FastQCFastQC Report
Tue 18 Jul 2023
SRR10530134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23664473
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA306189012.938762676016491RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG20660648.730657133163286Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC16241056.863051630179975RNA PCR Primer, Index 1 (100% over 24bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT12803115.410266267074699RNA PCR Primer, Index 1 (100% over 25bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC7362393.111157387700964RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG7205803.0449864655764785No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA5121762.1643245552098285No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA4944612.089465503837757No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG4892252.0673395093142366Illumina Small RNA Adapter 2 (100% over 21bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC4195191.7727798121682237RNA PCR Primer, Index 1 (100% over 27bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC3607011.5242300134889968RNA PCR Primer, Index 1 (100% over 24bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC3547821.4992178359518085RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG3115521.3165389315874476No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA3090301.3058816057302438No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGC2526081.0674566891897403RNA PCR Primer, Index 15 (100% over 50bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT2245220.9487724488941714RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA1719400.7265743885359289RNA PCR Primer, Index 1 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT1662170.7023904567830435No Hit
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA1282780.5420699628510637RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG1250020.5282264261705722No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA1222170.5164577296946354RNA PCR Primer, Index 1 (100% over 23bp)
GGCCGAGGTGGGATCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1103670.4663826656946892RNA PCR Primer, Index 1 (100% over 32bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT1054200.4454779111286358RNA PCR Primer, Index 1 (100% over 25bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA905510.38264532660414624RNA PCR Primer, Index 1 (100% over 23bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCA894370.37793784801377156RNA PCR Primer, Index 15 (100% over 39bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA872080.3685186650892247RNA PCR Primer, Index 1 (100% over 23bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAA768790.3248709574052209RNA PCR Primer, Index 15 (100% over 40bp)
TGCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCA759040.32075085720269364No Hit
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA735130.3106471037829577No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA593270.2507007022721359RNA PCR Primer, Index 1 (100% over 22bp)
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA588470.24867234524935333No Hit
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA532420.2249870512645686RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC506100.21386489358964383RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGAACT489720.20694312524939812RNA PCR Primer, Index 1 (100% over 25bp)
TTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTC448170.18938515977093595RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGA440630.1861989489476482RNA PCR Primer, Index 1 (100% over 22bp)
GGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTC378290.15985566211425878RNA PCR Primer, Index 1 (100% over 26bp)
GGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAAC377070.1593401213709682RNA PCR Primer, Index 1 (100% over 24bp)
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT365930.1546326427805935RNA PCR Primer, Index 1 (100% over 25bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG365610.15449741897907465No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG358100.15132388538717934No Hit
CCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGAAC345360.14594028778921042RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG316390.13369830800795773Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA297560.12574123243733337RNA PCR Primer, Index 1 (100% over 28bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC254110.1073803756373531No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT237700.10044593006571496RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)