FastQCFastQC Report
Tue 18 Jul 2023
SRR10530129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18312587
Total Bases915.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA17501579.557125926555326RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC13902707.591882020819887RNA PCR Primer, Index 8 (100% over 50bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC6965083.8034385857115653RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG3273631.7876392887580548Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT3121941.704805552596146RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2845511.553854733905155Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC2145471.171582147295737RNA PCR Primer, Index 1 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2111111.152819096504497RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG2001581.0930077765637374No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA1899091.0370408069597157RNA PCR Primer, Index 1 (100% over 23bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1897991.0364401272196004RNA PCR Primer, Index 1 (100% over 25bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGA1327970.7251678858918186RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA1322270.722055272693039RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA1322130.721978822544297No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC1293840.7065304317735118No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG1274980.6962315045929884No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1263100.6897441633997424RNA PCR Primer, Index 1 (100% over 24bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA989750.5404752479810745No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT980880.5356315849857806RNA PCR Primer, Index 1 (100% over 25bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA964040.5264357242371054No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA917170.5008413065832806RNA PCR Primer, Index 1 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA797410.4354436650594479No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA758010.4139284089134975No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA705030.3849974883395776No Hit
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC692620.37822072872609425RNA PCR Primer, Index 1 (100% over 26bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA605940.3308871652050035No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG595300.3250769539006149No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA561560.30665246805380364RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG527800.2882170607571721No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGG521570.28481502913815504Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAA513360.2803317739869304RNA PCR Primer, Index 1 (100% over 23bp)
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA486240.2655222880306316RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC484110.26435915362477186RNA PCR Primer, Index 1 (100% over 24bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAA467110.25507592127753437RNA PCR Primer, Index 8 (100% over 40bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG456740.24941314954571958RNA PCR Primer, Index 8 (100% over 45bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC441980.2413531195783534RNA PCR Primer, Index 1 (100% over 27bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG412420.2252112167439805No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA411070.22447401888111168No Hit
CCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAAC382890.20908569608433805RNA PCR Primer, Index 1 (100% over 24bp)
CCCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAA365130.19938744864393RNA PCR Primer, Index 1 (100% over 23bp)
TTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACTC348700.1904154776165705RNA PCR Primer, Index 1 (100% over 26bp)
TTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAACT347820.1899349338244782RNA PCR Primer, Index 1 (100% over 25bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC345770.18881548521789956RNA PCR Primer, Index 1 (100% over 24bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG330910.18070084800143202Illumina Small RNA Adapter 2 (100% over 21bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT318610.1739841563619602RNA PCR Primer, Index 8 (100% over 46bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT276640.151065493914104No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT254760.13911742781071837RNA PCR Primer, Index 8 (100% over 43bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG252040.1376321106351604Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG248870.13590106083864611RNA PCR Primer, Index 8 (100% over 45bp)
GCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC247890.13536590979745242RNA PCR Primer, Index 1 (100% over 27bp)
GGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTC230710.12598438440183246RNA PCR Primer, Index 1 (100% over 26bp)
TAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGAA224120.12238576668605042RNA PCR Primer, Index 1 (100% over 23bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC209450.11437488324287552No Hit
ATGTACACGGAGTCGACCCAACGCGACTCCCGGGGCTACGCCTGTCTGAG204640.11174827456109833No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGTGGAATTCTCGGGTGCCAAGGA194920.10644044994844257RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA193050.10541929439024644No Hit
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT187990.10265616758571576RNA PCR Primer, Index 1 (100% over 25bp)
TAGTTCAACTTTAAATTTGCCCACAGAACCCTGGAATTCTCGGGTGCCAA187010.10212101654452208No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG185530.10131282925782141No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAAAGTGGAATTCTCGGGTGCCAAG183230.10005686252848929No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)