FastQCFastQC Report
Tue 18 Jul 2023
SRR10530130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15629790
Total Bases781.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC8441395.400833920353376RNA PCR Primer, Index 9 (100% over 50bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA5208923.3326871314329876RNA PCR Primer, Index 1 (100% over 22bp)
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA2379901.522669210526821RNA PCR Primer, Index 1 (100% over 28bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA2021011.2930500025912057RNA PCR Primer, Index 1 (100% over 22bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC1982881.2686542813435113RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA1461350.93497737333643RNA PCR Primer, Index 1 (100% over 23bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1364900.8732682908727499RNA PCR Primer, Index 1 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG1338120.8561343434556702Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1330190.851060698832166RNA PCR Primer, Index 1 (100% over 24bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT1280850.8194927762944991RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1013020.6481341080078491Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA938170.6002447889574972RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT927670.5935268484093517RNA PCR Primer, Index 1 (100% over 25bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGA880350.5632513296723757RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC840880.5379982712499656RNA PCR Primer, Index 1 (100% over 24bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC811250.5190408828269605RNA PCR Primer, Index 1 (100% over 27bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG765090.48950753656958923No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG730170.46716558571804223No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA606460.388015449983653RNA PCR Primer, Index 9 (100% over 40bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC593020.3794164860820267RNA PCR Primer, Index 1 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC523490.3349309235760685No Hit
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA503690.3222628071138512No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG492030.31480269408610095No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA483760.3095115161496092No Hit
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC482740.3088589162106465RNA PCR Primer, Index 1 (100% over 24bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC472990.302620828558797RNA PCR Primer, Index 1 (100% over 24bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA443860.28398334206665604No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG439230.28102105018685475RNA PCR Primer, Index 9 (100% over 45bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG419110.2681481964888844Illumina Small RNA Adapter 2 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA402000.25720115241471575No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGTGGAATTCTCGGGTGCCAAGGA393760.2519291685940758RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA384410.24594700248691762No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG379280.2426648086762522No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG378720.24230651851368445RNA PCR Primer, Index 9 (100% over 45bp)
TTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACTC375330.24013758342242603RNA PCR Primer, Index 1 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA369010.2360940230163041No Hit
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG356800.22828201786460342RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGG325050.2079682452547347Illumina Small RNA Adapter 2 (100% over 21bp)
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA319390.20434695539735337RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC314040.20092400473710778RNA PCR Primer, Index 1 (100% over 24bp)
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA312870.20017543421888584RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAA312670.20004747344654025RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG291120.18625970022629865No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA263820.16879305480112017RNA PCR Primer, Index 1 (100% over 23bp)
CCCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAA254280.16268932596023364RNA PCR Primer, Index 1 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA251810.16110901042176512No Hit
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG250740.16042442028971599RNA PCR Primer, Index 6 (100% over 34bp)
TTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAACT250110.16002134385682726RNA PCR Primer, Index 1 (100% over 25bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC246990.15802515580823542RNA PCR Primer, Index 9 (100% over 44bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT229460.146809394112141RNA PCR Primer, Index 9 (100% over 43bp)
GCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC228870.14643190983372137RNA PCR Primer, Index 1 (100% over 27bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC224040.1433416571815744RNA PCR Primer, Index 9 (100% over 44bp)
CCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAAC223540.14302175525071034RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA223110.14274663959016723No Hit
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT221280.14157579852320473RNA PCR Primer, Index 9 (100% over 46bp)
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC220920.14134546913298257RNA PCR Primer, Index 9 (100% over 44bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG218700.1399251045599461Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA217520.1391701360031069RNA PCR Primer, Index 1 (100% over 23bp)
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC215310.13775616946868768RNA PCR Primer, Index 1 (100% over 24bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG200720.128421431126074RNA PCR Primer, Index 9 (100% over 39bp)
TTGATCATCGACACTTCGAACGTGGAATTCTCGGGTGCCAAGGAACTCCA197990.12667476658355614RNA PCR Primer, Index 1 (100% over 28bp)
CCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC190190.1216842964620766RNA PCR Primer, Index 1 (100% over 27bp)
CACTCGCCGAATCCCGGGGCCGAGGGATGGAATTCTCGGGTGCCAAGGAA189870.12147955922632357RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG189500.12124283179748416No Hit
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC187920.12023194169595369RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG177890.11381470896282037No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA177180.11336044822099338No Hit
CACTCGCCGAATCCCGGGGCCGAGGGTGGAATTCTCGGGTGCCAAGGAAC170990.10940006231689613RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC166240.10636099397368744RNA PCR Primer, Index 1 (100% over 24bp)
AAGACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA161920.10359704129102182RNA PCR Primer, Index 9 (100% over 40bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG161150.10310439231749115RNA PCR Primer, Index 9 (100% over 45bp)
TAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGAA157800.10096104938070184RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)