FastQCFastQC Report
Tue 18 Jul 2023
SRR10530135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24507582
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA327555913.365492360690661RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG22000598.977054529492138Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC15511006.329061757296171RNA PCR Primer, Index 1 (100% over 24bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT13316655.4336857875248565RNA PCR Primer, Index 1 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG7798823.1822070410699843No Hit
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC7760703.1666526709978977RNA PCR Primer, Index 1 (100% over 26bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA5484002.237674855071382No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA5316992.1695285973132723No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG5196692.1204417473743433Illumina Small RNA Adapter 2 (100% over 21bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC4445291.8138427528264518RNA PCR Primer, Index 1 (100% over 27bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC3790131.5465132382297038RNA PCR Primer, Index 1 (100% over 24bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC3756501.5327909542442824RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG3323151.3559681244767436No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA3297491.3454978953044001No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGC2504211.021810311600712RNA PCR Primer, Index 15 (100% over 50bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT2335270.95287654245123RNA PCR Primer, Index 1 (100% over 25bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT1694820.6915492519825089No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA1602640.6539364022121806RNA PCR Primer, Index 1 (100% over 22bp)
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA1340920.5471449610981614RNA PCR Primer, Index 1 (100% over 28bp)
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA1316780.5372949481511476RNA PCR Primer, Index 1 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG1309780.5344386892187079No Hit
GGCCGAGGTGGGATCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1157540.4723191378080465RNA PCR Primer, Index 1 (100% over 32bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT1122010.457821583540963RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA940390.3837139053538615RNA PCR Primer, Index 1 (100% over 23bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCA936060.38194710518565234RNA PCR Primer, Index 15 (100% over 39bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA906850.37002834469757157RNA PCR Primer, Index 1 (100% over 23bp)
TGCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCA812900.3316932694543264No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAA791420.3229286349016398RNA PCR Primer, Index 15 (100% over 40bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA767200.3130459789953982No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA636950.2598991610025012RNA PCR Primer, Index 1 (100% over 22bp)
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA628770.25656141842145014No Hit
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA536270.2188179968142104RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC524150.2138725884911861RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGAACT503430.20541806205116442RNA PCR Primer, Index 1 (100% over 25bp)
TTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTC470790.19209973468618813RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGA437620.17856514771632714RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAAC397460.16217838218392985RNA PCR Primer, Index 1 (100% over 24bp)
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT390390.15929356066216568RNA PCR Primer, Index 1 (100% over 25bp)
GGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTC390010.15913850660583326RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG381570.1556946744072916No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG360420.14706469206142No Hit
CCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGAAC352770.14394320908525368RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG315960.12892336747052402Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA312500.12751155948391807RNA PCR Primer, Index 1 (100% over 28bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC272240.11108399025248596No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT248990.10159713022688244RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)