FastQCFastQC Report
Tue 18 Jul 2023
SRR10530132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31752363
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA28481778.9699686287915RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGC12870014.0532447931513005RNA PCR Primer, Index 14 (100% over 50bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC10424223.2829745616097923RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG8503762.6781502844370983Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG5898631.8576979609360098No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG4787251.5076830659815774Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT4024851.2675749518232706RNA PCR Primer, Index 1 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA3221231.0144851266660058No Hit
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA3214851.0124758273896024No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC3187461.0038496977374567RNA PCR Primer, Index 1 (100% over 24bp)
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA3014810.9494757917702061RNA PCR Primer, Index 1 (100% over 28bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA2643700.8325994509448006RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2623940.8263762920573816No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT2084130.6563700471678281No Hit
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1882020.592718091563768RNA PCR Primer, Index 1 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1507330.4747142755958037No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1482320.46683769645742584No Hit
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC1087460.3424815973538725RNA PCR Primer, Index 1 (100% over 27bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC1033430.3254655409425749RNA PCR Primer, Index 1 (100% over 24bp)
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA909020.2862842050527074RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA878620.2767101144566784RNA PCR Primer, Index 1 (100% over 22bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC865960.2727230096229374RNA PCR Primer, Index 1 (100% over 26bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCA836460.2634323624985013RNA PCR Primer, Index 14 (100% over 40bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG806340.2539464543158568No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG783290.24668715207117028Illumina Small RNA Adapter 2 (100% over 21bp)
CACAAAGCACCCAACTTACACTTAGGAGATTGGAATTCTCGGGTGCCAAG711820.2241785910547823No Hit
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA699960.22044343597356833RNA PCR Primer, Index 1 (100% over 34bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA696040.21920888218618564RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC684010.21542018778255967RNA PCR Primer, Index 1 (100% over 24bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCC666080.2097733639540465RNA PCR Primer, Index 14 (100% over 39bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG653920.2059437277156349No Hit
GGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTC644070.2028415963876452RNA PCR Primer, Index 1 (100% over 26bp)
CCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAAC627320.1975663984441095RNA PCR Primer, Index 1 (100% over 24bp)
CACAAAGCACCCAACTTACACTTAGGAGATTTGGAATTCTCGGGTGCCAA623260.19628775345003455No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT616670.19421231736359276RNA PCR Primer, Index 1 (100% over 25bp)
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT592650.18664752604396717RNA PCR Primer, Index 1 (100% over 25bp)
ATGTACACGGAGTCGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT585920.18452799875083312RNA PCR Primer, Index 1 (100% over 30bp)
GAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGGAACT528650.16649154584180081RNA PCR Primer, Index 1 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA519650.16365711112587117No Hit
ATGTACACGGAGTCGAGCTCAACCCGTGGAATTCTCGGGTGCCAAGGAAC500180.15752528402374338RNA PCR Primer, Index 1 (100% over 24bp)
CCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC499200.15721664557689768RNA PCR Primer, Index 1 (100% over 27bp)
CACAAAGCACCCAACTTACACTTAGGAGATGGAATTCTCGGGTGCCAAGG485180.15280122616386063Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT478610.15073208882123199RNA PCR Primer, Index 1 (100% over 25bp)
ATGTACACGGAGTCGAGCTCAACCTGGAATTCTCGGGTGCCAAGGAACTC466940.1470567718062432RNA PCR Primer, Index 1 (100% over 26bp)
GCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC464930.14642374805301892RNA PCR Primer, Index 1 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA463560.14599228410181628No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA459040.1445687680000383No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC458250.1443199676194178No Hit
GGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCC438330.1380464187814935RNA PCR Primer, Index 1 (100% over 27bp)
CATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAAG430200.13548597942143709No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA420110.1323082631676893RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCG381000.11999106964102169RNA PCR Primer, Index 14 (100% over 45bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA366470.11541503226074859No Hit
AGCCCGTGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCC356900.11240108334614342RNA PCR Primer, Index 14 (100% over 39bp)
CCCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAA353920.11146257051798003RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA353730.11140273245175486RNA PCR Primer, Index 1 (100% over 22bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC346980.10927690641480761RNA PCR Primer, Index 1 (100% over 24bp)
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA344270.10842342662812214RNA PCR Primer, Index 1 (100% over 28bp)
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC340920.107368387039415RNA PCR Primer, Index 1 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA335630.10570236930082968No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC325270.102439620005604No Hit
ATGTACACGGAGTCGAGCTCAACCCTGGAATTCTCGGGTGCCAAGGAACT317530.1000020061499045RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)