FastQCFastQC Report
Tue 18 Jul 2023
SRR10530136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31105770
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC386930512.439187327624426RNA PCR Primer, Index 16 (100% over 50bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA323008410.384195601009074RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG16216215.213248217292161Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC12346003.969038541723931RNA PCR Primer, Index 1 (100% over 26bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT7734332.486461515017953RNA PCR Primer, Index 1 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA6653852.1391047384456323No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG6039291.9415336768708829No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG5094751.6378794030818076Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2980410.9581534229822954No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA2977770.9573047058471787No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2606030.8377963316773704No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC2104400.6765304314922923RNA PCR Primer, Index 1 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2020700.6496222405039322RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA1509350.4852315181395606RNA PCR Primer, Index 1 (100% over 22bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1462330.4701153515891103RNA PCR Primer, Index 1 (100% over 25bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC1121990.36070156758697824RNA PCR Primer, Index 1 (100% over 24bp)
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA1077880.34652091878773617RNA PCR Primer, Index 1 (100% over 28bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA1077260.34632159885448904No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG1046260.33635560219213345Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA842660.2709015079838885RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA765460.24608296145699013RNA PCR Primer, Index 1 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG737110.2369688967673843No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT643790.20696803197606103RNA PCR Primer, Index 16 (100% over 37bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG627640.2017760691987371No Hit
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC593990.19095814056363175RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA580010.1864637975526727RNA PCR Primer, Index 1 (100% over 22bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC577810.18575653327340874RNA PCR Primer, Index 1 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT571580.1837536894280386RNA PCR Primer, Index 1 (100% over 25bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG564150.18136506506670627No Hit
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA466780.15006219103401072RNA PCR Primer, Index 1 (100% over 22bp)
TGAGTCTGAAACCAATTTTTTGAGGCCTTGGAATTCTCGGGTGCCAAGGA463190.1489080643237573RNA PCR Primer, Index 1 (100% over 22bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT456470.14674769343436925RNA PCR Primer, Index 1 (100% over 25bp)
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA441630.14197687438697065RNA PCR Primer, Index 1 (100% over 23bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCA400780.12884426265609242RNA PCR Primer, Index 16 (100% over 40bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA386000.12409273263449194RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA371760.11951480384507439No Hit
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC367530.11815492752630782RNA PCR Primer, Index 2 (100% over 34bp)
CCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC356770.11469576223318054RNA PCR Primer, Index 1 (100% over 27bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC343370.1103878798049365RNA PCR Primer, Index 1 (100% over 24bp)
GGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTC339100.10901514413563787RNA PCR Primer, Index 1 (100% over 26bp)
CGGCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC334900.10766491232977032RNA PCR Primer, Index 1 (100% over 27bp)
GCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC318920.10252760179220768RNA PCR Primer, Index 1 (100% over 27bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC316650.10179783364951261RNA PCR Primer, Index 16 (100% over 39bp)
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT312750.10054404697263561RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)