FastQCFastQC Report
Tue 18 Jul 2023
SRR10530126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34668693
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG32803189.461902702821822No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA11835803.4139735236052884No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG8341652.40610455086957No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC8248312.379181124595611No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT8209152.367885630992781No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC7049162.033292688593712RNA PCR Primer, Index 6 (100% over 50bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG5202881.5007430479135744No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA4974961.4350007368319306No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCAC2696330.7777420394821345No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA2225010.6417922936985251No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG2096060.6045973524297555Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC2020630.5828399703444257No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA1496020.4315190076533892No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1390110.40096983177300627No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT1304550.37629050509634154No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT1295880.3737896897353471No Hit
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT1270250.36639685263012367RNA PCR Primer, Index 6 (100% over 43bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC1199220.3459086271293816RNA PCR Primer, Index 6 (100% over 44bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1197000.3452682799435214No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTTTGGTGACTCTAGATAACC1126600.32496177459011794No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC1115560.3217773453415161RNA PCR Primer, Index 1 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG1037130.29915462922124003Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT1036020.29883445562830996No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTTTGGTGACTCTAGATAACCT983320.28363342108108897No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA927000.2673882167983662RNA PCR Primer, Index 6 (100% over 40bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAA877950.2532400053269963No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAAC863780.24915274423526726No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC851010.24546930569317973No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT819160.23628234268883455RNA PCR Primer, Index 6 (100% over 39bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA774760.22347539897163127No Hit
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT763190.2201380940435222RNA PCR Primer, Index 1 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG749910.21630754871549385No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA706370.20374866742164177No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG697730.2012565054009968Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA670380.1933675434490709RNA PCR Primer, Index 1 (100% over 22bp)
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCC630330.18181533408253953No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAG624570.1801538927354429No Hit
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC618300.1783453446024054RNA PCR Primer, Index 1 (100% over 24bp)
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA591860.1707188673077465RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTTTGGTGACTCTAGATAACC584940.168722830133804No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCA556100.16040408561118816No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCACC547770.15800134144082098No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG514010.14826344910089342RNA PCR Primer, Index 6 (100% over 45bp)
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC496200.14312624938009633RNA PCR Primer, Index 1 (100% over 24bp)
AACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC480090.1384794056124354RNA PCR Primer, Index 1 (100% over 31bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC477830.13782752063944262RNA PCR Primer, Index 6 (100% over 44bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG475120.13704583556120792Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC458890.13236437843214915No Hit
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG453320.1307577415739324RNA PCR Primer, Index 6 (100% over 34bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG442320.12758485011246315No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCGCCGAGGGCGCACC435850.12571861304376258No Hit
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT412310.1189286253162183RNA PCR Primer, Index 6 (100% over 46bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG410220.11832577593853912RNA PCR Primer, Index 6 (100% over 45bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG386980.11162232161448947No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC385260.111126196767787No Hit
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG381550.11005606701123691RNA PCR Primer, Index 6 (100% over 45bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTTTGGTGACTCTAGATAAC366820.10580727689965122No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC349060.1006844994127699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)