FastQCFastQC Report
Tue 18 Jul 2023
SRR10530138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33772754
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG379556211.238532694135634No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT366477810.851285625092938No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA17907275.302283017843319No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA9096232.693363413596652No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC8427772.495434633491838No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG6833312.0233203368608907No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA4995541.4791627594243573No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG3463941.025661099476815No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA3247970.9617131016321618No Hit
AACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3239550.9592199676697968RNA PCR Primer, Index 1 (100% over 31bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG2403680.7117216440210947Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1656420.4904604463112484Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA1606840.4757799734069658No Hit
TTTGGTGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA1556030.46073530159844234RNA PCR Primer, Index 1 (100% over 40bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1466410.43419911802277067No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC1354100.40094450100219836No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG1282790.3798298474563253No Hit
TTTGGTGACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC1223570.3622950026521379RNA PCR Primer, Index 1 (100% over 38bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA1024040.303214834064169No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT953990.2824732623226403No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT887800.26287462372775405No Hit
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT826500.24472389784972823RNA PCR Primer, Index 1 (100% over 43bp)
GCCGCCTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG824440.24411393870929213RNA PCR Primer, Index 1 (100% over 39bp)
TTTGGTGACTCTAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA765660.2267093764399551RNA PCR Primer, Index 1 (100% over 34bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAG759760.22496240608627893No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT734180.21738825326474706No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG644170.19073659198773069No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC640270.18958181497428372No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC637560.1887793929982731No Hit
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA632080.187156783246045RNA PCR Primer, Index 1 (100% over 37bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC593760.17581035884725302RNA PCR Primer, Index 1 (100% over 24bp)
CGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT580350.17183970250101605RNA PCR Primer, Index 1 (100% over 41bp)
AACCTCGGGCCGATCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC577970.17113499242614327RNA PCR Primer, Index 1 (100% over 33bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA572270.16944724140648998RNA PCR Primer, Index 1 (100% over 40bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTG565560.16746043275002093No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAA564510.16714953124640056No Hit
TTTGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT542580.1606561312707871RNA PCR Primer, Index 1 (100% over 41bp)
AGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCC537090.15903056055185785RNA PCR Primer, Index 1 (100% over 27bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG534250.15818964600873237No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAAC518600.15355573312143866No Hit
GGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC498860.1477107848533762RNA PCR Primer, Index 1 (100% over 38bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC463640.1372822601319395No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA456790.13525399794165438RNA PCR Primer, Index 1 (100% over 22bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA438950.12997163334680967RNA PCR Primer, Index 1 (100% over 22bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC422840.12520151599126325No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGTGGAATTCTCGGGTGCCAA411210.12175791171783029No Hit
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC405670.12011753616539533RNA PCR Primer, Index 1 (100% over 38bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC395310.11704997466300794No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG381740.11303194284955262No Hit
TTCCCGTGGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAAT365140.10811673812565004No Hit
TTGGTGACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG363980.1077732659883171RNA PCR Primer, Index 1 (100% over 39bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC360870.10685240534426065RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCAC356870.10566801866380218No Hit
AACCTCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA351330.10402764311136722RNA PCR Primer, Index 1 (100% over 40bp)
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA350420.10375819514156293RNA PCR Primer, Index 1 (100% over 34bp)
CGTTCCCGTGGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGA346700.10265671552873656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)