FastQCFastQC Report
Tue 18 Jul 2023
SRR10530128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17540631
Total Bases877 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA16237429.257032999554008RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC13630117.7705927454947314RNA PCR Primer, Index 8 (100% over 50bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC6704713.822388145557591RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG3049941.738785793966021Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT3010751.716443382224961RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2648181.509740442062774Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2123531.2106348967719576RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC2035381.160380148239821RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG1847501.0532688362237368No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA1830791.0437423830419783RNA PCR Primer, Index 1 (100% over 23bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1805041.0290621814004297RNA PCR Primer, Index 1 (100% over 25bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGA1297860.7399163690291416RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA1275430.7271289157157459RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA1227250.6996612607608016No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG1218380.6946044301370915No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC1203770.6862751972833816No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1193690.6805285397087482RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA939690.5357218905066756RNA PCR Primer, Index 1 (100% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA936440.5338690495227908No Hit
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA929080.5296730773254394No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT919550.5242399774557711RNA PCR Primer, Index 1 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA744750.42458563776867553No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA716610.40854288537282385No Hit
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC653260.3724267388100234RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA653020.372289913629675No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA581630.33159012352520273No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG552290.31486324522760895No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA518080.2953599559787786RNA PCR Primer, Index 1 (100% over 23bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGG508780.29005798024027757Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAA487580.2779717559761675RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG486070.2771108975498088No Hit
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA464030.26454578515447935RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC451170.2572142359074768RNA PCR Primer, Index 1 (100% over 24bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAA446490.25454614489068267RNA PCR Primer, Index 8 (100% over 40bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG436590.24890210620131054RNA PCR Primer, Index 8 (100% over 45bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC411200.23442714233028447RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA391910.22342981845978063No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG388060.22123491452502478No Hit
CCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAAC355650.20275781412880758RNA PCR Primer, Index 1 (100% over 24bp)
CCCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAA341900.19491887150467962RNA PCR Primer, Index 1 (100% over 23bp)
TTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACTC333730.19026111432365234RNA PCR Primer, Index 1 (100% over 26bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC333050.1898734429793318RNA PCR Primer, Index 1 (100% over 24bp)
TTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAACT325960.18583139910987237RNA PCR Primer, Index 1 (100% over 25bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG315720.17999352474834002Illumina Small RNA Adapter 2 (100% over 21bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT312030.17788983760048313RNA PCR Primer, Index 8 (100% over 46bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT260200.14834129969440665No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG248260.141534246972073Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT245490.13995505634888505RNA PCR Primer, Index 8 (100% over 43bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG240280.1369848097254882RNA PCR Primer, Index 8 (100% over 45bp)
GCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC228470.13025187064250995RNA PCR Primer, Index 1 (100% over 27bp)
GGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTC214450.12225899969049005RNA PCR Primer, Index 1 (100% over 26bp)
TAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGAA210580.12005269365737185RNA PCR Primer, Index 1 (100% over 23bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC193070.11007015654111874No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA183170.10442611785174662No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGTGGAATTCTCGGGTGCCAAGGA181910.10370778565491742RNA PCR Primer, Index 1 (100% over 22bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAAGTGGAATTCTCGGGTGCCAAG175820.10023584670357641No Hit
ATGTACACGGAGTCGACCCAACGCGACTCCCGGGGCTACGCCTGTCTGAG175440.10001920683469141No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)