FastQCFastQC Report
Tue 18 Jul 2023
SRR10530131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16229543
Total Bases811.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC8537255.260314477123601RNA PCR Primer, Index 9 (100% over 50bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA5594783.4472812943654665RNA PCR Primer, Index 1 (100% over 22bp)
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA2454871.512593422994104RNA PCR Primer, Index 1 (100% over 28bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC2096681.2918909669853305RNA PCR Primer, Index 1 (100% over 26bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA1951231.2022704521008387RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA1506450.9282146761618612RNA PCR Primer, Index 1 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG1429940.8810722520036455Illumina Small RNA Adapter 2 (100% over 21bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1421990.8761737776596668RNA PCR Primer, Index 1 (100% over 25bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT1357050.8361603281127509RNA PCR Primer, Index 1 (100% over 25bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1272040.7837805414483945RNA PCR Primer, Index 1 (100% over 24bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1086990.6697600788882349Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT978580.6029621413246202RNA PCR Primer, Index 1 (100% over 25bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA955410.5886857072931752RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC896830.5525910372214424RNA PCR Primer, Index 1 (100% over 24bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGA886630.5463062022140734RNA PCR Primer, Index 1 (100% over 22bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC868040.534851782332996RNA PCR Primer, Index 1 (100% over 27bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG823610.5074757804332506No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG756470.4661067782376867No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA626040.38574099098169307RNA PCR Primer, Index 9 (100% over 40bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC624700.3849153361866074RNA PCR Primer, Index 1 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC558740.3442734031389547No Hit
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA533910.32897414301807515No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA524180.322978903349281No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC507670.312806096881471RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC505880.3117031699537072RNA PCR Primer, Index 1 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG504570.31089599996746675No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA450190.27738920313406235No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG448410.276292437809247RNA PCR Primer, Index 9 (100% over 45bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG424540.26158469157141395Illumina Small RNA Adapter 2 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA422330.2602229773198173No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGTGGAATTCTCGGGTGCCAAGGA418960.25814651712620623RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA413030.25449268657780444No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG410480.2529214778259622No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA391750.24138079550360725No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG389020.23969867789869376RNA PCR Primer, Index 9 (100% over 45bp)
TTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACTC387610.23882989188296921RNA PCR Primer, Index 1 (100% over 26bp)
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG378700.23333990365594398RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA338620.20864419903875298RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC336910.2075905649345764RNA PCR Primer, Index 1 (100% over 24bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAA329450.20299400913506932RNA PCR Primer, Index 1 (100% over 23bp)
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA328500.20240865685497123RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGG327650.20188492060435712Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG313630.193246353270699No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA284480.1752852806761102RNA PCR Primer, Index 1 (100% over 23bp)
CCCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAA266680.16431762742795653RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA264110.1627340954702175No Hit
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG263670.16246298494048786RNA PCR Primer, Index 6 (100% over 34bp)
TTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAACT261530.16114440190953006RNA PCR Primer, Index 1 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA259910.1601462222318891No Hit
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC256190.15785410593508395RNA PCR Primer, Index 9 (100% over 44bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT238630.14703433115769185RNA PCR Primer, Index 9 (100% over 43bp)
GCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC238180.14675705902501385RNA PCR Primer, Index 1 (100% over 27bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC236140.14550009202354003RNA PCR Primer, Index 9 (100% over 44bp)
CCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAAC235520.14511807264073917RNA PCR Primer, Index 1 (100% over 24bp)
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC233020.14357767190363893RNA PCR Primer, Index 1 (100% over 24bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA231130.14241312894639116RNA PCR Primer, Index 1 (100% over 23bp)
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC227420.1401271742525344RNA PCR Primer, Index 9 (100% over 44bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT224350.1382355621473753RNA PCR Primer, Index 9 (100% over 46bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG220760.13602354668889938Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG215070.13251759461125923RNA PCR Primer, Index 9 (100% over 39bp)
TTGATCATCGACACTTCGAACGTGGAATTCTCGGGTGCCAAGGAACTCCA212330.13082931540339737RNA PCR Primer, Index 1 (100% over 28bp)
CACTCGCCGAATCCCGGGGCCGAGGGATGGAATTCTCGGGTGCCAAGGAA207110.12761295866433206RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC200890.12378044163042667RNA PCR Primer, Index 1 (100% over 26bp)
CCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC195440.12042236802354817RNA PCR Primer, Index 1 (100% over 27bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG192120.11837671584467904No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA183390.11299763647072503No Hit
CACTCGCCGAATCCCGGGGCCGAGGGTGGAATTCTCGGGTGCCAAGGAAC179830.11080410582109429RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC176160.10854279753903114RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG175870.10836411105352751No Hit
AAGACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA171830.10587482346237354RNA PCR Primer, Index 9 (100% over 40bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG166600.10265230512035983RNA PCR Primer, Index 9 (100% over 45bp)
TAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGAA166430.10254755787023702RNA PCR Primer, Index 1 (100% over 23bp)
AAAGCTGAACAAAATGAGTGAAAACTTGGAATTCTCGGGTGCCAAGGAAC164070.10109341957441438RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)