FastQCFastQC Report
Tue 18 Jul 2023
SRR10530133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33261334
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA30685799.225664250267291RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGC13045573.9221427498969224RNA PCR Primer, Index 14 (100% over 50bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC11140263.3493124479012177RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG9143792.7490749469038134Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG6395861.9229114502743636No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG5155971.5501392698200258Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT4319651.2987001663853892RNA PCR Primer, Index 1 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA3453221.0382085096166016No Hit
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA3410211.0252775790652293No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC3217870.9674506741070578RNA PCR Primer, Index 1 (100% over 24bp)
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA3151050.9473612814206429RNA PCR Primer, Index 1 (100% over 28bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2834390.8521576434667354No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA2592170.7793343466019733RNA PCR Primer, Index 1 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT2214780.6658722707874555No Hit
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1991640.5987853644114214RNA PCR Primer, Index 1 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1612850.4849023794415461No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1599470.4808796905139162No Hit
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC1164290.35004308606503876RNA PCR Primer, Index 1 (100% over 27bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC1100420.33084060909884133RNA PCR Primer, Index 1 (100% over 24bp)
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA966820.29067384970187904RNA PCR Primer, Index 1 (100% over 22bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC916620.2755812499883498RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA902250.2712609181580029RNA PCR Primer, Index 1 (100% over 22bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCA865510.2602150593238383RNA PCR Primer, Index 14 (100% over 40bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG823330.24753366777171354No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG793070.23843601702806028Illumina Small RNA Adapter 2 (100% over 21bp)
CACAAAGCACCCAACTTACACTTAGGAGATTGGAATTCTCGGGTGCCAAG748200.22494587859885595No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA747560.22475346298497828RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC735030.22098632604452967RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA730740.21969654013275597RNA PCR Primer, Index 1 (100% over 34bp)
GGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTC695740.20917381124882123RNA PCR Primer, Index 1 (100% over 26bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCC695010.20895433718924203RNA PCR Primer, Index 14 (100% over 39bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG675730.2031578168211774No Hit
CCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAAC671360.201843978957669RNA PCR Primer, Index 1 (100% over 24bp)
CACAAAGCACCCAACTTACACTTAGGAGATTTGGAATTCTCGGGTGCCAA658770.19805880305341933No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT646180.19427362714916965RNA PCR Primer, Index 1 (100% over 25bp)
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT635090.19093942533994576RNA PCR Primer, Index 1 (100% over 25bp)
ATGTACACGGAGTCGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT623430.1874338533746121RNA PCR Primer, Index 1 (100% over 30bp)
GAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGGAACT571910.17194439645746018RNA PCR Primer, Index 1 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA541400.16277158336463596No Hit
ATGTACACGGAGTCGAGCTCAACCCGTGGAATTCTCGGGTGCCAAGGAAC533390.1603633816971983RNA PCR Primer, Index 1 (100% over 24bp)
CCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC523480.15738394617606138RNA PCR Primer, Index 1 (100% over 27bp)
CACAAAGCACCCAACTTACACTTAGGAGATGGAATTCTCGGGTGCCAAGG517050.15545077055538423Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT507330.1525284584196172RNA PCR Primer, Index 1 (100% over 25bp)
GCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC501440.15075763347314933RNA PCR Primer, Index 1 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA497170.1494738605493093No Hit
ATGTACACGGAGTCGAGCTCAACCTGGAATTCTCGGGTGCCAAGGAACTC494970.1488124318766048RNA PCR Primer, Index 1 (100% over 26bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC490640.14751061998896375No Hit
GGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCC469000.14100456704472525RNA PCR Primer, Index 1 (100% over 27bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA466860.14036117733582182No Hit
CATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAAG459320.1380942808848256No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA432850.13013609135460413No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA426400.1281969027459933RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCG388680.11685640750307849RNA PCR Primer, Index 14 (100% over 45bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA385100.11578008266295031RNA PCR Primer, Index 1 (100% over 22bp)
CCCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAA380820.11449330324514344RNA PCR Primer, Index 1 (100% over 23bp)
AGCCCGTGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCC372410.11196484181903227RNA PCR Primer, Index 14 (100% over 39bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC370320.11133648457996304RNA PCR Primer, Index 1 (100% over 24bp)
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC369500.11108995207468227RNA PCR Primer, Index 1 (100% over 24bp)
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA363910.10940932194721956RNA PCR Primer, Index 1 (100% over 28bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC350030.10523630832124772No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA340720.1024372624381211No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC339100.10195021041549325RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)