FastQCFastQC Report
Tue 18 Jul 2023
SRR10530127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36067484
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG35578739.864489022855045No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA11535363.198271329378007No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT8910772.470582644467244No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC8755322.427482881811218No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG7957992.206416727044226No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC6978021.9347121634544842RNA PCR Primer, Index 6 (100% over 50bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA5680881.5750696666282573No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG5119981.4195556307725816No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCAC3131270.8681697897197376No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA2203330.6108909620643348No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC2153080.5969587454450662No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG2018570.5596647661921746Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT1499340.41570407295391054No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT1479280.4101422766278901No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1453460.40298347397894463No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA1438530.3988440114092794No Hit
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT1279340.3547073036755211RNA PCR Primer, Index 6 (100% over 43bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1267360.3513857523302707No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC1235940.342674304645148RNA PCR Primer, Index 6 (100% over 44bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTTTGGTGACTCTAGATAACC1202780.3334804279666417No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC1189170.3297069460126467RNA PCR Primer, Index 1 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT1174860.3257393834291297No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG1093310.30312899008979943Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTTTGGTGACTCTAGATAACCT1055270.2925820941654816No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAA1000760.2774687582865497No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAAC990060.2745020972352826No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA945860.26224729177116984RNA PCR Primer, Index 6 (100% over 40bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC928310.25738141313100743No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT847990.2351120471835518RNA PCR Primer, Index 6 (100% over 39bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA824270.22853548642317273No Hit
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT788250.21854865174404736RNA PCR Primer, Index 1 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG777650.2156097164969977No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG752900.20874757995317889Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA733040.2032412352355934No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAG716460.1986442968963402No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCC673280.18667229463524543No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTTTGGTGACTCTAGATAACC646380.17921405330075144No Hit
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC642050.17801352597813588RNA PCR Primer, Index 1 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCACC637410.1767270486624462No Hit
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA628840.17435094724101077RNA PCR Primer, Index 1 (100% over 23bp)
CGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCA587900.16300000299438686No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA587490.1628863271970953RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG525690.1457517801907114RNA PCR Primer, Index 6 (100% over 45bp)
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC524530.14543016086178898RNA PCR Primer, Index 1 (100% over 24bp)
AACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC507990.14084431284422277RNA PCR Primer, Index 1 (100% over 31bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCGCCGAGGGCGCACC502380.13928889522762386No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC485630.13464482302120104No Hit
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC481900.13361065052389015RNA PCR Primer, Index 6 (100% over 44bp)
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG471910.13084084268256693RNA PCR Primer, Index 6 (100% over 34bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG463760.1285811896388448Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG424840.11779030663741336No Hit
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT420750.11665632124491968RNA PCR Primer, Index 6 (100% over 46bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG418310.11597981162201389RNA PCR Primer, Index 6 (100% over 45bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC407320.11293274573851617No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTTTGGTGACTCTAGATAAC406770.1127802538153202No Hit
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG393780.10917867184747347RNA PCR Primer, Index 6 (100% over 45bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG387100.10732658812574783No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC374540.10384422711603614No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)