453636No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC351690.13338889022901063No Hit
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT330100.12520024073643382RNA PCR Primer, Index 1 (100% over 25bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG323750.1227918144150877RNA PCR Primer, Index 7 (100% over 45bp)
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCC311270.11805840331423735No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC308850.11714054635397632No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG308280.11692435690465863No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC306830.1163744012879733No Hit
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC301030.11417457882123197RNA PCR Primer, Index 7 (100% over 44bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC277240.10515151391023601RNA PCR Primer, Index 1 (100% over 26bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG270710.10267481723647377No Hit
GGAATAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA269080.10205659126737235RNA PCR Primer, Index 7 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)