FastQCFastQC Report
Tue 18 Jul 2023
SRR10530137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32426144
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC392906712.11697265021706RNA PCR Primer, Index 16 (100% over 50bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA345920610.667953611752294RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG17331405.344884670838444Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC13213224.074866256067943RNA PCR Primer, Index 1 (100% over 26bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT8270062.550429677978362RNA PCR Primer, Index 1 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA7117912.195114534740856No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG6489572.001338796250334No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG5453951.681960704300826Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG3183160.9816646715687194No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA3169670.9775044482624885No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2750310.8481767058087449No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC2249280.6936624965336613RNA PCR Primer, Index 1 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2001320.6171933363399608RNA PCR Primer, Index 1 (100% over 24bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1536120.47372885286637845RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA1421360.4383376574161886RNA PCR Primer, Index 1 (100% over 22bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC1187450.36620142068079387RNA PCR Primer, Index 1 (100% over 24bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA1141900.35215411366827953No Hit
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA1130800.34873094993965364RNA PCR Primer, Index 1 (100% over 28bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG1038250.3201891658780026Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA920210.2837864409656603RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA824410.2542423792357179RNA PCR Primer, Index 1 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG730510.22528426445031516No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG636200.1961997084821433No Hit
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC635760.19606401550551306RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT621110.19154605616998432RNA PCR Primer, Index 16 (100% over 37bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA612670.18894321816371382RNA PCR Primer, Index 1 (100% over 22bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC609730.18803654236532102RNA PCR Primer, Index 1 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT603150.18600731557844188RNA PCR Primer, Index 1 (100% over 25bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG569580.17565455824781387No Hit
TGAGTCTGAAACCAATTTTTTGAGGCCTTGGAATTCTCGGGTGCCAAGGA494910.1526268433274089RNA PCR Primer, Index 1 (100% over 22bp)
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA484710.14948123341461755RNA PCR Primer, Index 1 (100% over 22bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT479620.14791151238950892RNA PCR Primer, Index 1 (100% over 25bp)
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA477750.1473348172388305RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA435830.13440697728351542No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCA411710.12696853501914998RNA PCR Primer, Index 16 (100% over 40bp)
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC387530.11951158916706224RNA PCR Primer, Index 2 (100% over 34bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA385550.11890097077222628RNA PCR Primer, Index 1 (100% over 22bp)
CCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC370210.11417022017789102RNA PCR Primer, Index 1 (100% over 27bp)
GGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTC360990.1113268355312306RNA PCR Primer, Index 1 (100% over 26bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC360330.11112329606628528RNA PCR Primer, Index 1 (100% over 24bp)
CGGCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC353500.10901697099722989RNA PCR Primer, Index 1 (100% over 27bp)
GCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC345110.10642955264739465RNA PCR Primer, Index 1 (100% over 27bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC330160.10181907537325438RNA PCR Primer, Index 16 (100% over 39bp)
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT327470.10098949785703783RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)